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Contracting - Request For Quotes

Project: DNA barcoding analysis of goose fecal samples

Requirements:

Scope: The contractor will conduct plant DNA barcoding on a total of 760 fecal samples from geese.  These results will be used to determine geese diets for plants and invertebrates as well as the presence of intestinal parasites.

Technical Requirements:

Forage Plant Analysis
The contractor will conduct an analysis using ITS2 primers to reconstruct plants in the diet from 260 goose fecal samples, and conduct 40 amplification replicates and 40 subsample replicates to assess repeatability of the approach.
The contractor will conduct an analysis using trnL primers to reconstruct plants in the diet from 34 goose fecal samples.
Invertebrate Foods Analysis
The contractor will conduct an analysis to reconstruct invertebrate foods in the diet from 153 goose fecal samples, and conduct 20 amplification replicates and 20 subsample replicates to assess repeatability of the approach.
Intestinal Parasite Analysis
The contractor will conduct an analysis to identify the presence of intestinal parasites from 153 goose fecal samples, and conduct 20 amplification replicates and 20 subsample replicates to assess repeatability of the approach.
Criteria for acceptance of Product or Service: The contractor’s compliance will be measured based on timely completion of analyses and a combination of analytical results and assessments of sequencing quality and performance statistics.

Deliverables:

Contractor will deliver sequencing results in an excel spreadsheet along with any assessments of sequencing quality and performance statistics. Final product should consist of and contractor will deliver a list of plant species that are found
in each sample in an excel spreadsheet. Specific deliverables include:

Fecal plant diet reconstruction, ITS2 primer (qty = 340)
Fecal plant diet reconstruction, ITS2 primer, amplification replicate (qty = 40)
Fecal plant diet reconstruction, ITS2 primer, sub-sample replicate (qty = 40)
Fecal plant diet reconstruction, trnL primer, (qty = 34)
Fecal invertebrate diet reconstruction (qty = 153)
Fecal invertebrate diet reconstruction, amplification replicate (qty = 20)
Fecal invertebrate diet reconstruction, sub-sample replicate (qty = 20)
Fecal intestinal parasite detection (qty = 153)
Fecal intestinal parasite detection, amplification replicate (qty = 20)
Fecal intestinal parasite detection, sub-sample replicate (qty = 20)

Delivery dates for work/product to be completed and point of delivery: Government shall deliver fecal samples via overnight shipping immediately after contract award. Government shall also specify which samples will be used for amplification and sub-sample replicates. Vendor will submit results of all analyses to the government no later than 30 September, 2017.

SUPPORTING INFORMATION:

A.        Place of Performance: Performance will be at the contractor’s facility.

B.        Period of Performance: Award of contract 30 September, 2017.

C.        Government Furnished Property (GFP):  N/A

D.        Special Considerations. N/A

Period of Performance:  January 25, 2017 to September 30, 2017 
Vendor must be registered in System for Award Management (SAM) sam.gov
Vendor must invoice through the Invoice Processing Platform (IPP) ipp.gov


Interested parties should contact USGS Purchasing Agent Tyrone Rowse trowse@usgs.gov.

Quote Due By: January 20th, 2017 NLT 12:00PM AKST

Project: Provide animal husbandry and veterinary oversight necessary to conduct an experiment to determine whether a virus is the causative agent of avian keratin disorder in Black-capped Chickadees

Requirements:

General Objectives:  Objectives for the current phase of this research project are to:

Determine whether Poecivirus replicates in an avian host, determine if is transmissible between avian hosts, and identify the mode of transmission.
Determine if experimental infection with Poecivirus causes clinical signs of Avian keratin disorder (AKD), an epizootic of beak deformities that appears to be affecting a broad range of avian species, from passerines to raptors, across continents. AKD.

Technical Requirements:

The contractor must meet all state and federal animal care guidelines and adhere to standard operating procedures and protocols for small passerine husbandry. Veterinary staff will follow experimental design provided by government. During the laboratory experiment, buccal and cloacal swabs must be collected weekly until uninfected individuals become infected; then they will be taken every other week to monitor the course of infection. In addition, beak measurements must be taken every other week to monitor the development of beak overgrowth.

Deliverables: 

Documentation of veterinary inspections and a log of weekly activities, including beak measurements and sample collection, will be collected throughout the study and provided to PI at least once every two months.
Cloacal and buccal swab samples will be  shipped monthly for analysis by PI or collaborators.
A final summary of results will be provided by email or through a secured website by March 31, 2018. This report should include all veterinary reports and complete summary of all measurements, swab collections, and other sample data.

Special Considerations:

    All animal care will be conducted with appropriate personal protective equipment by trained veterinary staff. Prior to start of experiment, all procedures must be approved by the relevant Animal Care and Use Committees.
  1. As requested, biological samples will be sent to collaborating laboratories for additional analyses.
  2. All data will be the property of the government.
  3. All work will be performed at the vendor’s place of business.  Any necessary consultations can occur via telephone or email.

Period of Performance:  March 01, 2017 to February 28, 2018
Vendor must be registered in System for Award Management (SAM) sam.gov
Vendor must invoice through the Invoice Processing Platform (IPP) ipp.gov

Interested parties should contact USGS Purchasing Agent Tyrone Rowse trowse@usgs.gov.

Quote Due By: January 24, 2017 NLT 12:00PM AKST

For all projects, contact: Tyrone Rowse

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Page Last Modified: December 05 2016 16:04:05.