Influenza A Virus Data from Migratory Birds, Izembek National Wildlife Refuge, Alaska

Metadata:

Identification_Information:
Citation:
Citation_Information:
Originator: Reeves, A.B. (ORCID: 0000-0002-7526-0726)
Publication_Date: 20181115
Title:
Influenza A Virus Data from Migratory Birds, Izembek National Wildlife Refuge, Alaska
Geospatial_Data_Presentation_Form: spreadsheet
Series_Information:
Series_Name:
Influenza A Virus Data from Migratory Birds, Izembek National Wildlife Refuge, Alaska
Issue_Identification: ver 2.0, November 2018
Publication_Information:
Publication_Place: Anchorage, AK
Publisher: U.S. Geological Survey, Alaska Science Center
Other_Citation_Details:
Suggested citation: Reeves, A. B., 2018, Influenza A Virus Data from Migratory Birds, Izembek National Wildlife Refuge, Alaska (ver. 2.0, November 2018): U.S. Geological Survey data release, https://doi.org/10.5066/F7JD4W2W
Online_Linkage: https://doi.org/10.5066/F7JD4W2W
Larger_Work_Citation:
Citation_Information:
Originator: Flint, P.L. (ORCID: 0000-0002-8758-6993)
Originator: Meixell, B.W. (ORCID: 0000-0002-6738-0349)
Originator: Pearce, J.M. (ORCID: 0000-0002-8503-5485)
Originator: Ramey, A.M. (ORCID: 0000-0002-3601-8400)
Originator: Reeves, A.B. (ORCID: 0000-0002-7526-0726)
Originator: Van Hemert, C.R. (ORCID: 0000-0002-6858-7165)
Publication_Date: 2011
Title:
Examining the role of migratory birds the movement of avian pathogens between and within continents
Geospatial_Data_Presentation_Form: website
Publication_Information:
Publication_Place: Anchorage, Alaska
Publisher: U.S. Geological Survey, Alaska Science Center
Online_Linkage: https://alaska.usgs.gov/portal/project.php?project_id=321
Description:
Abstract:
Data set containing avian influenza sampling information for late summer and early autumn waterfowl and gulls within and around the Izembek National Wildlife Refuge (NWR), Alaska, 2011-2016. Data contains species, age, sex, collection data and location of sampled migratory birds. Laboratory specific data used to identify presence and absence of influenza A viruses (IAVs) from collected samples are included.
Purpose:
The purpose of this project is to sample migratory birds in western Alaska on the Izembek NWR to determine the presence of influenza A viruses and the role of migratory birds in virus dispersal and maintenance. Specifically, we aim to: (1) evaluate which species and sampling methods are useful for recovery of IAVs at Izembek NWR during autumn, (2) characterize viral subtypes detected in each year and by species to evaluate trends of occurrence (3) describe genetic diversity for IAVs isolated from wild birds samples collected, and assess how diversity at this site relates to other regions in North America and East Asia, and (4) evaluate how detection of genetic evidence for intercontinental viral exchange relates to species sampled. Our results will inform future research and surveillance activities at Izembek NWR, provide further inference as to how information for viruses in this region relates to national and international IAV surveillance efforts, and highlight ways by which sampling efficiencies may be identified through critical evaluation of IAV research and surveillance programs.
Time_Period_of_Content:
Time_Period_Information:
Range_of_Dates/Times:
Beginning_Date: 201109
Ending_Date: 201610
Currentness_Reference: ground condition
Status:
Progress: In work
Maintenance_and_Update_Frequency: As needed
Spatial_Domain:
Description_of_Geographic_Extent:
Study area is a southwestern region of the Alaska Peninsula, specifically the areas within and around Izembek National Wildlife Refuge.
Bounding_Coordinates:
West_Bounding_Coordinate: -163.2
East_Bounding_Coordinate: -162.5
North_Bounding_Coordinate: 55.5
South_Bounding_Coordinate: 54.9
Keywords:
Theme:
Theme_Keyword_Thesaurus: ISO 19115 Topic Category
Theme_Keyword: biota
Theme:
Theme_Keyword_Thesaurus: NASA GCMD Earth Science Keywords Thesaurus
Theme_Keyword: Earth Science
Theme_Keyword: Biological Classification
Theme_Keyword: Animals/Vertebrates
Theme_Keyword: Birds
Theme_Keyword: Waterfowl
Theme_Keyword: Viruses
Theme:
Theme_Keyword_Thesaurus: USGS CSA Biocomplexity Thesaurus
Theme_Keyword: Waterfowl
Theme_Keyword: Aquatic animals
Theme:
Theme_Keyword_Thesaurus: USGS Thesaurus
Theme_Keyword: Health and disease
Theme_Keyword: Wildlife disease
Theme_Keyword: Avian influenza
Place:
Place_Keyword_Thesaurus: USGS Geographic Names Information System
Place_Keyword: State of Alaska
Place_Keyword: Alaska Peninsula
Place_Keyword: Izembek National Wildlife Refuge
Place_Keyword: Izembek Lagoon
Taxonomy:
Keywords/Taxon:
Taxonomic_Keyword_Thesaurus: None
Taxonomic_Keywords: Single species
Taxonomic_Keywords: Animals
Taxonomic_Keywords: Vertebrates
Taxonomic_Keywords: Bird
Taxonomic_System:
Classification_System/Authority:
Classification_System_Citation:
Citation_Information:
Originator: Integrated Taxonomic Information System
Publication_Date: Unknown
Title: Integrated Taxonomic Information System
Geospatial_Data_Presentation_Form: database
Publication_Information:
Publication_Place: online
Publisher: ITIS-North America
Other_Citation_Details:
Taxonomic details for bird species were retrieved on October 27, 2017, from the Integrated Taxonomic Information System on-line database http://www.itis.gov
Online_Linkage: http://www.itis.gov
Classification_System_Modifications:
ITIS currently lists Emperor Geese in the genus Chen. However, the most current taxonomic designation by the American Ornithological Society's (AOU) Check-list of North American Birds lists places Emperor Geese in the genus Anser. Here, we follow the current AOU designation as described in: Chesser, R.T., Burns, K.J., Cicero, C., Dunn, J.L., Kratter, A.W., Lovette, I.J., Rasmussen, P.C., Remsen, J.V.,Jr., Rising, J.D., Stotz, D.F., Winker, K. 2017. Fifty-eighth supplement to the American Ornithological Society's Check-list of North American Birds. The Auk:134(3)751-773. doi:10.1642/AUK-17-72.1.
Classification_System/Authority:
Classification_System_Citation:
Citation_Information:
Originator: International Committee on Taxonomy of Viruses (ICTV)
Publication_Date: Unknown
Title: International Committee on Taxonomy of Viruses
Geospatial_Data_Presentation_Form: database
Publication_Information:
Publication_Place: online
Publisher: International Committee on Taxonomy of Viruses
Other_Citation_Details:
Taxonomic details for influenza A virus were retrieved October 31, 2018, from the International Committee on Taxonomy of Viruses online database https://talk.ictvonline.org/
Online_Linkage: https://talk.ictvonline.org/
Taxonomic_Procedures:
Avian species were identified by skilled observers in the field based on general appearance of feathers and plumage, behavior, and vocalization. Influenza A viruses were identified by the successful amplification of an RNA influenza A matrix gene-target using real-time reverse transcriptase polymerase chain reaction (rRT-PCR).
Taxonomic_Classification:
Taxon_Rank_Name: Kingdom
Taxon_Rank_Value: Animalia
Applicable_Common_Name: Animal
Applicable_Common_Name: animaux
Applicable_Common_Name: animals
Taxonomic_Classification:
Taxon_Rank_Name: Subkingdom
Taxon_Rank_Value: Bilateria
Taxonomic_Classification:
Taxon_Rank_Name: Infrakingdom
Taxon_Rank_Value: Deuterostomia
Taxonomic_Classification:
Taxon_Rank_Name: Phylum
Taxon_Rank_Value: Chordata
Applicable_Common_Name: cordés
Applicable_Common_Name: cordado
Applicable_Common_Name: chordates
Taxonomic_Classification:
Taxon_Rank_Name: Subphylum
Taxon_Rank_Value: Vertebrata
Applicable_Common_Name: vertebrado
Applicable_Common_Name: vertébrés
Applicable_Common_Name: vertebrates
Taxonomic_Classification:
Taxon_Rank_Name: Infraphylum
Taxon_Rank_Value: Gnathostomata
Taxonomic_Classification:
Taxon_Rank_Name: Superclass
Taxon_Rank_Value: Tetrapoda
Taxonomic_Classification:
Taxon_Rank_Name: Class
Taxon_Rank_Value: Aves
Applicable_Common_Name: Birds
Applicable_Common_Name: oiseaux
Taxonomic_Classification:
Taxon_Rank_Name: Order
Taxon_Rank_Value: Anseriformes
Applicable_Common_Name: Waterfowl
Taxonomic_Classification:
Taxon_Rank_Name: Family
Taxon_Rank_Value: Anatidae
Applicable_Common_Name: Ducks
Applicable_Common_Name: Geese
Applicable_Common_Name: Swans
Applicable_Common_Name: canards
Applicable_Common_Name: cygnes
Applicable_Common_Name: oies
Taxonomic_Classification:
Taxon_Rank_Name: Subfamily
Taxon_Rank_Value: Anserinae
Taxonomic_Classification:
Taxon_Rank_Name: Genus
Taxon_Rank_Value: Branta
Taxonomic_Classification:
Taxon_Rank_Name: Species
Taxon_Rank_Value: Branta bernicla
Applicable_Common_Name: Brant Goose
Applicable_Common_Name: Brent Goose
Applicable_Common_Name: Ganso de collar
Applicable_Common_Name: Brant
Applicable_Common_Name: bernache cravant
Taxonomic_Classification:
Taxon_Rank_Name: Subspecies
Taxon_Rank_Value: Branta bernicla nigricans
Applicable_Common_Name: Black Brant
Taxonomic_Classification:
Taxon_Rank_Name: Species
Taxon_Rank_Value: Branta hutchinsii
Applicable_Common_Name: Cackling Goose
Taxonomic_Classification:
Taxon_Rank_Name: Genus
Taxon_Rank_Value: Anser
Taxonomic_Classification:
Taxon_Rank_Name: Species
Taxon_Rank_Value: Anser albifrons
Applicable_Common_Name: Ganso careto-mayor
Applicable_Common_Name: Greater White-fronted Goose
Applicable_Common_Name: oie rieuse
Taxonomic_Classification:
Taxon_Rank_Name: Species
Taxon_Rank_Value: Anser canagica
Applicable_Common_Name: Emperor Goose
Taxonomic_Classification:
Taxon_Rank_Name: Subfamily
Taxon_Rank_Value: Anatinae
Taxonomic_Classification:
Taxon_Rank_Name: Genus
Taxon_Rank_Value: Anas
Applicable_Common_Name: Dabbling Ducks
Taxonomic_Classification:
Taxon_Rank_Name: Species
Taxon_Rank_Value: Anas platyrhynchos
Applicable_Common_Name: Pato de collar
Applicable_Common_Name: Mallard
Applicable_Common_Name: canard colvert
Taxonomic_Classification:
Taxon_Rank_Name: Species
Taxon_Rank_Value: Anas strepera
Applicable_Common_Name: Pato friso
Applicable_Common_Name: Gadwall
Applicable_Common_Name: canard chipeau
Taxonomic_Classification:
Taxon_Rank_Name: Species
Taxon_Rank_Value: Anas acuta
Applicable_Common_Name: Pato golondrino
Applicable_Common_Name: Northern Pintail
Applicable_Common_Name: canard pilet
Taxonomic_Classification:
Taxon_Rank_Name: Species
Taxon_Rank_Value: Anas crecca
Applicable_Common_Name: Cerceta ala verde
Applicable_Common_Name: Green-winged Teal
Applicable_Common_Name: sarcelle d'hiver
Taxonomic_Classification:
Taxon_Rank_Name: Subspecies
Taxon_Rank_Value: Anas crecca carolinensis
Applicable_Common_Name: American Green-winged Teal
Taxonomic_Classification:
Taxon_Rank_Name: Species
Taxon_Rank_Value: Anas americana
Applicable_Common_Name: Pato chalcuán
Applicable_Common_Name: American Wigeon
Applicable_Common_Name: canard d'Amérique
Taxonomic_Classification:
Taxon_Rank_Name: Species
Taxon_Rank_Value: Anas clypeata
Applicable_Common_Name: Pato cucharón-norteño
Applicable_Common_Name: Northern Shoveler
Applicable_Common_Name: canard souchet
Taxonomic_Classification:
Taxon_Rank_Name: Species
Taxon_Rank_Value: Anas penelope
Applicable_Common_Name: Pato silbón
Applicable_Common_Name: Eurasian Wigeon
Applicable_Common_Name: canard siffleur
Taxonomic_Classification:
Taxon_Rank_Name: Genus
Taxon_Rank_Value: Aythya
Applicable_Common_Name: Diving Ducks
Taxonomic_Classification:
Taxon_Rank_Name: Species
Taxon_Rank_Value: Aythya marila
Applicable_Common_Name: Pato boludo-mayor
Applicable_Common_Name: Greater Scaup
Applicable_Common_Name: fuligule milouinan
Taxonomic_Classification:
Taxon_Rank_Name: Species
Taxon_Rank_Value: Aythya affinis
Applicable_Common_Name: Pato boludo-menor
Applicable_Common_Name: Lesser Scaup
Applicable_Common_Name: petit fuligule
Taxonomic_Classification:
Taxon_Rank_Name: Species
Taxon_Rank_Value: Aythya collaris
Applicable_Common_Name: Pato pico anillado
Applicable_Common_Name: Ring-necked Duck
Applicable_Common_Name: fuligule à collier
Taxonomic_Classification:
Taxon_Rank_Name: Species
Taxon_Rank_Value: Aythya valisineria
Applicable_Common_Name: Pato coacoxtle
Applicable_Common_Name: Canvasback
Applicable_Common_Name: fuligule à dos blanc
Taxonomic_Classification:
Taxon_Rank_Name: Genus
Taxon_Rank_Value: Bucephala
Taxonomic_Classification:
Taxon_Rank_Name: Species
Taxon_Rank_Value: Bucephala clangula
Applicable_Common_Name: Pato chillón
Applicable_Common_Name: Common Goldeneye
Applicable_Common_Name: garrot à oeil d'or
Taxonomic_Classification:
Taxon_Rank_Name: Species
Taxon_Rank_Value: Bucephala albeola
Applicable_Common_Name: Pato monja
Applicable_Common_Name: Bufflehead
Applicable_Common_Name: petit garrot
Taxonomic_Classification:
Taxon_Rank_Name: Genus
Taxon_Rank_Value: Clangula
Taxonomic_Classification:
Taxon_Rank_Name: Species
Taxon_Rank_Value: Clangula hyemalis
Applicable_Common_Name: Long-tailed Duck
Applicable_Common_Name: Pato cola larga
Applicable_Common_Name: Oldsquaw
Applicable_Common_Name: harelde kakawi
Taxonomic_Classification:
Taxon_Rank_Name: Genus
Taxon_Rank_Value: Melanitta
Applicable_Common_Name: Scoters
Taxonomic_Classification:
Taxon_Rank_Name: Species
Taxon_Rank_Value: Melanitta fusca
Applicable_Common_Name: Velvet Scoter
Applicable_Common_Name: Negreta ala blanca
Applicable_Common_Name: macreuse brune
Applicable_Common_Name: White-winged Scoter
Taxonomic_Classification:
Taxon_Rank_Name: Subspecies
Taxon_Rank_Value: Melanitta fusca deglandi
Taxonomic_Classification:
Taxon_Rank_Name: Species
Taxon_Rank_Value: Melanitta nigra
Applicable_Common_Name: Negreta negra
Applicable_Common_Name: Black Scoter
Applicable_Common_Name: macreuse noire
Taxonomic_Classification:
Taxon_Rank_Name: Subspecies
Taxon_Rank_Value: Melanitta nigra americana
Taxonomic_Classification:
Taxon_Rank_Name: Genus
Taxon_Rank_Value: Mergus
Taxonomic_Classification:
Taxon_Rank_Name: Species
Taxon_Rank_Value: Mergus serrator
Applicable_Common_Name: Mergo copetón
Applicable_Common_Name: Red-breasted Merganser
Applicable_Common_Name: harle huppé
Taxonomic_Classification:
Taxon_Rank_Name: Genus
Taxon_Rank_Value: Somateria
Taxonomic_Classification:
Taxon_Rank_Name: Species
Taxon_Rank_Value: Somateria spectabilis
Applicable_Common_Name: King Eider
Applicable_Common_Name: eider à tête grise
Taxonomic_Classification:
Taxon_Rank_Name: Species
Taxon_Rank_Value: Somateria mollissima
Applicable_Common_Name: Common Eider
Applicable_Common_Name: eider à duvet
Taxonomic_Classification:
Taxon_Rank_Name: Genus
Taxon_Rank_Value: Histrionicus
Taxonomic_Classification:
Taxon_Rank_Name: Species
Taxon_Rank_Value: Histrionicus histrionicus
Applicable_Common_Name: Pato arlequín
Applicable_Common_Name: Harlequin Duck
Applicable_Common_Name: arlequin plongeur
Taxonomic_Classification:
Taxon_Rank_Name: Order
Taxon_Rank_Value: Charadriiformes
Taxonomic_Classification:
Taxon_Rank_Name: Family
Taxon_Rank_Value: Laridae
Applicable_Common_Name: Gulls
Applicable_Common_Name: Terns
Taxonomic_Classification:
Taxon_Rank_Name: Genus
Taxon_Rank_Value: Larus
Applicable_Common_Name: Gulls
Taxonomic_Classification:
Taxon_Rank_Name: Species
Taxon_Rank_Value: Larus glaucescens
Applicable_Common_Name: Glaucous-winged Gull
Applicable_Common_Name: Gaviota ala glauca
Applicable_Common_Name: Goéland à ailes grises
Taxonomic_Classification:
Taxon_Rank_Name: Kingdom
Taxon_Rank_Value: viruses
Taxonomic_Classification:
Taxon_Rank_Name: Phylum
Taxon_Rank_Value: Negarnaviricota
Taxonomic_Classification:
Taxon_Rank_Name: Subphylum
Taxon_Rank_Value: Polyploviricotina
Taxonomic_Classification:
Taxon_Rank_Name: Class
Taxon_Rank_Value: Insthoviricetes
Taxonomic_Classification:
Taxon_Rank_Name: Order
Taxon_Rank_Value: Articulavirales
Taxonomic_Classification:
Taxon_Rank_Name: Family
Taxon_Rank_Value: Orthomyxoviridae
Taxonomic_Classification:
Taxon_Rank_Name: Genus
Taxon_Rank_Value: Alphainfluenzavirus
Taxonomic_Classification:
Taxon_Rank_Name: Species
Taxon_Rank_Value: Influenza A virus
Access_Constraints: None
Use_Constraints:
It is requested that the authors and the USGS Alaska Science Center be cited for any subsequent publications referenced to this dataset. It is strongly recommended that careful attention be paid to the contents of the metadata file associated with these data to evaluate data set limitations, restrictions or intended use.
Point_of_Contact:
Contact_Information:
Contact_Organization_Primary:
Contact_Organization: U.S. Geological Survey, Alaska Science Center
Contact_Address:
Address_Type: Mailing and Physical
Address: 4210 University Dr.
City: Anchorage
State_or_Province: Alaska
Postal_Code: 99508
Country: USA
Contact_Voice_Telephone: 907-786-7000
Contact_Electronic_Mail_Address: ascweb@usgs.gov
Data_Set_Credit:
U.S. Geological Survey, National Wildlife Health Center, Madison, WI. University of Georgia, Southeastern Cooperative Wildlife Disease Study, Athens, GA.
Cross_Reference:
Citation_Information:
Originator: Reeves, A.B.
Originator: Hall, J.S.
Originator: Poulson, R.L.
Originator: Donnelly, T.F.
Originator: Stallknecht, D.E.
Originator: Ramey, A.M.
Publication_Date: 2018
Title:
Influenza A virus recovery, diversity, and intercontinental exchange: A multi-year assessment of wild bird sampling at Izembek National Wildlife Refuge, Alaska
Geospatial_Data_Presentation_Form: journal article
Series_Information:
Series_Name: PLoS ONE
Issue_Identification: 13(4)e0195327
Publication_Information:
Publication_Place: online
Publisher: PLoS
Other_Citation_Details:
Reeves, A.B., Hall, J.S., Poulson, R.L., Donnelly, T.F., Stallknecht, D.E., Ramey, A.M. (2018) Influenza A virus recovery, diversity, and intercontinental exchange: A multi-year assessment of wild bird sampling at Izembek National Wildlife Refuge, Alaska. PLoS ONE 13(4):e0195327. doi:10.1371/journal.pone.0195327
Online_Linkage: https://doi.org/10.1371/journal.pone.0195327
Data_Quality_Information:
Attribute_Accuracy:
Attribute_Accuracy_Report:
Individual sample ID's, collection dates, sampling locations, numbers, and reported age and sex of individual samples provided by collectors were cross-checked and verified to match the accompanying worksheet. GenBank accession numbers were cross-referenced to the appropriate individual samples as recorded. Genomic RNA from viruses reported as "mixed" infections or "mixed" subtype were sequenced at least one additional time from a freshly prepared library to reduce the possibility that a "mixed" result could be attributed to contamination within the Next-Generation Sequencing pipeline.
Logical_Consistency_Report:
All values fall within expected ranges and data were checked for duplication and omission.
Completeness_Report:
Some collected samples were not included in this data set for the following reasons: there was suspected contamination, the cause of a bird’s death was unknown, or the sample was collected from an illegally harvested bird. We justify omitting data for the later two reasons because sample types (cloacal and oropharyngeal swabs) from dead and/or illegally harvested emperor geese (Anser canagica) and glaucous-winged gulls (Larus glaucescens) differed from the primary methods for collecting samples from these species (fecal samples from the environment). Collection and IAV screening data are provided for all other collected samples.
Lineage:
Methodology:
Methodology_Type: Field
Methodology_Description:
Hunter-harvested waterfowl were sampled for IAVs for birds harvested within and around Izembek National Wildlife Refuge (see bounding coordinates). Swab samples for viral screening were collected from each bird by applying a polyester-tipped applicator to the cloaca only (2011-2014) or the cloaca and orophranx/trachea (2015-2016). Paired samples were then combined in a single cryovial. Additionally, fecal deposits from emperor geese and glaucous-winged gulls were swabbed. All swabs were placed in cyrovials with chilled viral transport media supplemented with antibiotics. Samples were then placed directly in dry shippers charged with liquid nitrogen and transferred to -80˚C freezers until testing.
Methodology:
Methodology_Type: Lab
Methodology_Description:
In the laboratory, all paired swab samples were screened using real-timer reverse transcriptase polymerase chain reaction (rRT-PCR) targeting gene products for the influenza A virus matrix gene. All samples yielding Ct values < 45 for the IAV matrix gene were inoculated into specific pathogen-free embryonated chicken eggs for virus isolation. Amnio-allantoic fluids were collected after 120 hours, and tested via hemagglutination assay. RNAs extracted from hemagglutination assay positive amnio-allantoic fluids were screened for the influenza A virus matrix gene by rRT-PCR and resultant isolates were genomically sequenced
Process_Step:
Process_Description:
Nucleotide sequences from samples that were genomically sequenced were submitted to the National Center for Biotechnology Information (NCBI) GenBank website for public release http://www.ncbi.nlm.nih.gov/genbank/
Process_Date: 2017
Process_Step:
Process_Description:
'Sample data' were transcribed from hand-written field data collection sheets, and laboratory data collection spreadsheets created by staff at the U.S. Geological Survey, National Wildlife Health Center, and the University of Georgia, Southeastern Cooperative Wildlife Disease Study.
Process_Date: 2017
Process_Step:
Process_Description:
'Isolates' data were transcribed from laboratory records at the U.S. Geological Survey, Alaska Science Center.
Process_Date: 2017
Spatial_Data_Organization_Information:
Indirect_Spatial_Reference:
Southwestern region of the Alaska Peninsula within and around Izembek National Wildlife Refuge.
Entity_and_Attribute_Information:
Detailed_Description:
Entity_Type:
Entity_Type_Label: avianInfluenza_sampling_izembekNWR_reeves
Entity_Type_Definition: Table of bird samples and test results for influenza A viruses
Entity_Type_Definition_Source: Author defined.
Attribute:
Attribute_Label: Sample_ID
Attribute_Definition:
Individualized identification provided for each sample collected, starting with year of collection and then a unique sample number.
Attribute_Definition_Source: Author defined.
Attribute_Domain_Values:
Range_Domain:
Range_Domain_Minimum: 2011-0001
Range_Domain_Maximum: 2016-0949
Attribute:
Attribute_Label: Alternative_Sample_ID
Attribute_Definition:
The laboratory number is assigned by the laboratory at the University of Georgia where the original samples collected in 2015-2016 were processed. The prefix: UG indicates the samples were processed at the University of Georgia, followed by AI to indicate the samples are screened for avian influenza, lastly there is a two-character code for the year of collection (15=2015). Each sample is assigned a unique number separated by the prefix by a "-". Blank field indicate no alternative sample ID was provided.
Attribute_Definition_Source: Author defined.
Attribute_Domain_Values:
Range_Domain:
Range_Domain_Minimum: UGAI15-6066
Range_Domain_Maximum: UGAI16-5350
Attribute:
Attribute_Label: Sample_Type
Attribute_Definition:
The type of sample that was collected. All samples were collected with sterile plastic-shaft, polyester-tipped swabs and placed in viral transport media (VTM).
Attribute_Definition_Source: Author defined.
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: CL
Enumerated_Domain_Value_Definition:
Cloacal. A swab was inserted into the cloaca of a bird and preserved in VTM
Enumerated_Domain_Value_Definition_Source: Author defined.
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: OP
Enumerated_Domain_Value_Definition:
Oropharyngeal. A swab was inserted into the oropharyngeal region of a bird and preserved in VTM
Enumerated_Domain_Value_Definition_Source: Author defined.
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: CL/OP
Enumerated_Domain_Value_Definition:
Cloacal and oropharyngeal. Both swabs were collected from an individual bird and inserted together in a single tube of VTM
Enumerated_Domain_Value_Definition_Source: Author defined.
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: F
Enumerated_Domain_Value_Definition:
Fecal. Feces in the environment was swabbed and preserved in VTM
Enumerated_Domain_Value_Definition_Source: Author defined.
Attribute:
Attribute_Label: Species
Attribute_Definition:
Common name of avian species sampled as identified by field technicians and or volunteers. The species names "green-winged teal" and "Canada goose" recorded on original data sheets were changed here to "American green-winged teal" and "cackling goose" respectively for consistency of reporting.
Attribute_Definition_Source: Author Defined.
Attribute_Domain_Values:
Codeset_Domain:
Codeset_Name:
AOU Check-list of North and Middle American Birds, Seventh Edition
Codeset_Source: American Ornithologists Union. http://checklist.aou.org/
Attribute:
Attribute_Label: Age
Attribute_Definition:
Age (year Class) is represented as age (year) codes specified by the USGS, Bird Banding Laboratory, Patuxent Wildlife Research Center. Age is determined by feather plumage coloration, pattern, or feather wear of specific feather tracks.
Attribute_Definition_Source:
USGS, Bird Banding Laboratory, Patuxent Wildlife Research Center, Patuxent, Maryland https://www.pwrc.usgs.gov/bbl/
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: AHY
Enumerated_Domain_Value_Definition:
After hatching year. A bird known to have hatched before the calendar year of banding; year of hatch otherwise unknown.
Enumerated_Domain_Value_Definition_Source: Author defined.
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: HY
Enumerated_Domain_Value_Definition:
Hatching year. A bird capable of sustained flight and known to have hatched during the calendar year in which it was banded.
Enumerated_Domain_Value_Definition_Source: Author defined.
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: U
Enumerated_Domain_Value_Definition:
Unknown age. Age not determined or indeterminable by methods described.
Enumerated_Domain_Value_Definition_Source: Author defined.
Attribute:
Attribute_Label: Sex
Attribute_Definition:
Sex of the sampled duck as determined by cloacal exam and plumage characteristics; M = Male, F = Female, U = unknown.
Attribute_Definition_Source: Author defined.
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: F
Enumerated_Domain_Value_Definition: Female
Enumerated_Domain_Value_Definition_Source: Author defined.
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: M
Enumerated_Domain_Value_Definition: Male
Enumerated_Domain_Value_Definition_Source: Author defined.
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: U
Enumerated_Domain_Value_Definition: Unknown
Enumerated_Domain_Value_Definition_Source: Author defined.
Attribute:
Attribute_Label: Band_Number
Attribute_Definition:
Unique 8 or 9-digit number stamped on a metal leg band issued by the U.S. Geological Survey, Bird Banding Lab, Patuxent Wildlife Research Center, Patuxent, Maryland. Bands generally begin with a 3-4 digit prefix followed by a 5 digit suffix, e.g., 1347-59381. The initial capture and banding event and associated band number are reported to the USGS Bird Banding Laboratory in the year of banding and all banding and encounter data can be obtained from the Bird Banding Laboratory. Blank fields indicate that no band was observed on the sampled bird.
Attribute_Definition_Source:
Source: USGS Bird Banding Laboratory, Patuxent Wildlife Research Center, Patuxent, Maryland https://www.pwrc.usgs.gov/bbl/
Attribute_Domain_Values:
Range_Domain:
Range_Domain_Minimum: 1076-07928
Range_Domain_Maximum: 3617-25199
Attribute:
Attribute_Label: Collection_date
Attribute_Definition:
Date of collection event (e.g. date samples were collected from hunter-harvested birds or day fecal sample was taken (mm/dd/yyyy).
Attribute_Definition_Source: Author defined.
Attribute_Domain_Values:
Range_Domain:
Range_Domain_Minimum: 9/2/2011
Range_Domain_Maximum: 10/13/2016
Attribute_Units_of_Measure: Date
Attribute:
Attribute_Label: MA_RT-PCR_Ct_Values
Attribute_Definition:
The number of cycles required for the fluorescent signal of a molecular probe for the influenza A matrix gene target to exceed background levels in a real-time reverse transcriptase polymerase chain reaction (rRT-PCR) applied to swab samples stored in viral transport media as described by Spackman et al. (2002). The rRT-PCR is run for up to 45 cycles using reaction conditions described in the previous reference.
Attribute_Definition_Source:
Author defined, with reference to: Spackman, E., Senne, D.A., Myers, T.J., Bulaga, L.L., Garber, L.P., Perdue, M.L., Lohman, K., Daum, L.T., Suarez, D.L (2002) Development of a real-time reverse transcriptase PCR assay for type A influenza virus and the avian H5 and H7 hemagglutinin subtypes. Journal of Clinical Microbiology. 40:3256–3260. doi:10.1128/jcm.40.93256-3260.2002
Attribute_Domain_Values:
Range_Domain:
Range_Domain_Minimum: 20.41
Range_Domain_Maximum: 44.93
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: No Ct
Enumerated_Domain_Value_Definition:
The sample was tested but did not exceed the background levels and therefore no Ct value was reported.
Enumerated_Domain_Value_Definition_Source: Author defined.
Attribute:
Attribute_Label: RT-PCR_Date
Attribute_Definition:
The date that the rRT-PCR was performed in mm/dd/yyyy/ format. Blank fields indicate the rRT-PCR date is unknown or was not recorded.
Attribute_Definition_Source: Author defined.
Attribute_Domain_Values:
Range_Domain:
Range_Domain_Minimum: 10/25/2011
Range_Domain_Maximum: 1/20/2016
Attribute_Units_of_Measure: Date
Attribute:
Attribute_Label: Final_HI_Result
Attribute_Definition:
The results of a hemagglutination inhibition (HI) assay also known as hemagglutination assay in which sialic acid receptor on red blood cells bind to hemaglutinin proteins. This assay confirms agglutination activity of a sample but does not confirm the presence of influenza A viruses as other viruses can also test positive for this assay. Blank fields indicate no test was performed on a given sample.
Attribute_Definition_Source: Author defined.
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: POS
Enumerated_Domain_Value_Definition: Assay result positive for agglutination of red blood cells
Enumerated_Domain_Value_Definition_Source: Author defined.
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: NEG
Enumerated_Domain_Value_Definition: Assay result negative for agglutination of red blood cells
Enumerated_Domain_Value_Definition_Source: Author defined.
Attribute:
Attribute_Label: AIVM_VI
Attribute_Definition:
The detection of the influenza A matrix gene target in RNA extracted from amnio-allantoic fluids using real-time reverse transcriptase polymerase chain reaction (rRT-PCR) as described by Fouchier et al. (2000). Only samples put into eggs have a reported result; blank fields indicate eggs were not inoculated with a given sample.
Attribute_Definition_Source:
Author defined, with reference to: Fouchier, R.A.M., Bestebroer, T.M., Herfst, S., Van Der Kemp, L., Rimmelzwaan, G.F., Osterhaus, A.D.M.E. (200) Detection of influenza A viruses from different species by PCR amplification of conserved sequences in the matrix gene. Journal of Clinical Microbiology. 38:4096–4101.
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: POS
Enumerated_Domain_Value_Definition: Assay result positive
Enumerated_Domain_Value_Definition_Source: Author defined
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: NEG
Enumerated_Domain_Value_Definition: Assay result negative
Enumerated_Domain_Value_Definition_Source: Author defined.
Detailed_Description:
Entity_Type:
Entity_Type_Label: avianInfluenza_isolates_izembekNWR_reeves
Entity_Type_Definition:
All wild bird samples that resulted in the isolation of influenza A viruses. Data pertaining to the characterization of these viruses, such as strain names, subtypes, and GenBank Accession numbers are included.
Entity_Type_Definition_Source: Author defined.
Attribute:
Attribute_Label: Sample_ID
Attribute_Definition:
Individualized identification provided for each sample collected starting with year of collection and then a unique sample number.
Attribute_Definition_Source: Author defined.
Attribute_Domain_Values:
Range_Domain:
Range_Domain_Minimum: 2011-0028
Range_Domain_Maximum: 2016-0947
Attribute:
Attribute_Label: Strain
Attribute_Definition:
Genetic Isolate. Identifier for a genetic variant of an influenza A virus, starting with the virus type (A), common name of host species the virus was isolated from, location the sample was collected, and unique sample or lab ID.
Attribute_Definition_Source: Author defined.
Attribute_Domain_Values:
Unrepresentable_Domain: Unique identifier of genetic isolate.
Attribute:
Attribute_Label: HA_Subtype
Attribute_Definition:
The Hemagglutinin subtype based on serological differences found among all influenza A viruses. If more than one subtype was identified from an individual sample it is reported under the attribute label 'Second HA Subtype.'
Attribute_Definition_Source: Author defined.
Attribute_Domain_Values:
Range_Domain:
Range_Domain_Minimum: H1
Range_Domain_Maximum: H16
Attribute:
Attribute_Label: Second_HA_Subtype
Attribute_Definition:
The second Hemagglutinin subtype detected from an individual sample based on serological differences found among all influenza A viruses. Blank cells indicate, only one subtype was identified.
Attribute_Definition_Source: Author defined.
Attribute_Domain_Values:
Range_Domain:
Range_Domain_Minimum: H3
Range_Domain_Maximum: H13
Attribute:
Attribute_Label: NA_Subtype
Attribute_Definition:
The Neuraminidase subtype based on serological differences found among all influenza A viruses. If more than one subtype was identified from an individual sample it is reported under the attribute label 'Second NA Subtype.'
Attribute_Definition_Source: Author defined.
Attribute_Domain_Values:
Range_Domain:
Range_Domain_Minimum: N1
Range_Domain_Maximum: N9
Attribute:
Attribute_Label: Second_NA_Subtype
Attribute_Definition:
The second Neuraminidase subtype detected from an individual sample based on serological differences found among all influenza A viruses. Blank cells indicate, only one subtype was identified.
Attribute_Definition_Source: Author defined.
Attribute_Domain_Values:
Range_Domain:
Range_Domain_Minimum: N2
Range_Domain_Maximum: N8
Attribute:
Attribute_Label: HA_NA_Combo
Attribute_Definition:
The combination of hemagglutinin and neuraminidase subtypes. If more than one nucleotide sequence is reported for any of the eight influenza A virus gene segments from an individual sample, the subtype combination is reported as “mixed” whether more than one of each surface glycoprotein (HA or NA) were detected or not.
Attribute_Definition_Source: Author defined.
Attribute_Domain_Values:
Range_Domain:
Range_Domain_Minimum: H1N1
Range_Domain_Maximum: H16N3
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: mixed
Enumerated_Domain_Value_Definition:
If more than one nucleotide sequence was identified for any of the eight gene segments, the subtype combination is reported as "mixed."
Enumerated_Domain_Value_Definition_Source: Author defined.
Attribute:
Attribute_Label: GenBank_Accession_PB2
Attribute_Definition:
GenBank Accession numbers for influenza A virus gene segment 1, known as PB2. An accession number is a unique identifier given to a nucleotide sequence or other biological polymer sequence (DNA, protein) when it is submitted to NCBI GenBank database. “N/A” is the reported domain value when multiple sequences from an individual sample align to the same gene segment and could not be resolved. If more than one sequence was resolved per gene segment from and individual sample, the additional accession number is reported in a separate “secondsequence” column. Genbank may be accessed at https://www.ncbi.nlm.nih.gov/genbank/
Attribute_Definition_Source: Author defined.
Attribute_Domain_Values:
Range_Domain:
Range_Domain_Minimum: KP336376
Range_Domain_Maximum: MG976689
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: N/A
Enumerated_Domain_Value_Definition: No sequence was generated or published for this gene segment.
Enumerated_Domain_Value_Definition_Source: Author defined.
Attribute:
Attribute_Label: GenBank_Accession_PB1
Attribute_Definition:
GenBank Accession numbers for influenza A virus gene segment 2, known as PB1. An accession number is a unique identifier given to a nucleotide sequence or other biological polymer sequence (DNA, protein) when it is submitted to NCBI GenBank database. “N/A” is the reported domain value when multiple sequences from an individual sample align to the same gene segment and could not be resolved. If more than one sequence was resolved per gene segment from and individual sample, the additional accession number is reported in a separate “secondsequence” column. Genbank may be accessed at https://www.ncbi.nlm.nih.gov/genbank/
Attribute_Definition_Source: Author defined.
Attribute_Domain_Values:
Range_Domain:
Range_Domain_Minimum: KP336377
Range_Domain_Maximum: MG976690
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: N/A
Enumerated_Domain_Value_Definition: No sequence was generated or published for this gene segment.
Enumerated_Domain_Value_Definition_Source: Author defined.
Attribute:
Attribute_Label: GenBank Accession_PB1_second_sequence
Attribute_Definition:
GenBank Accession numbers for influenza A virus gene segment 2, known as PB1. An accession number is a unique identifier given to a nucleotide sequence or other biological polymer sequence (DNA, protein) when it is submitted to NCBI GenBank database. “N/A” is the reported domain value when multiple sequences from an individual sample align to the same gene segment and could not be resolved. If more than one sequence was resolved per gene segment from and individual sample, the additional accession number is reported in a separate “secondsequence” column. Genbank may be accessed at https://www.ncbi.nlm.nih.gov/genbank/
Attribute_Definition_Source: Author defined.
Attribute_Domain_Values:
Range_Domain:
Range_Domain_Minimum: KY131283
Range_Domain_Maximum: KY131283
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: N/A
Enumerated_Domain_Value_Definition: No sequence was generated or published for this gene segment.
Enumerated_Domain_Value_Definition_Source: Author defined.
Attribute:
Attribute_Label: GenBank_Accession_PA
Attribute_Definition:
GenBank Accession numbers for influenza A virus gene segment 3, known as PA. An accession number is a unique identifier given to a nucleotide sequence or other biological polymer sequence (DNA, protein) when it is submitted to NCBI GenBank database. “N/A” is the reported domain value when multiple sequences from an individual sample align to the same gene segment and could not be resolved. If more than one sequence was resolved per gene segment from and individual sample, the additional accession number is reported in a separate “secondsequence” column. Genbank may be accessed at https://www.ncbi.nlm.nih.gov/genbank/
Attribute_Definition_Source: Author defined.
Attribute_Domain_Values:
Range_Domain:
Range_Domain_Minimum: KP336378
Range_Domain_Maximum: MG976691
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: N/A
Enumerated_Domain_Value_Definition: No sequence was generated or published for this gene segment.
Enumerated_Domain_Value_Definition_Source: Author defined.
Attribute:
Attribute_Label: GenBank_Accession_PA_second_sequence
Attribute_Definition:
GenBank Accession numbers for influenza A virus gene segment 3, known as PA. An accession number is a unique identifier given to a nucleotide sequence or other biological polymer sequence (DNA, protein) when it is submitted to NCBI GenBank database. “N/A” is the reported domain value when multiple sequences from an individual sample align to the same gene segment and could not be resolved. If more than one sequence was resolved per gene segment from and individual sample, the additional accession number is reported in a separate “secondsequence” column. Genbank may be accessed at https://www.ncbi.nlm.nih.gov/genbank/
Attribute_Definition_Source: Author defined.
Attribute_Domain_Values:
Range_Domain:
Range_Domain_Minimum: KY131285
Range_Domain_Maximum: KY131285
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: N/A
Enumerated_Domain_Value_Definition: No sequence was generated or published for this gene segment.
Enumerated_Domain_Value_Definition_Source: Author defined.
Attribute:
Attribute_Label: GenBank_Accession_HA
Attribute_Definition:
GenBank Accession numbers for influenza A virus gene segment 4, known as HA. An accession number is a unique identifier given to a nucleotide sequence or other biological polymer sequence (DNA, protein) when it is submitted to NCBI GenBank database. “N/A” is the reported domain value when multiple sequences from an individual sample align to the same gene segment and could not be resolved. If more than one sequence was resolved per gene segment from and individual sample, the additional accession number is reported in a separate “secondsequence” column. Genbank may be accessed at https://www.ncbi.nlm.nih.gov/genbank/
Attribute_Definition_Source: Author defined.
Attribute_Domain_Values:
Range_Domain:
Range_Domain_Minimum: KP336379
Range_Domain_Maximum: MG976692
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: N/A
Enumerated_Domain_Value_Definition: No sequence was generated or published for this gene segment.
Enumerated_Domain_Value_Definition_Source: Author defined.
Attribute:
Attribute_Label: GenBank_Accession_HA_second_sequence
Attribute_Definition:
GenBank Accession numbers for influenza A virus gene segment 4, known as HA. An accession number is a unique identifier given to a nucleotide sequence or other biological polymer sequence (DNA, protein) when it is submitted to NCBI GenBank database. “N/A” is the reported domain value when multiple sequences from an individual sample align to the same gene segment and could not be resolved. If more than one sequence was resolved per gene segment from and individual sample, the additional accession number is reported in a separate “secondsequence” column. Genbank may be accessed at https://www.ncbi.nlm.nih.gov/genbank/
Attribute_Definition_Source: Author defined.
Attribute_Domain_Values:
Range_Domain:
Range_Domain_Minimum: KT338335
Range_Domain_Maximum: KY131287
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: N/A
Enumerated_Domain_Value_Definition: No sequence was generated or published for this gene segment.
Enumerated_Domain_Value_Definition_Source: Author defined.
Attribute:
Attribute_Label: GenBank_Accession_NP
Attribute_Definition:
GenBank Accession numbers for influenza A virus gene segment 5, known as NP. An accession number is a unique identifier given to a nucleotide sequence or other biological polymer sequence (DNA, protein) when it is submitted to NCBI GenBank database. “N/A” is the reported domain value when multiple sequences from an individual sample align to the same gene segment and could not be resolved. If more than one sequence was resolved per gene segment from and individual sample, the additional accession number is reported in a separate “secondsequence” column. Genbank may be accessed at https://www.ncbi.nlm.nih.gov/genbank/
Attribute_Definition_Source: Author defined.
Attribute_Domain_Values:
Range_Domain:
Range_Domain_Minimum: KP336380
Range_Domain_Maximum: MG976693
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: N/A
Enumerated_Domain_Value_Definition: No sequence was generated or published for this gene segment.
Enumerated_Domain_Value_Definition_Source: Author defined.
Attribute:
Attribute_Label: GenBank_Accession_NP_second_sequence
Attribute_Definition:
GenBank Accession numbers for influenza A virus gene segment 5, known as NP. An accession number is a unique identifier given to a nucleotide sequence or other biological polymer sequence (DNA, protein) when it is submitted to NCBI GenBank database. “N/A” is the reported domain value when multiple sequences from an individual sample align to the same gene segment and could not be resolved. If more than one sequence was resolved per gene segment from and individual sample, the additional accession number is reported in a separate “secondsequence” column. Genbank may be accessed at https://www.ncbi.nlm.nih.gov/genbank/
Attribute_Definition_Source: Author defined.
Attribute_Domain_Values:
Range_Domain:
Range_Domain_Minimum: KY131289
Range_Domain_Maximum: KY131289
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: N/A
Enumerated_Domain_Value_Definition: No sequence was generated or published for this gene segment.
Enumerated_Domain_Value_Definition_Source: Author defined.
Attribute:
Attribute_Label: GenBank_Accession_NA
Attribute_Definition:
GenBank Accession numbers for influenza A virus gene segment 6, known as NA. An accession number is a unique identifier given to a nucleotide sequence or other biological polymer sequence (DNA, protein) when it is submitted to NCBI GenBank database. “N/A” is the reported domain value when multiple sequences from an individual sample align to the same gene segment and could not be resolved. If more than one sequence was resolved per gene segment from and individual sample, the additional accession number is reported in a separate “secondsequence” column. Genbank may be accessed at https://www.ncbi.nlm.nih.gov/genbank/
Attribute_Definition_Source: Author defined.
Attribute_Domain_Values:
Range_Domain:
Range_Domain_Minimum: KP336381
Range_Domain_Maximum: MG976694
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: N/A
Enumerated_Domain_Value_Definition: No sequence was generated or published for this gene segment.
Enumerated_Domain_Value_Definition_Source: Author defined.
Attribute:
Attribute_Label: GenBank_Accession_NA_second_sequence
Attribute_Definition:
GenBank Accession numbers for influenza A virus gene segment 6, known as NA. An accession number is a unique identifier given to a nucleotide sequence or other biological polymer sequence (DNA, protein) when it is submitted to NCBI GenBank database. “N/A” is the reported domain value when multiple sequences from an individual sample align to the same gene segment and could not be resolved. If more than one sequence was resolved per gene segment from and individual sample, the additional accession number is reported in a separate “secondsequence” column. Genbank may be accessed at https://www.ncbi.nlm.nih.gov/genbank/
Attribute_Definition_Source: Author defined.
Attribute_Domain_Values:
Range_Domain:
Range_Domain_Minimum: KT338337
Range_Domain_Maximum: KY131291
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: N/A
Enumerated_Domain_Value_Definition: No sequence was generated or published for this gene segment.
Enumerated_Domain_Value_Definition_Source: Author defined.
Attribute:
Attribute_Label: GenBank_Accession_MA
Attribute_Definition:
GenBank Accession numbers for influenza A virus gene segment 7, known as MA. An accession number is a unique identifier given to a nucleotide sequence or other biological polymer sequence (DNA, protein) when it is submitted to NCBI GenBank database. “N/A” is the reported domain value when multiple sequences from an individual sample align to the same gene segment and could not be resolved. If more than one sequence was resolved per gene segment from and individual sample, the additional accession number is reported in a separate “secondsequence” column. Genbank may be accessed at https://www.ncbi.nlm.nih.gov/genbank/
Attribute_Definition_Source: Author defined.
Attribute_Domain_Values:
Range_Domain:
Range_Domain_Minimum: KP336382
Range_Domain_Maximum: MG976695
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: N/A
Enumerated_Domain_Value_Definition: No sequence was generated or published for this gene segment.
Enumerated_Domain_Value_Definition_Source: Author defined.
Attribute:
Attribute_Label: GenBank_Accession_NS
Attribute_Definition:
GenBank Accession numbers for influenza A virus gene segment 8, known as NS. An accession number is a unique identifier given to a nucleotide sequence or other biological polymer sequence (DNA, protein) when it is submitted to NCBI GenBank database. “N/A” is the reported domain value when multiple sequences from an individual sample align to the same gene segment and could not be resolved. If more than one sequence was resolved per gene segment from and individual sample, the additional accession number is reported in a separate “secondsequence” column. Genbank may be accessed at https://www.ncbi.nlm.nih.gov/genbank/
Attribute_Definition_Source: Author defined.
Attribute_Domain_Values:
Range_Domain:
Range_Domain_Minimum: KP336383
Range_Domain_Maximum: MG976696
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: N/A
Enumerated_Domain_Value_Definition: No sequence was generated or published for this gene segment.
Enumerated_Domain_Value_Definition_Source: Author defined.
Attribute:
Attribute_Label: GenBank_Accession_NS_second_sequence
Attribute_Definition:
GenBank Accession numbers for influenza A virus gene segment 8, known as NS. An accession number is a unique identifier given to a nucleotide sequence or other biological polymer sequence (DNA, protein) when it is submitted to NCBI GenBank database. “N/A” is the reported domain value when multiple sequences from an individual sample align to the same gene segment and could not be resolved. If more than one sequence was resolved per gene segment from and individual sample, the additional accession number is reported in a separate “secondsequence” column. Genbank may be accessed at https://www.ncbi.nlm.nih.gov/genbank/
Attribute_Definition_Source: Author defined.
Attribute_Domain_Values:
Range_Domain:
Range_Domain_Minimum: KT338379
Range_Domain_Maximum: KY131268
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: N/A
Enumerated_Domain_Value_Definition: No sequence was generated or published for this gene segment.
Enumerated_Domain_Value_Definition_Source: Author defined.
Distribution_Information:
Distributor:
Contact_Information:
Contact_Organization_Primary:
Contact_Organization: U.S. Geological Survey, Alaska Science Center
Contact_Address:
Address_Type: Mailing and Physical
Address: 4210 University Drive
City: Anchorage
State_or_Province: AK
Postal_Code: 99508
Country: USA
Contact_Voice_Telephone: 907-786-7000
Contact_Electronic_Mail_Address: ascweb@usgs.gov
Distribution_Liability:
Unless otherwise stated, all data, metadata and related materials are considered to satisfy the quality standards relative to the purpose for which the data were collected. Although these data and associated metadata have been reviewed for accuracy and completeness and approved for release by the U.S. Geological Survey (USGS), no warranty expressed or implied is made regarding the display or utility of the data on any other system or for general or scientific purposes, nor shall the act of distribution constitute any such warranty.
Standard_Order_Process:
Digital_Form:
Digital_Transfer_Information:
Format_Name: Zip
Format_Version_Number: 19.0
Format_Information_Content:
Zip file containing the data in CSV file format and FGDC metadata in XML and HTML formats.
File_Decompression_Technique: Winzip legacy compression applied
Digital_Transfer_Option:
Online_Option:
Computer_Contact_Information:
Network_Address:
Network_Resource_Name: https://doi.org/10.5066/F7JD4W2W
Fees: None
Metadata_Reference_Information:
Metadata_Date: 20181017
Metadata_Contact:
Contact_Information:
Contact_Organization_Primary:
Contact_Organization: U.S. Geological Survey, Alaska Science Center
Contact_Address:
Address_Type: Mailing and Physical
Address: 4210 University Drive
City: Anchorage
State_or_Province: AK
Postal_Code: 99508
Country: USA
Contact_Voice_Telephone: 907-786-7000
Contact_Electronic_Mail_Address: ascweb@usgs.gov
Metadata_Standard_Name:
FGDC Biological Data Profile of the Content Standard for Digital Geospatial Metadata
Metadata_Standard_Version: FGDC-STD-001.1-1999

Generated by mp version 2.9.49 on Wed Nov 14 20:02:04 2018