USGS - science for a changing world

Alaska Science Center

white dothome: white dotproducts: white dotoutreach/media: white dotcontact us:   white dotinternal:
All USGS This site only

Development and characterization of 21 polymorphic microsatellite markers for the barren-ground shrew, Sorex ugyunak (Mammalia: Sorcidae), through next-generation sequencing, and cross-species amplification in the masked shrew, Sorex cinereus

Start a New Search | Return to existing search by pressing your browser's back arrow

Full Publication: http://dx.doi.org/10.1007/s12686-012-9792-5

Product Type: Journal Article

Year: 2013

Authors: Sonsthagen, S. A., G. K. Sage, M. C. Fowler, A. G. Hope, J. A. Cook, and S. L. Talbot


Suggested Citation:
Sonsthagen, S. A., G. K. Sage, M. C. Fowler, A. G. Hope, J. A. Cook, and S. L. Talbot. 2013. Development and characterization of 21 polymorphic microsatellite markers for the barren-ground shrew, Sorex ugyunak (Mammalia: Sorcidae), through next-generation sequencing, and cross-species amplification in the masked shrew, Sorex cinereus. Conservation Genetics Resources 5(2):315-318. doi:10.1007/s12686-012-9792-5

Abstract


We used next generation shotgun sequencing to develop 21 novel microsatellite markers for the barren-ground shrew (Sorex ugyunak), which were polymorphic among individuals from northern Alaska. The loci displayed moderate allelic diversity (averaging 6.81 alleles per locus) and heterozygosity (averaging 70 %). Two loci deviated from Hardy-Weinberg equilibrium (HWE) due to heterozygote deficiency. While the population did not deviate from HWE overall, it showed significant linkage disequilibrium suggesting this population is not in mutation-drift equilibrium. Nineteen of 21 loci were polymorphic in masked shrews (S. cinereus) from interior Alaska and exhibited linkage equilibrium and HWE overall. All loci yielded sufficient variability for use in population studies.

Accessibility FOIA Privacy Policies and Notices

USA.gov logo U.S. Department of the Interior | U.S. Geological Survey
URL: https://alaska.usgs.gov/products/pubs/info.php?pubid=1810
Page Contact Information: ascweb@usgs.gov
Page Last Modified: March 20 2017 13:08:43.