Spectacled Eider (Somateria fischeri) Microsatellite and Mitochondrial DNA Data, 2014-2018, Alaska and Russia.

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Metadata:

Identification_Information:
Citation:
Citation_Information:
Originator: Sonsthagen, S.A. (ORCID: 0000-0001-6215-5874)
Originator: Haughey, C.L. (ORCID: 0000-0002-4846-6008)
Originator: Sexson, M.G. (ORCID: 0000-0002-1078-0835)
Originator: Solovyeva, D.
Originator: Petersen, M.R. (ORCID: 0000-0001-6082-3189)
Originator: Powell, A.N. (ORCID: 0000-0002-9783-134X)
Publication_Date: 20191112
Title:
Spectacled Eider (Somateria fischeri) Microsatellite and Mitochondrial DNA Data, 2014-2018, Alaska and Russia.
Geospatial_Data_Presentation_Form: tabular digital data
Publication_Information:
Publication_Place: Anchorage, Alaska
Publisher: U.S. Geological Survey, Alaska Science Center
Other_Citation_Details:
Suggested citation: Sonsthagen, S.A., Haughey, C.L., Sexson, M.G., Solovyeva, D. Petersen, M.R., Powell, A.N., 2019, Spectacled eider (Somateria fischeri) microsatellite and mitochondrial DNA data, 2014-2018, Alaska and Russia: U.S. Geological Survey data release, https://doi.org/10.5066/P9F8DV8O
Online_Linkage: https://doi.org/10.5066/P9F8DV8O
Larger_Work_Citation:
Citation_Information:
Originator: U.S. Geological Survey, Alaska Science Center
Publication_Date: 2000
Title:
Developing and Applying Molecular Tools to Natural Resource Problems in Alaska
Geospatial_Data_Presentation_Form: website
Series_Information:
Series_Name: Alaska Science Portal
Issue_Identification: 378
Publication_Information:
Publication_Place: Anchorage, Alaska
Publisher: U.S. Geological Survey, Alaska Science Center
Other_Citation_Details:
This is a link to the broader USGS Alaska Science Center research project supported by these data. Users will find a description of the research project and links to associated reports, publications, and data products.
Online_Linkage: https://alaska.usgs.gov/portal/project.php?project_id=378
Description:
Abstract:
This data set describes nuclear microsatellite genotypes derived from ten autosomal loci (Aph8, Aph16, Cmo7, Cmo9, Hhi5, Sfi10, Smo4, Smo6, Smo8, Smo12) and nucleotide sequence data derived from one mitochondrial DNA locus (control region). A total of 262 Spectacled Eiders were examined for this study. Samples were collected at Indigirka and Chaun River Deltas, Russia, and Yukon-Kuskokwim Delta, Utqiagvik, Colville River Delta, Prudhoe Bay, Alaska. Samples used in the study originated from blood or feather samples collected in the field from live trapped birds or from tissue taken from dead birds.
Purpose:
To examine spatial genetic structure and temporal changes in genetic diversity within Spectacled Eiders.
Time_Period_of_Content:
Time_Period_Information:
Range_of_Dates/Times:
Beginning_Date: 2010
Ending_Date: 2018
Currentness_Reference: observed
Status:
Progress: Complete
Maintenance_and_Update_Frequency: None planned
Spatial_Domain:
Description_of_Geographic_Extent:
Yukon-Kuskokwim Delta north to Colville River Delta, Alaska, USA, west to Indigirka Delta, Russia.
Bounding_Coordinates:
West_Bounding_Coordinate: 152.58
East_Bounding_Coordinate: -150.89
North_Bounding_Coordinate: 71.36
South_Bounding_Coordinate: 60.84
Keywords:
Theme:
Theme_Keyword_Thesaurus: USGS Metadata Identifier
Theme_Keyword: USGS:ASC236
Theme:
Theme_Keyword_Thesaurus: ISO 19115 Topic Category
Theme_Keyword: biota
Theme:
Theme_Keyword_Thesaurus: NASA GCMD Earth Science Thesaurus
Theme_Keyword: Earth Science
Theme_Keyword: Biological Classification
Theme_Keyword: Animals/Vertebrates
Theme_Keyword: Birds
Theme_Keyword: Waterfowl
Theme:
Theme_Keyword_Thesaurus: USGS CSA Biocomplexity Thesaurus
Theme_Keyword: Birds
Theme_Keyword: Population genetic
Theme_Keyword: Genetic variance
Theme_Keyword: Genetic diversity
Theme:
Theme_Keyword_Thesaurus: USGS Thesaurus
Theme_Keyword: Ecological Processes
Theme_Keyword: Life Sciences
Theme_Keyword: Genetics
Theme_Keyword: DNA sequencing
Theme_Keyword: Polymerase chain reaction
Theme_Keyword: Systematics and taxonomy
Theme_Keyword: Evolution
Theme:
Theme_Keyword_Thesaurus: MeSH-National Library of Medicine
Theme_Keyword: Arctic Regions
Theme_Keyword: North America
Theme_Keyword: Alaska
Theme_Keyword: Canada
Theme_Keyword: DNA
Theme_Keyword: Alleles
Theme_Keyword: Genetic markers
Theme_Keyword: Microsatellite Repeats
Theme_Keyword: DNA, Mitochondrial
Theme_Keyword: Genetics, Population
Theme:
Theme_Keyword_Thesaurus: None
Theme_Keyword: Aph8
Theme_Keyword: Aph16
Theme_Keyword: Cmo7
Theme_Keyword: Cmo9
Theme_Keyword: Hhi5
Theme_Keyword: Sfi10
Theme_Keyword: Smo4
Theme_Keyword: Smo6
Theme_Keyword: Smo8
Theme_Keyword: Smo12
Place:
Place_Keyword_Thesaurus: USGS Geographic Names Information System
Place_Keyword: Alaska
Place_Keyword: Yukon-Kuskokwim Delta
Place_Keyword: Utqiagvik
Place_Keyword: Colville River Delta
Place_Keyword: Prudhoe Bay
Place:
Place_Keyword_Thesaurus: NGIA GeoNames
Place_Keyword: Russia
Place_Keyword: Indigirka
Place_Keyword: Chaun
Place:
Place_Keyword_Thesaurus: none
Place_Keyword: Indigirka River Delta
Place_Keyword: Chaun River Delta
Taxonomy:
Keywords/Taxon:
Taxonomic_Keyword_Thesaurus: None
Taxonomic_Keywords: Animals
Taxonomic_Keywords: Birds
Taxonomic_Keywords: Ducks
Taxonomic_System:
Classification_System/Authority:
Classification_System_Citation:
Citation_Information:
Originator: ITIS Integrated Taxonomic Information System
Publication_Date: Unknown
Title: ITIS Integrated Taxonomic Information System
Geospatial_Data_Presentation_Form: database
Series_Information:
Series_Name: Taxonomic Serial Number
Issue_Identification: TSN 175161
Publication_Information:
Publication_Place: online
Publisher: ITIS-North America
Other_Citation_Details:
Taxonomic details for Spectacled eider were retrieved September 23, 2019, from the Integrated Taxonomic Information System on-line database http://www.itis.gov
Online_Linkage: http://www.itis.gov/
Taxonomic_Procedures:
Species were identified by skilled observers in the field based on general appearance of feathers and plumage, behavior, and vocalization.
Taxonomic_Classification:
Taxon_Rank_Name: Kingdom
Taxon_Rank_Value: Animalia
Taxonomic_Classification:
Taxon_Rank_Name: Phylum
Taxon_Rank_Value: Chordata
Taxonomic_Classification:
Taxon_Rank_Name: Subphylum
Taxon_Rank_Value: Vertabrata
Taxonomic_Classification:
Taxon_Rank_Name: Superclass
Taxon_Rank_Value: Tetrapoda
Taxonomic_Classification:
Taxon_Rank_Name: Class
Taxon_Rank_Value: Aves
Taxonomic_Classification:
Taxon_Rank_Name: Order
Taxon_Rank_Value: Anseriformes
Taxonomic_Classification:
Taxon_Rank_Name: Family
Taxon_Rank_Value: Anatidae
Taxonomic_Classification:
Taxon_Rank_Name: Genus
Taxon_Rank_Value: Somateria
Taxonomic_Classification:
Taxon_Rank_Name: Species
Taxon_Rank_Value: fischeri
Applicable_Common_Name: Spectacled eider
Applicable_Common_Name: TSN 175161
Access_Constraints: None
Use_Constraints:
It is requested that the authors and the USGS Alaska Science Center be cited for any subsequent publications that reference this dataset.
Point_of_Contact:
Contact_Information:
Contact_Organization_Primary:
Contact_Organization: U.S. Geological Survey, Alaska Science Center
Contact_Address:
Address_Type: mailing and physical
Address: 4210 University Drive
City: Anchorage
State_or_Province: Alaska
Postal_Code: 99508
Country: USA
Contact_Voice_Telephone: 907-786-7000
Contact_Electronic_Mail_Address: ascweb@usgs.gov
Cross_Reference:
Citation_Information:
Originator: Sonsthagen, S.A. (ORCID: 0000-0001-6215-5874)
Originator: Haughey, C.L. (ORCID: 0000-0002-4846-6008)
Originator: Sexson, M.G. (ORCID: 0000-0002-1078-0835)
Originator: Solovyeva, D.
Originator: Petersen, M.R. (ORCID: 0000-0001-6082-3189)
Originator: Powell, A.N. (ORCID: 0000-0002-9783-134X)
Publication_Date: 2020
Title:
Temporal variation in genetic structure within the threatened spectacled eider
Geospatial_Data_Presentation_Form: journal article
Series_Information:
Series_Name: Conservation Genetics
Issue_Identification: 21
Publication_Information:
Publication_Place: online
Publisher: Springer Nature
Other_Citation_Details:
Sonsthagen, S.A., Haughey, C.L., Sexson, M.G., Solovyeva, D. Petersen, M.R., Powell, A.N., 2020. Temporal variation in genetic structure within the threatened spectacled eider. Conservation Genetics 21: doi:10.1007/s10592-019-01234-9
Online_Linkage: https://doi.org/10.1007/s10592-019-01234-9
Data_Quality_Information:
Attribute_Accuracy:
Attribute_Accuracy_Report:
Identifying information about samples were cross-checked with original field data. Polymerase chain reaction (PCR) amplification and electrophoresis followed protocols described in Sonsthagen et al 2004. Quality control (QC) included a minimum of 10% re-extraction and PCR replication for each locus and all laboratory techniques were performed using sterile procedures. All duplicate characterizations of the same individual resulted in concordant data. GenBank accession numbers were cross-referenced to the appropriate individual samples as recorded.
Logical_Consistency_Report:
For microsatellite and mitochondrial DNA loci, reported allele sizes were reviewed to confirm they conform to expected distributions (e.g. dinucleotide repeats with 1 base pair differences were reviewed on gel images with laboratory personnel, gaps in allele sizes were reviewed, and single occurrences of an allele size were scrutinized). No inconsistencies in allele scores were observed between replicates. Tests for null alleles and allelic dropout were implemented in MicroChecker (van Oosterhout et al 2004).
Completeness_Report: Blank cells are described for each attribute.
Lineage:
Methodology:
Methodology_Type: Field
Methodology_Description:
Blood samples were collected from the jugular or brachial vein and stored in preservation buffer. Feathers were collected from nests and stored in paper envelopes.
Methodology:
Methodology_Type: Lab
Methodology_Description:
DNA were extracted by a modified salt extraction technique (Medrano et al 1990; Sonsthagen et al 2004) or using a Qiagen Dneasy Blood and Tissue extraction kit (QIAGEN, Düsseldorf, Germany) following manufacturer’s protocol.
Methodology:
Methodology_Type: Lab
Methodology_Description:
Polymerase chain reaction (PCR) amplification and scoring of microsatellite loci followed Sonsthagen et al 2004 except the thermocycler parameters consisted of a 2 min denaturation at 94 °C, followed 40 cycles at 94°C for 15 seconds, 50°C for 15 seconds and 72 °C for 30 seconds with a final extension at 72 °C for 30 min. Microsatellite alleles were resolved on a 25 cm 6% polyacrylamide gel using a LI-COR (IR 4200) automated DNA sequencer. For quality control purposes, we extracted, amplified, and sequenced, 10% of the samples in duplicate.
Source_Information:
Source_Citation:
Citation_Information:
Originator: Longmire, J.L.
Originator: Lewis, A.K.
Originator: Brown, N.C.
Originator: Buckingham, J.M.
Originator: Clark, L.M.
Originator: Jones, M.D.
Originator: Meincke, L.J.
Originator: Meyne, J.
Originator: Ratliff, R.L.
Originator: Ray, F.A.
Originator: Wagner, R.P.
Originator: Moyzis, R.K.
Publication_Date: 1988
Title:
Isolation and molecular characterization of a highly polymorphic centromeric tandem repeat in the family Falconidae
Geospatial_Data_Presentation_Form: journal article
Series_Information:
Series_Name: Genomics
Issue_Identification: 2(1):14-24
Publication_Information:
Publication_Place: online
Publisher: Elsevier
Other_Citation_Details:
Longmire, J.L., Lewis, A.K., Brown, N.C., Buckingham, J.M., Clark, L.M., Jones, M.D., Meincke, L.J., Meyne, J., Ratliff, R.L., Ray, F.A., Wagner, R.P., Moyzis, R.K. 1988. Isolation and molecular characterization of a highly polymorphic centromeric tandem repeat in the family Falconidae. Genomics 2(1):14-24 doi:10.1016/0888-7543(88)90104-8
Online_Linkage: https://doi.org/10.1016/0888-7543(88)90104-8
Type_of_Source_Media: publication
Source_Time_Period_of_Content:
Time_Period_Information:
Single_Date/Time:
Calendar_Date: 1988
Source_Currentness_Reference: publication date
Source_Citation_Abbreviation: Longmire et al 1988
Source_Contribution:
Laboratory Technique Citation: describes blood preservation buffer.
Source_Information:
Source_Citation:
Citation_Information:
Originator: Medrano, J.F.
Originator: Aasen, E.
Originator: Sharrow, L.
Publication_Date: 1990
Title: DNA extraction from nucleated red blood cells
Geospatial_Data_Presentation_Form: journal article
Series_Information:
Series_Name: Biotechniques
Issue_Identification: 8(1):43
Publication_Information:
Publication_Place: online
Publisher: Future Science, Ltd.
Other_Citation_Details:
Medrano, J.F., Aasen, E., Sharrow, L. 1990. DNA extraction from nucleated red blood cells. Biotechniques 8(1):43
Type_of_Source_Media: publication
Source_Time_Period_of_Content:
Time_Period_Information:
Single_Date/Time:
Calendar_Date: 1990
Source_Currentness_Reference: publication date
Source_Citation_Abbreviation: Medrano et al 1990
Source_Contribution:
Laboratory Technique Citation: describes DNA extraction technique.
Source_Information:
Source_Citation:
Citation_Information:
Originator: Sonsthagen, S.A.
Originator: Talbot, S.L.
Originator: White, C.M.
Publication_Date: 2004
Title:
Gene flow and genetic characterization of Northern Goshawks breeding in Utah.
Geospatial_Data_Presentation_Form: journal article
Series_Information:
Series_Name: Condor
Issue_Identification: 106(4):826–836
Publication_Information:
Publication_Place: Oxford, UK
Publisher: Oxford University Press
Other_Citation_Details:
Sonsthagen, S.A., Talbot, S.L., White, C.M. 2004. Gene flow and genetic characterization of Northern Goshawks breeding in Utah. Condor 106(4):826–836.
Online_Linkage: http://www.jstor.org/stable/3247787
Type_of_Source_Media: publication
Source_Time_Period_of_Content:
Time_Period_Information:
Single_Date/Time:
Calendar_Date: 2004
Source_Currentness_Reference: publication date
Source_Citation_Abbreviation: Sonsthagen et al 2004
Source_Contribution:
Laboratory Technique Citation: describes polymerase chain reaction (PCR) amplification and electrophoresis protocols.
Source_Information:
Source_Citation:
Citation_Information:
Originator: van Oosterhout, C.
Originator: Hutchinson, W.F.
Originator: Wills, D.P.M.
Originator: Shipley, P.
Publication_Date: 2004
Title:
MICRO-CHECKER: software for identifying and correcting genotyping errors in microsatellite data
Geospatial_Data_Presentation_Form: journal article
Series_Information:
Series_Name: Molecular Ecology Notes
Issue_Identification: 4(3):535-538
Publication_Information:
Publication_Place: online
Publisher: Wiley Online Library
Other_Citation_Details:
van Oosterhout, C., Hutchinson, W.F., Wills, D.P.M., Shipley, P. 2004. MICRO-CHECKER: software for identifying and correcting genotyping errors in microsatellite data. Molecular Ecology Notes 4(3):535-538 doi:10.1111/j.1471-8286.2004.00684.x
Online_Linkage: https://doi.org/10.1111/j.1471-8286.2004.00684.x
Type_of_Source_Media: publication
Source_Time_Period_of_Content:
Time_Period_Information:
Single_Date/Time:
Calendar_Date: 2004
Source_Currentness_Reference: publication date
Source_Citation_Abbreviation: van Oosterhout et al 2004
Source_Contribution:
Laboratory Technique Citation: describes tool used to identify potential scoring errors and null alleles of microsatellite loci.
Source_Information:
Source_Citation:
Citation_Information:
Originator: Unknown
Publication_Date: Unknown
Title: 4 M Urea Tissue Preservation Buffer Recipe
Geospatial_Data_Presentation_Form: document
Series_Information:
Series_Name: Unknown
Issue_Identification: Unknown
Publication_Information:
Publication_Place: Unknown
Publisher: Unknown
Other_Citation_Details:
Current and past ASC personnel as well as many associates familiar with the buffer are unsure of the origin; use at the Alaska Science Center for tissue preservation is known from at least 1996.
Type_of_Source_Media: paper
Source_Time_Period_of_Content:
Time_Period_Information:
Single_Date/Time:
Calendar_Date: 1996
Source_Currentness_Reference: observed
Source_Citation_Abbreviation: MEL Tissue Preservation Buffer
Source_Contribution:
Laboratory Technique Citation: Tissue Preservation Buffer. This buffer is often used to preserve non-blood tissue samples collected for DNA analysis at ambient temperatures until DNA extraction can occur. Current and past personnel of the Alaska Science Center as well as many associates familiar with the buffer are unsure of its origins, though its use at the Alaska Science Center for tissue preservation is known from at least 1996. Its formulation is (4.00 M Urea, 0.2 M NaCl, 10 mM EDTA, 0.5% N-Lauroyl-Sarcosine, 100 mM Tris-HCl, pH 8.0) stored at room temperature.
Process_Step:
Process_Description:
Initial microsatellite allele sizes were determined by scoring based on PCR product size relative to an M13 DNA sequence ladder using Gene Profiler 4.05 (Scanalytics, Inc., Fairfax, VA). Subsequent gels included these known size individuals from which relative sizes of unknown individuals were assigned using Gene Profiler 4.05. For this reason, these microsatellite data cannot be combined with data generated outside the USGS Alaska Science Center without molecular size calibration between laboratories. MtDNA sequences from opposite strands were reconciled using Sequencher 4.1.2 (Gene Codes Corporation, Ann Arbor, MI). Tests for null alleles and allelic dropout were implemented in MicroChecker (van Oosterhout et al 2004).
Process_Date: Unknown
Process_Step:
Process_Description:
Sequences of novel microsatellite loci developed in this study were submitted to the NCBI Genbank publicly accessible genetic sequence data database.
Source_Produced_Citation_Abbreviation: NCBI GenBank
Process_Date: Unknown
Spatial_Data_Organization_Information:
Indirect_Spatial_Reference:
River deltas and cities names are listed to describe generalized field sampling locations.
Entity_and_Attribute_Information:
Detailed_Description:
Entity_Type:
Entity_Type_Label: spectacledEider_microsatellite_Sonsthagen_alaskaRussia
Entity_Type_Definition:
Table with sample identifying information, collection data, and allele scores (in base pairs) for ten autosomal microsatellite loci (Aph8, Aph16, Cmo7, Cmo9, Hhi5, Sfi10, Smo4, Smo6, Smo8, Smo12). Presented in a Comma Separated Value (CSV) formatted table.
Entity_Type_Definition_Source: Author defined
Attribute:
Attribute_Label: Sample_ID
Attribute_Definition:
USGS Alaska Science Center, Molecular Ecology Laboratory (MEL) alphanumeric label that uniquely identifies each sample.
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Unrepresentable_Domain:
Unique identifier; an alphanumeric code for each individual sample in this dataset.
Attribute:
Attribute_Label: Band_ID
Attribute_Definition:
Unique field identification code, used to mark individual birds in the field. Such as a coded plastic neck collar, tarsal flag, the final 5 digits of the metal leg band issued by the USGS Bird Banding Lab, or codes from another bird banding authority. Blank cells indicate that the bird was not banded.
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Unrepresentable_Domain: Unique field identification marker code.
Attribute:
Attribute_Label: Nest_ID
Attribute_Definition:
Unique alpha-numeric code to identify each nest from which feathers were collected.
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Unrepresentable_Domain:
Unique identifier; an alpha-numeric code to identify each nest from which feathers were collected.
Attribute:
Attribute_Label: Sample_Type
Attribute_Definition: Type of tissue from which sample DNA as collected
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: Blood
Enumerated_Domain_Value_Definition: Whole blood sample taken from a live captured bird.
Enumerated_Domain_Value_Definition_Source: Author defined
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: Feather
Enumerated_Domain_Value_Definition: Feather sample taken from a nest.
Enumerated_Domain_Value_Definition_Source: Author defined
Attribute:
Attribute_Label: Sample_location
Attribute_Definition: Place names of sample collection sites.
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: Yukon-Kuskokwim Delta
Enumerated_Domain_Value_Definition:
Samples collected on or around the Yukon-Kuskokwim River Delta, Alaska (approx 61.3N, 165.6W)
Enumerated_Domain_Value_Definition_Source: Geographic Names Information System (GNIS)
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: Utqiagvik
Enumerated_Domain_Value_Definition:
Samples collected in or around Utqiagvik, Alaska (approx 71.3N, 156.6W)
Enumerated_Domain_Value_Definition_Source: Geographic Names Information System (GNIS)
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: Colville River
Enumerated_Domain_Value_Definition:
Samples collected on or around the Colville River Delta, Alaska (approx 70.4N, 150.9W)
Enumerated_Domain_Value_Definition_Source: Geographic Names Information System (GNIS)
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: Prudhoe Bay
Enumerated_Domain_Value_Definition:
Samples collected in or around Prudhoe Bay, Alaska (approx 70.3N, 148.5W)
Enumerated_Domain_Value_Definition_Source: Geographic Names Information System (GNIS)
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: Chaun Delta
Enumerated_Domain_Value_Definition:
Samples collected on or around the Chaun River Delta, Russia (approx 68.8N, 170.5E)
Enumerated_Domain_Value_Definition_Source: NGIA GeoNames
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: Indigirka Delta
Enumerated_Domain_Value_Definition:
Samples collected on or around the Indigirka River Delta, Russia (approx 71.4N, 150.7E)
Enumerated_Domain_Value_Definition_Source: NGIA GeoNames
Attribute:
Attribute_Label: Collection_Year
Attribute_Definition: Year that sample was collected in the field.
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Range_Domain:
Range_Domain_Minimum: 1994
Range_Domain_Maximum: 2012
Attribute_Units_of_Measure: Year (YYYY)
Attribute:
Attribute_Label: CR_accession
Attribute_Definition:
GenBank Accession Number - unique identifier for the mitochondrial DNA control region (CR) nucleotide sequence, assigned by the National Center for Biotechnology Information (NCBI). Blank cells indicate that sequence data from this locus were not collected for this sample.
Attribute_Definition_Source: NCBI GenBank
Attribute_Domain_Values:
Unrepresentable_Domain:
Unique identifier: GenBank accession number assigned by NCBI. This number may be used to retrieve detailed sample and sequence information from https://www.ncbi.nlm.nih.gov/
Attribute:
Attribute_Label: Aph8_allele1 through Smo12_allele2
Attribute_Definition:
These 20 columns represent 10 autosomal microsatellite loci. Each microsatellite locus genotype is represented by two adjacent columns (i.e., allele1 and allele2) each containing one allele score. Blank cells indicate data were not collected for the sample.
Locus names and order of presentation: Aph8, Aph16, Cmo7, Cmo9, Hhi5, Sfi10, Smo4, Smo6, Smo8, Smo12
These microsatellite data cannot be combined with data generated outside the Alaska Science Center without molecular size calibration between laboratories.
Details and full citations for microsatellite loci are available at https://doi.org/10.5066/P9GJEX8Y
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Unrepresentable_Domain: Allele scores (in base pairs) for each microsatellite locus.
Distribution_Information:
Distributor:
Contact_Information:
Contact_Organization_Primary:
Contact_Organization: U.S. Geological Survey, Alaska Science Center
Contact_Address:
Address_Type: mailing and physical
Address: 4210 University Dr.
City: Anchorage
State_or_Province: Alaska
Postal_Code: 99508
Country: USA
Contact_Voice_Telephone: 907-786-7000
Contact_Electronic_Mail_Address: ascweb@usgs.gov
Resource_Description:
The USGS Alaska Science Center is the authoritative source and distributor of these data.
Distribution_Liability:
Unless otherwise stated, all data, metadata and related materials are considered to satisfy the quality standards relative to the purpose for which the data were collected. Although these data and associated metadata have been reviewed for accuracy and completeness and approved for release by the U.S. Geological Survey, no warranty expressed or implied is made regarding the display or utility of the data for other purposes or on all computer systems, nor shall the act of distribution constitute any such warranty. Any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the U.S. Government.
Standard_Order_Process:
Digital_Form:
Digital_Transfer_Information:
Format_Name: Zip
Format_Version_Number: 19.0
Format_Information_Content:
Zip file containing the data in CSV file format and FGDC metadata in XML and HTML formats.
File_Decompression_Technique: Winzip legacy compression applied
Digital_Transfer_Option:
Online_Option:
Computer_Contact_Information:
Network_Address:
Network_Resource_Name: https://doi.org/10.5066/P9F8DV8O
Fees: None
Metadata_Reference_Information:
Metadata_Date: 20201125
Metadata_Contact:
Contact_Information:
Contact_Organization_Primary:
Contact_Organization: U.S. Geological Survey, Alaska Science Center
Contact_Address:
Address_Type: mailing and physical
Address: 4210 University Drive
City: Anchorage
State_or_Province: Alaska
Postal_Code: 99508
Country: USA
Contact_Voice_Telephone: 907-786-7000
Contact_Electronic_Mail_Address: ascweb@usgs.gov
Metadata_Standard_Name:
FGDC Biological Data Profile of the Content Standard for Digital Geospatial Metadata
Metadata_Standard_Version: FGDC-STD-001.1-1999

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