Plasmodium Parasite Infection Data from Mosquitoes Collected in South-central Alaska, 2016

Metadata:

Identification_Information:
Citation:
Citation_Information:
Originator: Smith, M.M. (ORCID: 0000-0002-2259-5135)
Publication_Date: 20190614
Title:
Plasmodium Parasite Infection Data from Mosquitoes Collected in South-central Alaska, 2016
Geospatial_Data_Presentation_Form: tabular digital data
Publication_Information:
Publication_Place: Anchorage, Alaska
Publisher: U.S. Geological Survey, Alaska Science Center
Other_Citation_Details:
Smith, M.M. 2019. Plasmodium parasite infection data from mosquitoes collected in south-central Alaska, 2016. US Geological Survey data release, https://doi.org/10.5066/P9J4ZOJR
Online_Linkage: https://doi.org/10.5066/P9J4ZOJR
Larger_Work_Citation:
Citation_Information:
Originator: U.S. Geological Survey, Alaska Science Center
Publication_Date: 2015
Title:
Wildlife disease in a changing Arctic: pathogens and parasites at the marine-terrestrial interface
Geospatial_Data_Presentation_Form: website
Series_Information:
Series_Name: Alaska Science Portal
Issue_Identification: 412
Publication_Information:
Publication_Place: Anchorage, Alaska (USA)
Publisher: U.S. Geological Survey, Alaska Science Center
Other_Citation_Details:
This is a link to the broader USGS Alaska Science Center research project supported by these data. Users will find a description of the research project and links to associated reports, publications, and data products.
Online_Linkage: https://alaska.usgs.gov/portal/project.php?project_id=412
Description:
Abstract:
This data set includes two tables associated with a study of Plasmodium parasite infection in mosquitoes collected in south-central Alaska during the summer of 2016. The first table contains data regarding mosquito collection from three sites using CO2 baited CDC light traps. Mosquitoes were sorted and pooled according to collection date, genus, and location and subsequently screened for Plasmodium parasite infection using molecular methods. The second table contains results of Plasmodium parasite screening of pooled mosquito abdomen and head/thorax segments using PCR. Parasite genetic data is provided as GenBank accession numbers for any samples that tested positive for Plasmodium infection.
Purpose:
These data provide the basis to: 1) determine the possible mosquito vectors of Plasmodium parasites in south-central Alaska, and 2) to estimate the infection rate of Plasmodium in these mosquitoes and provide some of the first genetic information on Plasmodium lineages isolated from arthropod vectors from this region of Alaska.
Time_Period_of_Content:
Time_Period_Information:
Range_of_Dates/Times:
Beginning_Date: 20160508
Ending_Date: 20160808
Currentness_Reference: observed
Status:
Progress: Complete
Maintenance_and_Update_Frequency: None planned
Spatial_Domain:
Description_of_Geographic_Extent:
Mosquitoes were collected from three locations (Campbell Creek: 61.164, -149.777; Eagle River: 61.234, -149.271; Mirror Lake: 61.428, -149.425) in or near the city of Anchorage, Alaska, USA
Bounding_Coordinates:
West_Bounding_Coordinate: -149.777
East_Bounding_Coordinate: -149.271
North_Bounding_Coordinate: 61.428
South_Bounding_Coordinate: 61.164
Keywords:
Theme:
Theme_Keyword_Thesaurus: USGS Metadata Identifier
Theme_Keyword: USGS:ASC212
Theme:
Theme_Keyword_Thesaurus: ISO 19115 Topic Category
Theme_Keyword: biota
Theme:
Theme_Keyword_Thesaurus: NASA GCMD Earth Science Thesaurus
Theme_Keyword: Biological Classification
Theme_Keyword: Animals/Invertebrates
Theme_Keyword: Arthropods
Theme_Keyword: Hexapods
Theme_Keyword: Insects
Theme_Keyword: Biosphere
Theme_Keyword: Ecological Dynamics
Theme_Keyword: Species/Population Interactions
Theme_Keyword: Parasitism
Theme:
Theme_Keyword_Thesaurus: USGS CSA Biocomplexity Thesaurus
Theme_Keyword: Insects
Theme_Keyword: Diseases
Theme_Keyword: Disease reservoirs
Theme_Keyword: Disease detection
Theme_Keyword: Host-parasite interactions
Theme_Keyword: Parasitic diseases
Theme_Keyword: Vector borne diseases
Theme_Keyword: Malaria
Theme_Keyword: Protozoa
Theme:
Theme_Keyword_Thesaurus: USGS Thesaurus
Theme_Keyword: Sciences
Theme_Keyword: Life sciences
Theme_Keyword: Parasitology
Theme_Keyword: Genetics
Theme_Keyword: Polymerase chain reaction
Theme_Keyword: DNA Sequencing
Theme_Keyword: Health and Disease
Theme_Keyword: Disease vectors
Theme:
Theme_Keyword_Thesaurus: NIH MeSH Thesaurus
Theme_Keyword: Natural science disciplines
Theme_Keyword: Biological science disciplines
Theme_Keyword: Biology
Theme_Keyword: Genetics
Theme_Keyword: Parasitology
Theme_Keyword: Pathologic processes
Theme_Keyword: Disease
Theme:
Theme_Keyword_Thesaurus: none
Theme_Keyword: Blood Parasites
Place:
Place_Keyword_Thesaurus: USGS Geographic Names Information System
Place_Keyword: State of Alaska
Place_Keyword: Anchorage
Place_Keyword: Eagle River
Taxonomy:
Keywords/Taxon:
Taxonomic_Keyword_Thesaurus: None
Taxonomic_Keywords: Multiple species
Taxonomic_Keywords: Mosquito
Taxonomic_Keywords: Aedes
Taxonomic_Keywords: Culiseta
Taxonomic_Keywords: Protists
Taxonomic_Keywords: Hematozoa
Taxonomic_Keywords: Plasmodium
Taxonomic_System:
Classification_System/Authority:
Classification_System_Citation:
Citation_Information:
Originator: Integrated Taxonomic Information System
Publication_Date: Unknown
Title: Integrated Taxonomic Information System
Geospatial_Data_Presentation_Form: database
Series_Information:
Series_Name: ITIS
Issue_Identification: TSN 126234, 126429
Publication_Information:
Publication_Place: online
Publisher: ITIS-North America
Other_Citation_Details:
Taxonomy details for mosquito genera were retrieved March 14, 2019, from the Integrated Taxonomic Information System on-line database: http://www.itis.gov
Online_Linkage: https://www.itis.gov
Classification_System_Modifications: None
Classification_System/Authority:
Classification_System_Citation:
Citation_Information:
Originator: NCBI Taxonomy database
Publication_Date: Unknown
Title: National Center for Biotechnology Information Taxonomy Database
Geospatial_Data_Presentation_Form: database
Publication_Information:
Publication_Place: online
Publisher: National Center for Biotechnology Information
Other_Citation_Details:
Taxonomy details for Hematozoa were retrieved March 14, 2019, from the National Center for Biotechnology Information (NCBI) Taxonomy database on-line
Online_Linkage: https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi
Classification_System_Modifications: None
Taxonomic_Procedures:
Mosquito genera were identified by skilled observer based on appearance morphological keys. No vouchers were collected. Plasmodium were identified by nested PCR protocol and by a trained observer who examined thin blood smears stained with Geimsa at 1000x magnification.
Taxonomic_Completeness:
Taxonomy is complete for all samples. No vouchers were collected.
Taxonomic_Classification:
Taxon_Rank_Name: Life
Taxon_Rank_Value: Life
Taxonomic_Classification:
Taxon_Rank_Name: Kingdom
Taxon_Rank_Value: Animalia
Taxonomic_Classification:
Taxon_Rank_Name: Subkingdom
Taxon_Rank_Value: Bilateria
Taxonomic_Classification:
Taxon_Rank_Name: Infrakingdom
Taxon_Rank_Value: Protostomia
Taxonomic_Classification:
Taxon_Rank_Name: Superphylum
Taxon_Rank_Value: Ecdysozoa
Taxonomic_Classification:
Taxon_Rank_Name: Phylum
Taxon_Rank_Value: Arthropoda
Taxonomic_Classification:
Taxon_Rank_Name: Subphylum
Taxon_Rank_Value: Hexapoda
Taxonomic_Classification:
Taxon_Rank_Name: Class
Taxon_Rank_Value: Insecta
Taxonomic_Classification:
Taxon_Rank_Name: Subclass
Taxon_Rank_Value: Pterygota
Taxonomic_Classification:
Taxon_Rank_Name: Infraclass
Taxon_Rank_Value: Neoptera
Taxonomic_Classification:
Taxon_Rank_Name: Superorder
Taxon_Rank_Value: Holometabola
Taxonomic_Classification:
Taxon_Rank_Name: Order
Taxon_Rank_Value: Diptera
Taxonomic_Classification:
Taxon_Rank_Name: Suborder
Taxon_Rank_Value: Nematocera
Taxonomic_Classification:
Taxon_Rank_Name: Infraorder
Taxon_Rank_Value: Culicomorpha
Taxonomic_Classification:
Taxon_Rank_Name: Family
Taxon_Rank_Value: Culicidae
Applicable_Common_Name: Mosquito
Taxonomic_Classification:
Taxon_Rank_Name: Subfamily
Taxon_Rank_Value: Culicinae
Taxonomic_Classification:
Taxon_Rank_Name: Tribe
Taxon_Rank_Value: Culicini
Taxonomic_Classification:
Taxon_Rank_Name: Genus
Taxon_Rank_Value: Aedes
Applicable_Common_Name: TSN: 126234
Taxonomic_Classification:
Taxon_Rank_Name: Genus
Taxon_Rank_Value: Culiseta
Applicable_Common_Name: TSN: 126429
Taxonomic_Classification:
Taxon_Rank_Name: no rank
Taxon_Rank_Value: Alveolata
Taxonomic_Classification:
Taxon_Rank_Name: Phylum
Taxon_Rank_Value: Apicomplexa
Taxonomic_Classification:
Taxon_Rank_Name: Class
Taxon_Rank_Value: Aconoidasida
Taxonomic_Classification:
Taxon_Rank_Name: Order
Taxon_Rank_Value: Haemosporida
Applicable_Common_Name: hematozoa
Applicable_Common_Name: avian blood parasites
Taxonomic_Classification:
Taxon_Rank_Name: Family
Taxon_Rank_Value: Plasmodiidae
Taxonomic_Classification:
Taxon_Rank_Name: Genus
Taxon_Rank_Value: Plasmodium
Access_Constraints: None
Use_Constraints:
It is requested that the authors and the USGS Alaska Science Center be cited for any subsequent publications referenced to this dataset.
Point_of_Contact:
Contact_Information:
Contact_Organization_Primary:
Contact_Organization: U.S. Geological Survey, Alaska Science Center
Contact_Address:
Address_Type: mailing and physical
Address: 4210 University Drive
City: Anchorage
State_or_Province: Alaska
Postal_Code: 99508
Country: USA
Contact_Voice_Telephone: 907-786-7000
Contact_Electronic_Mail_Address: ascweb@usgs.gov
Cross_Reference:
Citation_Information:
Originator: Smith, M.M. (ORCID: 0000-0002-2259-5135)
Originator: Van Hemert, C. (ORCID: 0000-0002-6858-7165)
Originator: Handel, C.M. (ORCID: 0000-0002-0267-7408)
Publication_Date: 2019
Title:
Evidence of Culiseta mosquitoes as vectors for Plasmodium parasites in Alaska
Geospatial_Data_Presentation_Form: journal article
Series_Information:
Series_Name: Journal of Vector Ecology
Issue_Identification: 44(1):68-75
Publication_Information:
Publication_Place: online
Publisher: Society for Vector Ecology
Other_Citation_Details:
Smith, M.M., Van Hemert, C., Handel, C.M. 2019. Evidence of Culiseta mosquitoes as vectors for Plasmodium parasites in Alaska. Journal of Vector Ecology. 44(1):68-75 doi:10.1111/jvec.12330
Online_Linkage: https://doi.org/10.1111/jvec.12330
Data_Quality_Information:
Attribute_Accuracy:
Attribute_Accuracy_Report:
Collection sites, sampling dates, and taxa were all cross-checked and verified to match field data. Individual extraction numbers, genus, and pool size were verified to be correct according to laboratory notes. Sterile techniques were used throughout all molecular protocols, including positive controls for DNA extractions and negative controls included in all nested-PCR reactions. All assigned GenBank accession numbers were cross-checked for accuracy.
Logical_Consistency_Report:
Attribute values fall within expected ranges and data were proofed for the presence of duplication and omission.
Completeness_Report:
No data were omitted and there are no missing data. NA's in cells indicate that no Plasmodium DNA was detected in pools and thus no GenBank accession numbers were assigned.
Positional_Accuracy:
Horizontal_Positional_Accuracy:
Horizontal_Positional_Accuracy_Report:
Positions collected with Garmin hand held GPS with average accuracy to within 3 m.
Lineage:
Methodology:
Methodology_Type: Field
Methodology_Description:
Mosquitoes were collected from three locations (Campbell creek, Eagle River, and Mirror Lake) near Anchorage, Alaska, USA during the summer of 2016. Modified CDC model 512 miniature light traps were assembled at each location and opened for two 24-h periods per week from 8 May to 8 August. Traps were affixed to stands approximately 1.5 m tall and fitted with kill-jar assemblies containing 100% ethanol and supplemental CO2 systems to aid in mosquito attraction. Kill-jars were collected at the end of each 24-h period and mosquitoes were kept in ethanol until subsequent dissection and DNA extraction.
Methodology:
Methodology_Type: Lab
Methodology_Description:
Mosquitoes were identified to genus using morphological keys (Aquatic Biology Associates Inc, Corvallis, OR, USA). Further identification to species level was not possible due to discoloration from the ethanol used to preserve specimens. Identified mosquitoes were sorted by genus, sampling location, and sampling period prior to dissection. Samples were combined into pools of 1-25 individuals of the same genus and sampling period. Sterile techniques were used to separate mosquito abdomen and head/thorax segments.
Methodology:
Methodology_Type: Lab
Methodology_Description:
Genomic DNA was extracted from both abdomen and head/thorax pools of each sample using the DNeasy blood and tissue kit (Qiagen, Valencia, CA, USA) and a modified method described by Plichart et al. (2006). All DNA extractions were subjected to a PCR positive control which amplified a region of the cytochrome oxidase I gene (COI) prior to Plasmodium screening. All extractions that proved positive via COI positive control were screened for Plasmodium infection in triplicate using nested-PCR methods (Hellgren et al. 2004). Amplicons were visualized on 1% agarose gels stained with Gel Red (Biotium, Hayward, CA, USA).
Methodology:
Methodology_Type: Lab
Methodology_Description:
Samples that produced amplicons when visualized on agarose gels were subsequently sequenced to verify the presence of Plasmodium DNA. Products were treated with Exo-SapIT (USB Inc., Cleveland, OH, U.S.A.) at a 3:7 dilution and sent to Functional Biosciences (MGE Innovation Center, Madison, WI, USA) for sequencing. A 479 bp fragment of Plasmodium mitochondrial DNA cytochrome b gene was bidirectionally sequenced using Big Dye Terminator v3.1 mix and analyzed on an ABI 3730xl automated DNA sequencer (Applied Biosystems, Foster City, CA, U.S.A.)
Methodology:
Methodology_Type: Lab
Methodology_Description:
A subset of thin blood smears from resident birds at our study sites were screened using light microscopy to identify Plasmodium morphospecies present in the area. Smears were fixed with absolute methanol, stained with 10% Giemsa, and examined under 1000X magnification. Plasmodium morphospecies observed were identified using taxonomic keys following Valkiūnas (2005).
Source_Information:
Source_Citation:
Citation_Information:
Originator: Plichart, C.
Originator: Sechan, Y.
Originator: Davies, N.
Originator: Legrand, A
Publication_Date: 2006
Title:
PCR and dissection as tools to monitor filarial infection of Aedes polynesiensis mosquitoes in French Polynesia
Geospatial_Data_Presentation_Form: journal article
Series_Information:
Series_Name: Filaria Journal
Issue_Identification: 2
Publication_Information:
Publication_Place: online
Publisher: Biomedcentral
Other_Citation_Details:
Plichart, C., Y. Sechan, N. Davies, and A. M. Legrand. 2006. PCR and dissection as tools to monitor filarial infection of Aedes polynesiensis mosquitoes in French Polynesia. Filaria J. 5: 1–9. doi:10.1186/1475-2883-5-2
Online_Linkage: hhttps://doi.org/10.1186/1475-2883-5-2
Type_of_Source_Media: journal article
Source_Time_Period_of_Content:
Time_Period_Information:
Single_Date/Time:
Calendar_Date: 2006
Source_Currentness_Reference: publication date
Source_Citation_Abbreviation: Plichart et al. 2006
Source_Contribution: Reference for extracting genomic DNA from mosquitoes
Source_Information:
Source_Citation:
Citation_Information:
Originator: Hellgren, O.
Originator: Waldenstrom, J.
Originator: Bensch, S.
Publication_Date: 2004
Title:
A new PCR assay for simultaneous studies of Leucocytozoon, Plasmodium, and Haemoproteus from avian blood
Geospatial_Data_Presentation_Form: journal article
Series_Information:
Series_Name: Journal of Parasitology
Issue_Identification: 90
Publication_Information:
Publication_Place: online
Publisher: American Society of Parasitologists
Other_Citation_Details:
Hellgren, O., J. Waldenstrom, S. Bensch. 2004. A new PCR assay for simultaneous studies of Leucocytozoon, Plasmodium, and Haemoproteus from avian blood. J. of Parasitology, 90(4):797-802 doi:10.1645/GE-184R1
Online_Linkage: https://doi.org/10.1645/GE-184R1
Type_of_Source_Media: journal article
Source_Time_Period_of_Content:
Time_Period_Information:
Single_Date/Time:
Calendar_Date: 2004
Source_Currentness_Reference: publication date
Source_Citation_Abbreviation: Hellgren et al. 2004
Source_Contribution:
Reference for detecting haemosporidian DNA in mosquito body segments
Source_Information:
Source_Citation:
Citation_Information:
Originator: Valkiūnas, G.
Publication_Date: 2005
Title: Avian Malaria Parasites and Other Haemosporidia
Geospatial_Data_Presentation_Form: book
Publication_Information:
Publication_Place: New York
Publisher: CRC Press
Other_Citation_Details:
Valkiūnas, G. 2005. Avian Malaria Parasites and Other Haemosporidia. CRC Press, NY. 947 pp.
Type_of_Source_Media: book
Source_Time_Period_of_Content:
Time_Period_Information:
Single_Date/Time:
Calendar_Date: 2005
Source_Currentness_Reference: publication date
Source_Citation_Abbreviation: Valkiūnas 2005
Source_Contribution: Reference for identifying haemosporidia
Source_Information:
Source_Citation:
Citation_Information:
Originator: National Center for Biotechnology Information (NCBI) GenBank
Publication_Date: Unknown
Title: Reference database of known Hematozoa DNA sequences, NCBI
Geospatial_Data_Presentation_Form: application/service
Online_Linkage:
Type_of_Source_Media: digital database file
Source_Time_Period_of_Content:
Time_Period_Information:
Single_Date/Time:
Calendar_Date: 2019
Source_Currentness_Reference: observed
Source_Citation_Abbreviation: NCBI nucleotide BLAST
Source_Contribution:
DNA sequences obtained in the current study were queried against all known hematozoa sequences in National Center for Biotechnology Information (NCBI) GenBank using the Basic Local Alignment Search Tool (BLAST) and determined to be positive if there was >90% consensus with one or more entries in GenBank
Process_Step:
Process_Description: Field notes were transcribed into electronic spreadsheets.
Process_Date: Unknown
Process_Step:
Process_Description:
Sequencher version 5.1 (Gene Codes Corp., Ann Arbor, MI) was used to examine and align all sequence data. International Union of Pure and Applied Chemistry (IUPAC) ambiguity codes were assigned to base pairs with uncertain or multiple nucleic acid identity.
Process_Date: Unknown
Process_Step:
Process_Description:
Genetic sequences were assigned to genera (Plasmodium) using the nucleotide BLAST (Basic Local Alignment Search Tool) function available through the National Center for Biotechnology Information (NCBI). Assignment was based on the top NCBI BLAST result with a minimum identity score of at least 90%.
Process_Date: Unknown
Process_Step:
Process_Description:
Blood parasite infections were confirmed when sequencing resulted in a bi-directionally aligned consensus sequence of target mtDNA that was successfully assigned to genus using the nucleotide BLAST (Basic Local Alignment Search Tool) function. Sequences that were not bi-directionally aligned and sequences with <90% identity were considered negative to reduce the possible occurrence of false positives.
Process_Date: Unknown
Spatial_Data_Organization_Information:
Indirect_Spatial_Reference:
Collection site names indicate a representative local geographic name. Each site has a representative coordinate.
Direct_Spatial_Reference_Method: Point
Spatial_Reference_Information:
Horizontal_Coordinate_System_Definition:
Geographic:
Latitude_Resolution: 0.001
Longitude_Resolution: 0.001
Geographic_Coordinate_Units: Decimal degrees
Geodetic_Model:
Horizontal_Datum_Name: World Geodetic System of 1984
Ellipsoid_Name: World Geodetic System of 1984
Semi-major_Axis: 6378137
Denominator_of_Flattening_Ratio: 298.257223563
Entity_and_Attribute_Information:
Detailed_Description:
Entity_Type:
Entity_Type_Label: mosquito_plasmodiumSamples_southCentralAlaska_smith_2016
Entity_Type_Definition:
Mosquito collection data associated with this data release. Presented in a Comma Separated Value (CSV) formatted table.
Entity_Type_Definition_Source: Author defined
Attribute:
Attribute_Label: Collection Site
Attribute_Definition:
Names of three locations where modified CDC miniature light traps were deployed to collect mosquitoes.
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: Campbell Creek Science Center
Enumerated_Domain_Value_Definition:
Campbell Creek Science Center, Anchorage, Alaska (Latitude 61.164N, Longitude 149.777W; WGS84)
Enumerated_Domain_Value_Definition_Source: Author defined
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: Eagle River Nature Center
Enumerated_Domain_Value_Definition:
Eagle River Nature Center, Eagle River, Alaska (Latitude 61.234N, Longitude 149.271W; WGS84)
Enumerated_Domain_Value_Definition_Source: Author defined
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: Mirror Lake Middle School
Enumerated_Domain_Value_Definition:
Mirror Lake Middle School, Chugiak, Alaska (Latitude 61.428N, Longitude 149.425W; WGS84)
Enumerated_Domain_Value_Definition_Source: Author defined
Attribute:
Attribute_Label: Open Date
Attribute_Definition:
Date corresponding to each sample when each trap was opened for a specific 24-h period.
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Range_Domain:
Range_Domain_Minimum: 5/8/2016
Range_Domain_Maximum: 8/11/2016
Attribute_Units_of_Measure: date (MM/DD/YYYY)
Attribute:
Attribute_Label: Close Date
Attribute_Definition:
Date corresponding to each sample when each trap was closed after a specific 24-h period.
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Range_Domain:
Range_Domain_Minimum: 5/9/2016
Range_Domain_Maximum: 8/12/2016
Attribute_Units_of_Measure: date (MM/DD/YYYY)
Attribute:
Attribute_Label: Sample_ID
Attribute_Definition:
Unique identifier for each sorted sample of mosquitoes collected from collection sites on a specific date. Corresponds to the Sample_ID attribute in the other table included with this data package.
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Unrepresentable_Domain:
Unique identifier: Composed of a four letter code for corresponding to the collection site, followed by a sequential sample number for each date. Duplicate numbers indicate samples from the same location and date but of different extraction pools.
Attribute:
Attribute_Label: Taxon
Attribute_Definition: Genus of mosquitoes sorted from each sample
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: Culiseta
Enumerated_Domain_Value_Definition: Culiseta spp.; unknown species of Culiseta mosquitoes
Enumerated_Domain_Value_Definition_Source: Author defined
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: Aedes
Enumerated_Domain_Value_Definition: Aedes spp.; unknown species of Aedes mosquitoes
Enumerated_Domain_Value_Definition_Source: Author defined
Attribute:
Attribute_Label: Abundance
Attribute_Definition: Number of mosquitoes making up each sample.
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Range_Domain:
Range_Domain_Minimum: 1
Range_Domain_Maximum: 710
Detailed_Description:
Entity_Type:
Entity_Type_Label: mosquito_plasmodiumResults_southCentralAlaska_smith_2016
Entity_Type_Definition:
DNA extraction and Plasmodium infection data from mosquitoes sampled in Alaska, 2016. Presented in a Comma Separated Value (CSV) formatted table.
Entity_Type_Definition_Source: Author defined
Attribute:
Attribute_Label: Sample_ID
Attribute_Definition:
Unique identifier for each sorted sample of mosquitoes collected from collection sites on a specific date. Corresponds to the Sample_ID attribute in the other table included with this data package.
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Unrepresentable_Domain:
Unique identifier: Composed of a four letter code for corresponding to the collection site, followed by a sequential sample number for each date. Duplicate numbers indicate samples from the same location and date but different extraction pools.
Attribute:
Attribute_Label: Extraction Number
Attribute_Definition:
Unique number assigned to each individual DNA extraction of abdomen and head/thorax mosquito segments
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Unrepresentable_Domain:
Unique sample identifier: composed of a two letter code (VS) followed by a sequential extraction number (001-243).
Attribute:
Attribute_Label: Location
Attribute_Definition:
Four letter abbreviation for any of three collection sites where mosquitoes were collected
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: MLMS
Enumerated_Domain_Value_Definition: Mirror Lake Middle School collection site
Enumerated_Domain_Value_Definition_Source: Author defined
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: ERNC
Enumerated_Domain_Value_Definition: Eagle River Nature Center collection site
Enumerated_Domain_Value_Definition_Source: Author defined
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: CCSC
Enumerated_Domain_Value_Definition: Campbell Creek Science Center collection site
Enumerated_Domain_Value_Definition_Source: Author defined
Attribute:
Attribute_Label: Open date
Attribute_Definition:
Date when each trap was opened for a specific 24-h trapping period
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Range_Domain:
Range_Domain_Minimum: 5/8/2016
Range_Domain_Maximum: 8/11/2016
Attribute_Units_of_Measure: date (MM/DD/YYYY)
Attribute:
Attribute_Label: Close date
Attribute_Definition:
Date when each trap was closed at the end of each 24-h trapping period and samples were collected from kill-jar assemblies
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Range_Domain:
Range_Domain_Minimum: 5/9/2016
Range_Domain_Maximum: 8/12/2016
Attribute_Units_of_Measure: date (MM/DD/YYYY)
Attribute:
Attribute_Label: Genus
Attribute_Definition:
Mosquito genus that all individuals included in each dissection pool belonged to
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: Culiseta
Enumerated_Domain_Value_Definition: Culiseta spp.; unkown species of Culiseta mosquitoes
Enumerated_Domain_Value_Definition_Source: Author defined
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: Aedes
Enumerated_Domain_Value_Definition: Aedes spp.; unkown species of Aedes mosquitoes
Enumerated_Domain_Value_Definition_Source: Author defined
Attribute:
Attribute_Label: Pool Size
Attribute_Definition:
Size of each pooled extraction ranging from 1 to 25 individual mosquitoes
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Range_Domain:
Range_Domain_Minimum: 1
Range_Domain_Maximum: 25
Attribute:
Attribute_Label: Abdomen result
Attribute_Definition:
Results from nested-PCR screening of mosquito abdomen DNA extractions for the presence of Plasmodium parasite mtDNA
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: Neg
Enumerated_Domain_Value_Definition:
Indicates that results were negative for the presence of Plasmodium mtDNA on all three nested-PCR replicate reactions
Enumerated_Domain_Value_Definition_Source: Author defined
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: Pos
Enumerated_Domain_Value_Definition:
Indicates that any of the three replicate nested-PCR reactions successfully amplified Plasmodium mtDNA
Enumerated_Domain_Value_Definition_Source: Author defined
Attribute:
Attribute_Label: Head_Thorax result
Attribute_Definition:
Results from nested-PCR screening of mosquito head/thorax DNA extractions for the presence of Plasmodium parasite mtDNA
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: Neg
Enumerated_Domain_Value_Definition:
Indicates that results were negative for the presence of Plasmodium mtDNA on all three nested-PCR replicate reactions
Enumerated_Domain_Value_Definition_Source: Author defined
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: Pos
Enumerated_Domain_Value_Definition:
Indicates that any of the three replicate nested-PCR reactions amplified Plasmodium mtDNA
Enumerated_Domain_Value_Definition_Source: Author defined
Attribute:
Attribute_Label: GenBank Accession
Attribute_Definition:
GenBank accession number for Plasmodium cytochrome b (cyt b) mitochondrial DNA (mtDNA) target fragment following a positive result from the first replicate of the nested-PCR. NA values signify negative results from all nested-PCR reactions.
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Unrepresentable_Domain:
Unique identifier: GenBank accession number assigned by National Center for Biotechnology Information. This number may be used to retrieve detailed sample and sequence information from https://www.ncbi.nlm.nih.gov/
Distribution_Information:
Distributor:
Contact_Information:
Contact_Organization_Primary:
Contact_Organization: U.S. Geological Survey, Alaska Science Center
Contact_Address:
Address_Type: Mailing and Physical
Address: 4210 University Drive
City: Anchorage
State_or_Province: Alaska
Postal_Code: 99508
Country: USA
Contact_Voice_Telephone: 907-786-7000
Contact_Electronic_Mail_Address: ascweb@usgs.gov
Resource_Description: Authoritative source for access to these data.
Distribution_Liability:
Unless otherwise stated, all data, metadata and related materials are considered to satisfy the quality standards relative to the purpose for which the data were collected. Although these data and associated metadata have been reviewed for accuracy and completeness and approved for release by the U.S. Geological Survey (USGS), no warranty expressed or implied is made regarding the display or utility of the data on any other system or for general or scientific purposes, nor shall the act of distribution constitute any such warranty.
Standard_Order_Process:
Digital_Form:
Digital_Transfer_Information:
Format_Name: Zip
Format_Version_Number: 19.0
Format_Information_Content:
Zip file containing the data in CSV file format and FGDC metadata in XML and HTML formats.
File_Decompression_Technique: Winzip legacy compression applied
Digital_Transfer_Option:
Online_Option:
Computer_Contact_Information:
Network_Address:
Network_Resource_Name: https://doi.org/10.5066/P9J4ZOJR
Fees: None
Metadata_Reference_Information:
Metadata_Date: 20201125
Metadata_Contact:
Contact_Information:
Contact_Organization_Primary:
Contact_Organization: U.S. Geological Survey, Alaska Science Center
Contact_Address:
Address_Type: mailing and physical
Address: 4210 University Drive
City: Anchorage
State_or_Province: Alaska
Postal_Code: 99508
Country: USA
Contact_Voice_Telephone: 907-786-7000
Contact_Electronic_Mail_Address: ascweb@usgs.gov
Metadata_Standard_Name:
FGDC Biological Data Profile of the Content Standard for Digital Geospatial Metadata
Metadata_Standard_Version: FGDC-STD-001.1-1999

Generated by mp version 2.9.50 on Fri Jun 14 13:09:20 2019