ASC Data Repository
This data set is comprised of one table with sampling information and NCBI BioProject accession numbers for sequence information of this amplicon-based study targeting Labyrinthula, Phytophthora, and Halophytophthora of known pathogenic and non-pathogenic [to eelgrass (Zostera marina)] strains from eDNA samples. eDNA samples included water, sediment, and eelgrass from Notsuke Wan (Cove) Japan, Safety Sound, Izembek Lagoon, Port Moller, Chignik Lagoon, and Frederick Sound, Alaska and cloacal swabs from waterfowl hunted near Cold Bay Alaska. Replicate samples and multiple sampling dates of the same location were included. Highly conserved primers which could differentiate species of interest were developed for four portions of mtDNA genes (5.8S, 18S, ITS, and COI). The reference list and conserved primer sets to identify species present were developed using publicly available data https://www.ncbi.nlm.nih.gov/genbank/
Version History:First release: April 2020 Revised: April 2021 (ver. 2.0)
Menning, D. M., D. H. Ward, S. Wyllie-Echeverria, G. K. Sage, M. C. Gravley, H. A. Gravley, and S. L. Talbot. 2020. Are migratory waterfowl vectors of seagrass pathogens? . Ecology and Evolution 10(4):2062-2073. doi:10.1002/ece3.6039
Menning, D. M., H. A. Gravley, M. N. Cady, D. Pepin, S. Wyllie-Echeverria, D. H. Ward, and S. L. Talbot. 2021. Metabarcoding of environmental samples suggest wide distribution of eelgrass (Zostera marina) pathogens in the north Pacific. Metabarcoding and Metagenomics 5:e62823. doi:10.3897/mbmg.5.62823
DataID: 293 | doi:10.5066/P9T69I3V | Date Posted Online: 2020-04-27 | Last Updated: 2021-04-27 15:56:08
