Microsatellite Genetic Data for Sea Sandwort (Honckenya peploides) and Merckia (Wilhelmsia physodes), Alaska, 2009-2016

Metadata:

Identification_Information:
Citation:
Citation_Information:
Originator: Gravley, M.C. (ORCID: 0000-0002-4947-0236)
Originator: Pierson, B.J. (ORCID: 0000-0001-8233-874X)
Originator: Sage, G.K. (ORCID: 0000-0003-1431-2286)
Originator: Talbot, S.L. (ORCID: 0000-0002-3312-7214)
Publication_Date: 201805
Title:
Microsatellite Genetic Data for Sea Sandwort (Honckenya peploides) and Merckia (Wilhelmsia physodes), Alaska, 2009-2016
Geospatial_Data_Presentation_Form: spreadsheet
Publication_Information:
Publication_Place: Anchorage, AK
Publisher: U.S. Geological Survey, Alaska Science Center
Other_Citation_Details:
Suggested citation: Gravley, M.C., Pierson, B.J., Sage, G.K., and Talbot, S.L., 2018, Microsatellite Genetic Data for Sea Sandwort (Honckenya peploides) and Merckia (Wilhelmsia physodes), Alaska, 2009-2016: U.S. Geological Survey data release, https://doi.org/10.5066/F7BC3XRP.
Online_Linkage: https://doi.org/10.5066/F7BC3XRP
Larger_Work_Citation:
Citation_Information:
Originator: Talbot, S.L. (ORCID: 0000-0002-3312-7214)
Publication_Date: 2000
Title:
Developing and Applying Molecular Tools to Natural Resource Problems in Alaska
Geospatial_Data_Presentation_Form: web site
Publication_Information:
Publication_Place: Anchorage, AK
Publisher: U.S. Geological Survey, Alaska Science Center
Online_Linkage: https://alaska.usgs.gov/portal/project.php?project_id=378
Description:
Abstract:
This dataset contains sampling information and genetic data of 12 novel microsatellite loci developed and polymorphic in the Sea Sandwort (Honckenya peploides) and screened in Merckia (Wilhelmsia physodes) from Alaska. The allele sizes for the 12 microsatellite loci developed in this study are included for two populations of Sea Sandwort (Fossil Beach, Kodiak and Egg Bay, Atka) and one population of Merckia (upper Coleen River, Brooks Range).
Purpose:
The Sea Sandwort (Honckenya peploides) is of ecological interest as a ruderal species with subdioecious reproduction. Co-dominant microsatellites are well-understood genetic markers suitable for population studies providing insight into dispersal patterns and population structure. Merckia (Wilhelmsia physodes) is considered the most closely-related species to H. peploides so microsatellites were screened in one population of Merckia as well to assess their utility in other species.
Supplemental_Information:
Individuals using these data are encouraged to review the following primary publications(s) derived from these data: Gravley, M.C., Sage, G.K., Talbot, S.L., and Carlson, M.L. (2018) Development and characterization of 12 polymorhpic microsatellite loci in the sea sandwort, Honckenya peploides (L.) Ehrh. J. Plant Res.
Time_Period_of_Content:
Time_Period_Information:
Range_of_Dates/Times:
Beginning_Date: 2015
Ending_Date: 2017
Currentness_Reference: observed
Status:
Progress: Complete
Maintenance_and_Update_Frequency: Unknown
Spatial_Domain:
Description_of_Geographic_Extent:
Clam Lagoon, Adak Island; Egg Bay, Atka Island; Casco Cove, Attu Island; Fossil Beach, Kodiak Island; upper Coleen River, Brooks Range; Alaska, USA
Bounding_Coordinates:
West_Bounding_Coordinate: 173.16443
East_Bounding_Coordinate: -142.374234
North_Bounding_Coordinate: 68.494933
South_Bounding_Coordinate: 51.93295
Keywords:
Theme:
Theme_Keyword_Thesaurus: USGS Metadata Identifier
Theme_Keyword: USGS:ASC180
Theme:
Theme_Keyword_Thesaurus: NASA GCMD Earth Science Keywords Thesaurus
Theme_Keyword: Biological Classification
Theme_Keyword: Plants
Theme_Keyword: Angiosperms (Flowering Plants)
Theme_Keyword: Dicots
Theme_Keyword: Biosphere
Theme_Keyword: Vegetation
Theme_Keyword: Indigenous Vegetation
Theme:
Theme_Keyword_Thesaurus: USGS CSA Biocomplexity Thesaurus
Theme_Keyword: Genetic diversity
Theme_Keyword: Biodiversity
Theme_Keyword: Population genetics
Theme_Keyword: Genetic variance
Theme_Keyword: Seed dispersal
Theme_Keyword: Ruderal environments
Theme_Keyword: Terrestrial environments
Theme:
Theme_Keyword_Thesaurus: ISO 19115 Topic Category
Theme_Keyword: biota
Theme_Keyword: environment
Theme:
Theme_Keyword_Thesaurus: USGS Thesaurus
Theme_Keyword: Sciences
Theme_Keyword: Life Sciences
Theme_Keyword: Genetics
Theme_Keyword: Polymerase chain reaction
Theme_Keyword: Biodiversity
Theme_Keyword: Ecosystem diversity
Theme_Keyword: Genetic diversity
Theme_Keyword: Habitat alteration
Theme_Keyword: Population dynamics
Theme_Keyword: Terrestrial ecosystems
Theme_Keyword: Biogeography
Theme_Keyword: Ecological Processes
Theme_Keyword: Coastal Ecosystems
Theme_Keyword: Vegetation
Theme_Keyword: Flowering Plants
Theme:
Theme_Keyword_Thesaurus: MeSH-National Library of Medicine
Theme_Keyword: North America
Theme_Keyword: Alaska
Theme_Keyword: Alleles
Theme_Keyword: High-Throughput Nucleotide Sequencing
Theme_Keyword: Diploidy
Theme_Keyword: Genetics, Population
Theme_Keyword: Gene Flow
Theme_Keyword: Microsatellite Repeats
Theme_Keyword: Salt-Tolerant Plants
Theme_Keyword: Desiccation
Theme_Keyword: Rhizome
Theme_Keyword: Seeds
Theme_Keyword: Oceans and Seas
Place:
Place_Keyword_Thesaurus: USGS Geographic Names Information System
Place_Keyword: Clam Lagoon
Place_Keyword: Adak Island
Place_Keyword: Egg Bay
Place_Keyword: Atka Island
Place_Keyword: Casco Cove
Place_Keyword: Attu Island
Place_Keyword: Kodiak Island
Place_Keyword: Coleen River
Place_Keyword: Brooks Range
Place:
Place_Keyword_Thesaurus: none
Place_Keyword: Fossil Beach
Taxonomy:
Keywords/Taxon:
Taxonomic_Keyword_Thesaurus: None
Taxonomic_Keywords: collection
Taxonomic_Keywords: multiple species
Taxonomic_Keywords: single species
Taxonomic_Keywords: plants
Taxonomic_Keywords: vegetation
Taxonomic_System:
Classification_System/Authority:
Classification_System_Citation:
Citation_Information:
Originator: Integrated Taxonomic Information System
Publication_Date: Unknown
Title: Integrated Taxonomic Information System
Geospatial_Data_Presentation_Form: database
Publication_Information:
Publication_Place: Online
Publisher: ITIS-North America
Other_Citation_Details:
Retrieved Honckenya peploides (TSN 19970) and Wilhelmsia physodes (TSN 20374) December 4, 2017 from the Integrated Taxonomic Information System on-line database http://www.itis.gov
Online_Linkage: Online_Linkage:
Taxonomic_Procedures:
Species were identified by skilled observers in the field based on morphology and habitat.
Taxonomic_Classification:
Taxon_Rank_Name: Kingdom
Taxon_Rank_Value: Plantae
Applicable_Common_Name: plantes
Applicable_Common_Name: Planta
Applicable_Common_Name: Vegetal
Applicable_Common_Name: plants
Taxonomic_Classification:
Taxon_Rank_Name: Subkingdom
Taxon_Rank_Value: Viridiplantae
Taxonomic_Classification:
Taxon_Rank_Name: Infrakingdom
Taxon_Rank_Value: Streptophyta
Applicable_Common_Name: land plants
Taxonomic_Classification:
Taxon_Rank_Name: Superdivision
Taxon_Rank_Value: Embryophyta
Taxonomic_Classification:
Taxon_Rank_Name: Division
Taxon_Rank_Value: Tracheophyta
Applicable_Common_Name: vascular plants
Applicable_Common_Name: tracheophytes
Taxonomic_Classification:
Taxon_Rank_Name: Subdivision
Taxon_Rank_Value: Spermatophytina
Applicable_Common_Name: spermatophytes
Applicable_Common_Name: seed plants
Applicable_Common_Name: phanérogames
Taxonomic_Classification:
Taxon_Rank_Name: Class
Taxon_Rank_Value: Magnoliopsida
Taxonomic_Classification:
Taxon_Rank_Name: Superorder
Taxon_Rank_Value: Caryophyllanae
Taxonomic_Classification:
Taxon_Rank_Name: Order
Taxon_Rank_Value: Caryophyllales
Taxonomic_Classification:
Taxon_Rank_Name: Family
Taxon_Rank_Value: Caryophyllaceae
Applicable_Common_Name: pinks
Applicable_Common_Name: cariophyllacées
Taxonomic_Classification:
Taxon_Rank_Name: Genus
Taxon_Rank_Value: Honckenya
Taxonomic_Classification:
Taxon_Rank_Name: Species
Taxon_Rank_Value: Honckenya peploides
Applicable_Common_Name: seaside sandplant
Taxonomic_Classification:
Taxon_Rank_Name: Genus
Taxon_Rank_Value: Wilhelmsia
Taxonomic_Classification:
Taxon_Rank_Name: Species
Taxon_Rank_Value: Wilhelmsia physodes
Applicable_Common_Name: merckia
Access_Constraints: None
Use_Constraints:
If you use these data in a publication, presentation, or other product, it is requested that you credit USGS and the authors of this data for their contribution to your work. Microsatellite data from this dataset cannot be combined with data generated outside the Alaska Science Center without molecular size calibration between laboratories.
Point_of_Contact:
Contact_Information:
Contact_Organization_Primary:
Contact_Organization: U.S. Geological Survey, Alaska Science Center
Contact_Address:
Address_Type: mailing and physical
Address: 4210 University Drive
City: Anchorage
State_or_Province: Alaska
Postal_Code: 99508
Country: USA
Contact_Voice_Telephone: 907-786-7000
Contact_Electronic_Mail_Address: ascweb@usgs.gov
Cross_Reference:
Citation_Information:
Originator: Gravley, M.C. (ORCID: 0000-0002-4947-0236)
Originator: Sage, G.K. (ORCID: 0000-0003-1431-2286)
Originator: Talbot, S.L. (ORCID: 0000-0002-3312-7214)
Originator: Carlson, M.L.
Publication_Date: 201804
Title:
Development and characterization of 12 polymorphic microsatellite loci in the sea sandwort, Honckenya peploides (L.) Ehrh.
Geospatial_Data_Presentation_Form: journal article
Series_Information:
Series_Name: Journal of Plant Research
Issue_Identification: April, 2018
Publication_Information:
Publication_Place: Online
Publisher: Springer Japan
Other_Citation_Details:
Gravley, M.C., Sage, G.K., Talbot, S.L. et al. J Plant Res (2018). https://doi.org/10.1007/s10265-018-1036-7
Online_Linkage: https://doi.org/10.1007/s10265-018-1036-7
Data_Quality_Information:
Attribute_Accuracy:
Attribute_Accuracy_Report:
Individual sample ID's, collection dates, sampling locations, band numbers, and reported sex of individual samples provided by collectors were cross-checked and verified to match the accompanying worksheet.
Sampling locations were verified with collectors and formally labelled and cross-referenced to the Geographic Names Information Service (GNIS) when possible.
For quality control purposes, DNA from a miniumu 20% of individuals for both Honckenya peploides and Wilhelmsia physodes were extracted and microsatellites amplified in duplicate. All duplicate characterizations of the same individual resulted in concordant data.
After analysis of R (genotypic richness) in GenClone, version 2.0 (Arnaud-Haond S, Belkhir K [2007] GENCLONE: a computer program to analyse genotypic data, test for clonality and describe spatial clonal organization. Mol Ecol Notes 7:15–17. doi: 10.1111/j.1471-8286.2006.01522.x) and GeneCap (Wilberg MJ, Dreher BP [2004] GENECAP: a program for analysis of multilocus genotype data for non-invasive sampling and capture-recapture population estimation. Mol Ecol Notes 4:783–785. doi: 10.1111/j.1471-8286.2004.00797.x), a single representative of each identical multilocus genotype was chosen randomly and labelled as the ramet while other individuals were labelled as genets (three multilocus genotypes had two individuals each, therefore only 3 individuals are labelled as ramets in the dataset).
Logical_Consistency_Report:
For microsatellite data, reported allele sizes were reviewed to confirm they conform to expected distributions (e.g. dinucleotide repeats with 1 base pair differences were reviewed on gel images with laboratory personnel, gaps in allele sizes were reviewed, and single occurrences of an allele size were scrutinized). One sample (HOPE Attu09-01) consistently exhibits three alleles at two microsatellite loci (HOPE09 and HOPE43) consistent with the occurrence of polyploidism in many plant species. Exact sampling date and locations were provided on Wilhelmsia sampling sheets. However, all the samples were placed collectively into one preservation container precluding exact date/location assignment to a specific individual. The date range of the collection effort (6/29/2016-7/6/2016) for Wilhelmsia is provided within the Field_collection_start and Field_collection_end attributes. The transect location information for Wilhelmsia is listed as “NA” (not applicable) though all samples are known to have been collected within a bounding box of 68.493 N, 68.467 S, -142.374 E, -142.457 W. Exact sampling dates were not provided with Wilhelmsia samples. The date range of the collection effort (6/29/2016-7/6/2016) is provided within the Field_collection_start and Field_collection_end attributes. Specific collection dates are known for all Honckenya samples. Therefore both the Field_collection_start and Field_collection_end attributes contain identical values.
Completeness_Report:
Data omissions for particular entities are explained in the Entity and Attribute definitions. Blank data indicates no data were collected or not provided with the sample for a particular attribute. Attribute data equaling “NP” in a cell of the data set refers to a sample that did not amplify or show any product (“no product”). Attribute data equaling “NS” indicates resulting PCR product was present, but was not scorable due to weak signal or co-amplified products indiscernible between non-specific priming vs. duplicated locus without further investigation. Two Honckenya peploides (Attu09-01 and Adak09-30) were used only in shotgun sequencing to identify and develop the microsatellites and their primers, respectively. Data for these two individuals are included here, but were not included in Gravley et al (2018).
Positional_Accuracy:
Horizontal_Positional_Accuracy:
Horizontal_Positional_Accuracy_Report:
Geospatial data were acquired from the contributing data sources. These methods were not documented in the source data.
Lineage:
Methodology:
Methodology_Type: Lab
Methodology_Description:
Novel microsatellite development followed methods as described in Sonsthagen et al (2013) with the addition of two other species in a multiplex run of HOPE Attu09-01 and single run of HOPE Adak09-30.
Methodology_Citation:
Citation_Information:
Originator: Sonsthagen, S.A. (ORCID: 0000-0001-6215-5874)
Originator: Sage, G.K. (ORCID: 0000-0003-1431-2286)
Originator: Fowler, M. (ORCID: 0000-0002-4947-0236)
Originator: Hope, A.G.
Originator: Cook, J.A. (ORCID: 0000-0003-3985-0670)
Originator: Talbot, S.L.(ORCID: 0000-0002-3312-7214)
Publication_Date: 201303
Title:
Development and characterization of 21 polymorphic microsatellite markers for the barren ground shrew, Sorex ugyunak (Mammalia: Sorcidae), through next-generation sequencing, and cross-species amplification in the masked shrew, S. cinereus
Geospatial_Data_Presentation_Form: journal article
Series_Information:
Series_Name: Conservation Genetics Resources
Issue_Identification: 2
Publication_Information:
Publication_Place: Online
Publisher: Springer Netherlands
Other_Citation_Details: DOI: 10.1007/s12686-012-9792-5
Online_Linkage: https://link.springer.com/article/10.1007%2Fs12686-012-9792-5
Methodology:
Methodology_Type: Lab
Methodology_Description:
Extraction procedures followed those described elsewhere (Muñiz-Salazar et al. 2005), with the following modifications: enzymatic lysis used proteinase K with incubation at 55 °C for 3-5 days, after incubation and addition of chloroform:isoamyl alcohol (24:1 v/v) samples were briefly vortexed at a low rpm before centrifugation, and precipitated DNA in isopropanol was incubated for 20 minutes at −80d C before centrifugation. Following DNA precipitation, a wash was performed by adding 500 µL of 70% EtOH to each sample and samples were centrifuged at 6000 rpm for 5 minutes. After decanting EtOH and allowing residual liquid to evaporate, the samples were rehydrated with 1X Tris-EDTA.
Methodology_Citation:
Citation_Information:
Originator: Muñiz-Salazar, R. (ORCID: 0000-0001-5528-7772)
Originator: Talbot, S.L. (ORCID: 0000-0002-3312-7214)
Originator: Sage, G.K. (ORCID: 0000-0003-1431-2286)
Publication_Date: 2005
Title:
Population genetic structure of annual and perennial populations of Zostera marina L. along the Pacific coast of Baja California and the Gulf of California
Geospatial_Data_Presentation_Form: journal article
Series_Information:
Series_Name: Mol Ecol
Issue_Identification: 14:711–722
Publication_Information:
Publication_Place: online
Publisher: Wiley Online Library
Online_Linkage:
Methodology:
Methodology_Type: Lab
Methodology_Description:
Polymerase chain reaction (PCR) amplification and scoring of microsatellites followed Handel et al. (2006) except the thermocycler parameters consisted of 40 cycles at 94d C for 15 seconds, 50-54d C for 15 seconds and 72d C for 30 seconds.
Methodology_Citation:
Citation_Information:
Originator: Handel, C.M. (ORCID: 0000-0002-0267-7408)
Originator: Pajot, L.M. (ORCID: 0000-0001-5704-2381)
Originator: Talbot, S.L. (ORCID: 0000-0002-3312-7214)
Originator: Sage, G.K. (ORCID: 0000-0003-1431-2286)
Publication_Date: 200611
Title:
Use of buccal swabs for sampling DNA from nestling and adult birds
Geospatial_Data_Presentation_Form: journal article
Series_Information:
Series_Name: Wildlife Society Bulletin
Issue_Identification: 34:1094-1100
Publication_Information:
Publication_Place: online
Publisher: The Wildlife Society
Other_Citation_Details: DOI: 10.2193/0091-7648(2006)34[1094:UOBSFS]2.0.CO;2
Online_Linkage:
Methodology:
Methodology_Type: Lab
Methodology_Description:
Size standards were obtained for each locus by selecting 1-4 heterozygous individuals and scoring them against a fluorescently labeled M13 sequence ladder of known size.
Source_Information:
Source_Citation:
Citation_Information:
Originator: Stephen Talbot, USFWS
Originator: Hunter Gravley, USFWS
Originator: Janet Jorgensen, USFWS
Publication_Date: Unpublished material
Title: Individual Contributors of Samples
Geospatial_Data_Presentation_Form: samples_samplingInformation
Type_of_Source_Media: plant tissue for genetic analyses
Source_Time_Period_of_Content:
Time_Period_Information:
Range_of_Dates/Times:
Beginning_Date: 2009
Ending_Date: 2016
Source_Currentness_Reference: observed
Source_Citation_Abbreviation: samples_samplingInformation
Source_Contribution:
Stephen Talbot and Hunter Gravley assisted in sample collection of Honckenya peploides.
Janet Jorgensen provided Wilhelmsia physodes samples and sampling information.
Process_Step:
Process_Description:
Next-generation sequencing reads used for microsatellite development were assembled into contigs using GS De Novo Assembler, Version 3.0 (Roche, Branford, CT). Contigs and unassembled reads were searched using Msatcommander 0.8.2 (Faircloth 2008) for microsatellites with the following motifs: >= dinucleotides, >= 6 trinucleotides, and >= 4 tetranucleotides.
Source_Used_Citation_Abbreviation:
Faircloth BC. 2008. MSATCOMMANDER: detection of microsatellite repeat arrays and automated, locus-specific primer design. Mol. Ecol. Resour. 8:92–94.
Process_Date: Unknown
Process_Step:
Process_Description:
Size standards, occupying four to six lanes across each gel, were used to assign allele sizes and determine genotypes in Gene Profiler 4.05 (Scanalytics, Inc.).
Process_Date: Unknown
Spatial_Data_Organization_Information:
Indirect_Spatial_Reference:
Sampling locations have been described by corresponding entries in the Geographic Names Information System (GNIS) when possible. One location commonly known as Fossil Beach on Kodiak Island, Alaska is formally un-named, but geographic coordinates are given in the attribute information.
Spatial_Reference_Information:
Horizontal_Coordinate_System_Definition:
Geographic:
Latitude_Resolution: 0.00001
Longitude_Resolution: 0.00001
Geographic_Coordinate_Units: Decimal degrees
Geodetic_Model:
Horizontal_Datum_Name: World Geodetic System of 1984
Ellipsoid_Name: World Geodetic System of 1984
Semi-major_Axis: 6378137
Denominator_of_Flattening_Ratio: 298.257223563
Entity_and_Attribute_Information:
Detailed_Description:
Entity_Type:
Entity_Type_Label: sandwortMerckia_microsats_alaska_gravley_2009_2016
Entity_Type_Definition: Data set
Entity_Type_Definition_Source: Author defined
Attribute:
Attribute_Label: Sample_ID
Attribute_Definition:
This is a U.S. Geological Survey, Alaska Science Center, Molecular Ecology Laboratory alphanumeric label that uniquely identifies each sample. The leading 4 letters are the first two letters of the genus and species, followed by an abbreviation of general geographic sampling location, followed by the 2 digit year of sampling, dash, and 2 digits identifying the individual sample.
Attribute_Definition_Source: Field assigned
Attribute_Domain_Values:
Unrepresentable_Domain:
This is a 13-15 digit alphanumeric combination representing a single individual in this dataset.
Attribute:
Attribute_Label: Species
Attribute_Definition: This is the genus and species for each sample.
Attribute_Definition_Source: Field assigned
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: Honckenya peploides
Enumerated_Domain_Value_Definition: This sample is from Honckenya peploides.
Enumerated_Domain_Value_Definition_Source: Author defined
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: Wilhelmsia physodes
Enumerated_Domain_Value_Definition: This sample is from Wilhelmsia physodes.
Enumerated_Domain_Value_Definition_Source: Author defined
Attribute:
Attribute_Label: Collection_location
Attribute_Definition:
This is the specific geographic area in which the sample was collected.
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: Clam Lagoon, Adak
Enumerated_Domain_Value_Definition:
This refers to the geographic location of Clam Lagoon on the island of Adak, Alaska.
Enumerated_Domain_Value_Definition_Source: Geographic Names Information System (GNIS)
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: Egg Bay, Atka
Enumerated_Domain_Value_Definition:
This refers to the geographic location of Egg Bay on the island of Atka, Alaska.
Enumerated_Domain_Value_Definition_Source: Geographic Names Information System (GNIS)
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: Casco Cove, Attu
Enumerated_Domain_Value_Definition:
This refers to the geographic location of Casco Cove on the island of Attu, Alaska.
Enumerated_Domain_Value_Definition_Source: Geographic Names Information System (GNIS)
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: Fossil Beach, Kodiak
Enumerated_Domain_Value_Definition:
This refers to the geographic location of Fossil Beach on Kodiak Island, Alaska.
Enumerated_Domain_Value_Definition_Source: Author defined
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: upper Coleen River
Enumerated_Domain_Value_Definition:
This refers to the geographic location of upper Coleen River, Alaska.
Enumerated_Domain_Value_Definition_Source: Geographic Names Information System (GNIS)
Attribute:
Attribute_Label: Field_collection_start
Attribute_Definition:
This indicates the specific date (month/day/year) the individual was collected from the field. If a less specific date is known, this represents the start date of the field collection effort (see Field _collection_end for end date of the field effort).
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Range_Domain:
Range_Domain_Minimum: 6/7/2009
Range_Domain_Maximum: 9/9/2016
Attribute_Units_of_Measure: day/month/year
Attribute:
Attribute_Label: Field_collection_end
Attribute_Definition:
This indicates the specific date (month/day/year) the individual was collected from the field. If a less specific date is known, this represents the end date of the field collection effort (see Field _collection_start for start date of the field effort).
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Range_Domain:
Range_Domain_Minimum: 6/7/2009
Range_Domain_Maximum: 9/9/2016
Attribute_Units_of_Measure: day/month/year
Attribute:
Attribute_Label: Transect_lat_start
Attribute_Definition:
Sampling occurred along a loose transect and individual samples were collected 5-20 meters (m) apart in all populations to decrease chances of collecting clones. This indicates the latitude of the start of the transect.
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Range_Domain:
Range_Domain_Minimum: 51.94084
Range_Domain_Maximum: 68.494933
Attribute_Units_of_Measure: decimal degrees
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: NA
Enumerated_Domain_Value_Definition:
The transect location information for Wilhelmsia is listed as "NA" (not applicable) though all samples are known to have been collected within a bounding box of 68.493 N, 68.467 S, -142.374 E, -142.457 W.
Enumerated_Domain_Value_Definition_Source: Author defined
Attribute:
Attribute_Label: Transect_long_start
Attribute_Definition:
Sampling occurred along a loose transect and individual samples were collected 5-20 meters (m) apart in all populations to decrease chances of collecting clones. This indicates the longitude of the start of the transect.
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Range_Domain:
Range_Domain_Minimum: 173.17508
Range_Domain_Maximum: -176.56432
Attribute_Units_of_Measure: decimal degrees
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: NA
Enumerated_Domain_Value_Definition:
The transect location information for Wilhelmsia is listed as "NA" (not applicable) though all samples are known to have been collected within a bounding box of 68.493 N, 68.467 S, -142.374 E, -142.457 W.
Enumerated_Domain_Value_Definition_Source: Author defined
Attribute:
Attribute_Label: Transect_lat_end
Attribute_Definition:
Sampling occurred along a loose transect and individual samples were collected 5-20 meters (m) apart in all populations to decrease chances of collecting clones. This indicates the latitude of the end of the transect.
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Range_Domain:
Range_Domain_Minimum: 51.93295
Range_Domain_Maximum: 68.470429
Attribute_Units_of_Measure: decimal degrees
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: NA
Enumerated_Domain_Value_Definition:
The transect location information for Wilhelmsia is listed as "NA" (not applicable) though all samples are known to have been collected within a bounding box of 68.493 N, 68.467 S, -142.374 E, -142.457 W.
Enumerated_Domain_Value_Definition_Source: Author defined
Attribute:
Attribute_Label: Transect_long_end
Attribute_Definition:
Sampling occurred along a loose transect and individual samples were collected 5-20 meters (m) apart in all populations to decrease chances of collecting clones. This indicates the longitude of the end of the transect.
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Range_Domain:
Range_Domain_Minimum: 173.16443
Range_Domain_Maximum: -176.55554
Attribute_Units_of_Measure: decimal degrees
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: NA
Enumerated_Domain_Value_Definition:
The transect location information for Wilhelmsia is listed as "NA" (not applicable) though all samples are known to have been collected within a bounding box of 68.493 N, 68.467 S, -142.374 E, -142.457 W.
Enumerated_Domain_Value_Definition_Source: Author defined
Attribute:
Attribute_Label: Clonality
Attribute_Definition:
This the clonal status of each sample. Because some plants can reproduce vegetatively (i.e. cloned [vs. sexually]), multiple samples may represent the same genetically unique individual. Here, the term ramet is used to describe a sample that is a clone of a unique individual (genet) already represented in the dataset.
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: Genet
Enumerated_Domain_Value_Definition: Unique individual
Enumerated_Domain_Value_Definition_Source: Author defined
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: Ramet (Adak09-08)
Enumerated_Domain_Value_Definition:
This represents a sample that is a clone of individual Adak09-08.
Enumerated_Domain_Value_Definition_Source: Author defined
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: Ramet (AtkaE16-11)
Enumerated_Domain_Value_Definition:
This represents a sample that is a clone of individual AtkaE16-11.
Enumerated_Domain_Value_Definition_Source: Author defined
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: Ramet (KdkF16-23)
Enumerated_Domain_Value_Definition:
This represents a sample that is a clone of individual KdkF16-23.
Enumerated_Domain_Value_Definition_Source: Author defined
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: Undetermined
Enumerated_Domain_Value_Definition:
The lack of genetic variation in markers screened for Wilhelmsia precludes labeling clonality in samples sharing multilocus genotypes.
Enumerated_Domain_Value_Definition_Source: Author defined
Attribute:
Attribute_Label: HOPE02_allele_1
Attribute_Definition:
Microsatellite allele one from the HOPE02 locus: genotype numbers were determined by scoring based on allele size relative to an M13 DNA sequence ladder and to samples established as size standards that were run on each gel. NP indicates resulting PCR product was absent or of insufficient quality to enable analysis. A blank cell indicates data were not generated at this locus for that sample.
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Range_Domain:
Range_Domain_Minimum: 130
Range_Domain_Maximum: 132
Attribute_Units_of_Measure: base pairs
Attribute:
Attribute_Label: HOPE02_allele_2
Attribute_Definition:
Microsatellite allele two from the HOPE02 locus: genotype numbers were determined by scoring based on allele size relative to an M13 DNA sequence ladder and to samples established as size standards that were run on each gel. NP indicates resulting PCR product was absent or of insufficient quality to enable analysis. A blank cell indicates data were not generated at this locus for that sample.
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Range_Domain:
Range_Domain_Minimum: 130
Range_Domain_Maximum: 132
Attribute_Units_of_Measure: base pairs
Attribute:
Attribute_Label: HOPE09_allele_1
Attribute_Definition:
Microsatellite allele one from the HOPE09 locus: genotype numbers were determined by scoring based on allele size relative to an M13 DNA sequence ladder and to samples established as size standards that were run on each gel. NP indicates resulting PCR product was absent or of insufficient quality to enable analysis. A blank cell indicates data were not generated at this locus for that sample.
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Range_Domain:
Range_Domain_Minimum: 114
Range_Domain_Maximum: 141
Attribute_Units_of_Measure: base pairs
Attribute:
Attribute_Label: HOPE09_allele_2
Attribute_Definition:
Microsatellite allele two from the HOPE09 locus: genotype numbers were determined by scoring based on allele size relative to an M13 DNA sequence ladder and to samples established as size standards that were run on each gel. NP indicates resulting PCR product was absent or of insufficient quality to enable analysis. A blank cell indicates data were not generated at this locus for that sample.
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Range_Domain:
Range_Domain_Minimum: 114
Range_Domain_Maximum: 144
Attribute_Units_of_Measure: base pairs
Attribute:
Attribute_Label: HOPE09_allele_3
Attribute_Definition:
Microsatellite allele three from the HOPE09 locus: genotype numbers were determined by scoring based on allele size relative to an M13 DNA sequence ladder and to samples established as size standards that were run on each gel. NOTE: locus HOPE09_allele_3 is presumed to be the result of a polyploid individual. A blank cell indicates a third allele was not observed at this locus for that sample.
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Range_Domain:
Range_Domain_Minimum: 141
Range_Domain_Maximum: 141
Attribute_Units_of_Measure: base pairs
Attribute:
Attribute_Label: HOPE14_allele_1
Attribute_Definition:
Microsatellite allele one from the HOPE14 locus: genotype numbers were determined by scoring based on allele size relative to an M13 DNA sequence ladder and to samples established as size standards that were run on each gel. NP indicates resulting PCR product was absent or of insufficient quality to enable analysis. A blank cell indicates data were not generated at this locus for that sample.
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Range_Domain:
Range_Domain_Minimum: 162
Range_Domain_Maximum: 164
Attribute_Units_of_Measure: base pairs
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: monomorphic
Enumerated_Domain_Value_Definition:
PCR product for this locus was not variable and was a large product beyond the size standard range established in Honckenya peploides.
Enumerated_Domain_Value_Definition_Source: Author defined
Attribute:
Attribute_Label: HOPE14_allele_2
Attribute_Definition:
Microsatellite allele two from the HOPE14 locus: genotype numbers were determined by scoring based on allele size relative to an M13 DNA sequence ladder and to samples established as size standards that were run on each gel. NP indicates resulting PCR product was absent or of insufficient quality to enable analysis. A blank cell indicates data were not generated at this locus for that sample.
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Range_Domain:
Range_Domain_Minimum: 162
Range_Domain_Maximum: 166
Attribute_Units_of_Measure: base pairs
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: monomorphic
Enumerated_Domain_Value_Definition:
PCR product for this locus was not variable and was a large product beyond the size standard range established in Honckenya peploides.
Enumerated_Domain_Value_Definition_Source: Author defined
Attribute:
Attribute_Label: HOPE24_allele_1
Attribute_Definition:
Microsatellite allele one from the HOPE24 locus: genotype numbers were determined by scoring based on allele size relative to an M13 DNA sequence ladder and to samples established as size standards that were run on each gel. NP indicates resulting PCR product was absent or of insufficient quality to enable analysis. A blank cell indicates data were not generated at this locus for that sample.
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Range_Domain:
Range_Domain_Minimum: 161
Range_Domain_Maximum: 182
Attribute_Units_of_Measure: base pairs
Attribute:
Attribute_Label: HOPE24_allele_2
Attribute_Definition:
Microsatellite allele two from the HOPE24 locus: genotype numbers were determined by scoring based on allele size relative to an M13 DNA sequence ladder and to samples established as size standards that were run on each gel. NP indicates resulting PCR product was absent or of insufficient quality to enable analysis. A blank cell indicates data were not generated at this locus for that sample.
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Range_Domain:
Range_Domain_Minimum: 161
Range_Domain_Maximum: 194
Attribute_Units_of_Measure: base pairs
Attribute:
Attribute_Label: HOPE28_allele_1
Attribute_Definition:
Microsatellite allele one from the HOPE28 locus: genotype numbers were determined by scoring based on allele size relative to an M13 DNA sequence ladder and to samples established as size standards that were run on each gel. NP indicates resulting PCR product was absent or of insufficient quality to enable analysis. A blank cell indicates data were not generated at this locus for that sample.
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Range_Domain:
Range_Domain_Minimum: 133
Range_Domain_Maximum: 139
Attribute_Units_of_Measure: base pairs
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: NS
Enumerated_Domain_Value_Definition:
NS indicates resulting PCR product was present, but was not scorable due to weak signal or co-amplified products indiscernible between non-specific priming vs. duplicated locus without further investigation
Enumerated_Domain_Value_Definition_Source: Author defined
Attribute:
Attribute_Label: HOPE28_allele_2
Attribute_Definition:
Microsatellite allele two from the HOPE28 locus: genotype numbers were determined by scoring based on allele size relative to an M13 DNA sequence ladder and to samples established as size standards that were run on each gel. NP indicates resulting PCR product was absent or of insufficient quality to enable analysis. A blank cell indicates data were not generated at this locus for that sample.
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Range_Domain:
Range_Domain_Minimum: 133
Range_Domain_Maximum: 148
Attribute_Units_of_Measure: base pairs
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: NS
Enumerated_Domain_Value_Definition:
NS indicates resulting PCR product was present, but was not scorable due to weak signal or co-amplified products indiscernible between non-specific priming vs. duplicated locus without further investigation
Enumerated_Domain_Value_Definition_Source: Author defined
Attribute:
Attribute_Label: HOPE35_allele_1
Attribute_Definition:
Microsatellite allele one from the HOPE35 locus: genotype numbers were determined by scoring based on allele size relative to an M13 DNA sequence ladder and to samples established as size standards that were run on each gel. NP indicates resulting PCR product was absent or of insufficient quality to enable analysis. A blank cell indicates data were not generated at this locus for that sample.
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Range_Domain:
Range_Domain_Minimum: 132
Range_Domain_Maximum: 148
Attribute_Units_of_Measure: base pairs
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: NP
Enumerated_Domain_Value_Definition:
NP indicates resulting PCR product was absent or of insufficient quality to enable analysis.
Enumerated_Domain_Value_Definition_Source: Author defined
Attribute:
Attribute_Label: HOPE35_allele_2
Attribute_Definition:
Microsatellite allele two from the HOPE35 locus: genotype numbers were determined by scoring based on allele size relative to an M13 DNA sequence ladder and to samples established as size standards that were run on each gel. NP indicates resulting PCR product was absent or of insufficient quality to enable analysis. A blank cell indicates data were not generated at this locus for that sample.
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Range_Domain:
Range_Domain_Minimum: 133
Range_Domain_Maximum: 148
Attribute_Units_of_Measure: base pairs
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: NP
Enumerated_Domain_Value_Definition:
NP indicates resulting PCR product was absent or of insufficient quality to enable analysis.
Enumerated_Domain_Value_Definition_Source: Author defined
Attribute:
Attribute_Label: HOPE38_allele_1
Attribute_Definition:
Microsatellite allele one from the HOPE38 locus: genotype numbers were determined by scoring based on allele size relative to an M13 DNA sequence ladder and to samples established as size standards that were run on each gel. NP indicates resulting PCR product was absent or of insufficient quality to enable analysis. A blank cell indicates data were not generated at this locus for that sample.
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Range_Domain:
Range_Domain_Minimum: 145
Range_Domain_Maximum: 148
Attribute_Units_of_Measure: base pairs
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: NP
Enumerated_Domain_Value_Definition:
NP indicates resulting PCR product was absent or of insufficient quality to enable analysis.
Enumerated_Domain_Value_Definition_Source: Author defined
Attribute:
Attribute_Label: HOPE38_allele_2
Attribute_Definition:
Microsatellite allele two from the HOPE38 locus: genotype numbers were determined by scoring based on allele size relative to an M13 DNA sequence ladder and to samples established as size standards that were run on each gel. NP indicates resulting PCR product was absent or of insufficient quality to enable analysis. A blank cell indicates data were not generated at this locus for that sample.
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Range_Domain:
Range_Domain_Minimum: 145
Range_Domain_Maximum: 148
Attribute_Units_of_Measure: base pairs
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: NP
Enumerated_Domain_Value_Definition:
NP indicates resulting PCR product was absent or of insufficient quality to enable analysis.
Enumerated_Domain_Value_Definition_Source: Author defined
Attribute:
Attribute_Label: HOPE40_allele_1
Attribute_Definition:
Microsatellite allele one from the HOPE40 locus: genotype numbers were determined by scoring based on allele size relative to an M13 DNA sequence ladder and to samples established as size standards that were run on each gel. NP indicates resulting PCR product was absent or of insufficient quality to enable analysis. A blank cell indicates data were not generated at this locus for that sample.
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Range_Domain:
Range_Domain_Minimum: 202
Range_Domain_Maximum: 215
Attribute_Units_of_Measure: base pairs
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: NS
Enumerated_Domain_Value_Definition:
NS indicates resulting PCR product was present, but was not scorable due to weak signal or co-amplified products indiscernible between non-specific priming vs. duplicated locus without further investigation
Enumerated_Domain_Value_Definition_Source: Author defined
Attribute:
Attribute_Label: HOPE40_allele_2
Attribute_Definition:
Microsatellite allele two from the HOPE40 locus: genotype numbers were determined by scoring based on allele size relative to an M13 DNA sequence ladder and to samples established as size standards that were run on each gel. NP indicates resulting PCR product was absent or of insufficient quality to enable analysis. A blank cell indicates data were not generated at this locus for that sample.
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Range_Domain:
Range_Domain_Minimum: 202
Range_Domain_Maximum: 215
Attribute_Units_of_Measure: base pairs
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: NS
Enumerated_Domain_Value_Definition:
NS indicates resulting PCR product was present, but was not scorable due to weak signal or co-amplified products indiscernible between non-specific priming vs. duplicated locus without further investigation
Enumerated_Domain_Value_Definition_Source: Author defined
Attribute:
Attribute_Label: HOPE42_allele_1
Attribute_Definition:
Microsatellite allele one from the HOPE42 locus: genotype numbers were determined by scoring based on allele size relative to an M13 DNA sequence ladder and to samples established as size standards that were run on each gel. NP indicates resulting PCR product was absent or of insufficient quality to enable analysis. A blank cell indicates data were not generated at this locus for that sample.
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Range_Domain:
Range_Domain_Minimum: 99
Range_Domain_Maximum: 103
Attribute_Units_of_Measure: base pairs
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: NP
Enumerated_Domain_Value_Definition:
NP indicates resulting PCR product was absent or of insufficient quality to enable analysis.
Enumerated_Domain_Value_Definition_Source: Author defined
Attribute:
Attribute_Label: HOPE42_allele_2
Attribute_Definition:
Microsatellite allele two from the HOPE42 locus: genotype numbers were determined by scoring based on allele size relative to an M13 DNA sequence ladder and to samples established as size standards that were run on each gel. NP indicates resulting PCR product was absent or of insufficient quality to enable analysis. A blank cell indicates data were not generated at this locus for that sample.
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Range_Domain:
Range_Domain_Minimum: 102
Range_Domain_Maximum: 108
Attribute_Units_of_Measure: base pairs
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: NP
Enumerated_Domain_Value_Definition:
NP indicates resulting PCR product was absent or of insufficient quality to enable analysis.
Enumerated_Domain_Value_Definition_Source: Author defined
Attribute:
Attribute_Label: HOPE43_allele_1
Attribute_Definition:
Microsatellite allele one from the HOPE43 locus: genotype numbers were determined by scoring based on allele size relative to an M13 DNA sequence ladder and to samples established as size standards that were run on each gel. NP indicates resulting PCR product was absent or of insufficient quality to enable analysis. A blank cell indicates data were not generated at this locus for that sample.
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Range_Domain:
Range_Domain_Minimum: 112
Range_Domain_Maximum: 121
Attribute_Units_of_Measure: base pairs
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: NS
Enumerated_Domain_Value_Definition:
NS indicates resulting PCR product was present, but was not scorable due to weak signal or co-amplified products indiscernible between non-specific priming vs. duplicated locus without further investigation
Enumerated_Domain_Value_Definition_Source: Author defined
Attribute:
Attribute_Label: HOPE43_allele_2
Attribute_Definition:
Microsatellite allele two from the HOPE43 locus: genotype numbers were determined by scoring based on allele size relative to an M13 DNA sequence ladder and to samples established as size standards that were run on each gel. NP indicates resulting PCR product was absent or of insufficient quality to enable analysis. A blank cell indicates data were not generated at this locus for that sample.
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Range_Domain:
Range_Domain_Minimum: 112
Range_Domain_Maximum: 121
Attribute_Units_of_Measure: base pairs
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: NS
Enumerated_Domain_Value_Definition:
NS indicates resulting PCR product was present, but was not scorable due to weak signal or co-amplified products indiscernible between non-specific priming vs. duplicated locus without further investigation
Enumerated_Domain_Value_Definition_Source: Author defined
Attribute:
Attribute_Label: HOPE43_allele_3
Attribute_Definition:
Microsatellite allele three from the HOPE43 locus: genotype numbers were determined by scoring based on allele size relative to an M13 DNA sequence ladder and to samples established as size standards that were run on each gel. NOTE: locus HOPE43_allele_3 is presumed to be the result of a polyploid individual. A blank cell indicates a third allele was not observed at this locus for that sample.
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Range_Domain:
Range_Domain_Minimum: 121
Range_Domain_Maximum: 121
Attribute_Units_of_Measure: base pairs
Attribute:
Attribute_Label: HOPE49_allele_1
Attribute_Definition:
Microsatellite allele one from the HOPE49 locus: genotype numbers were determined by scoring based on allele size relative to an M13 DNA sequence ladder and to samples established as size standards that were run on each gel. NP indicates resulting PCR product was absent or of insufficient quality to enable analysis. A blank cell indicates data were not generated at this locus for that sample.
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Range_Domain:
Range_Domain_Minimum: 155
Range_Domain_Maximum: 164
Attribute_Units_of_Measure: base pairs
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: NS
Enumerated_Domain_Value_Definition:
NS indicates resulting PCR product was present, but was not scorable due to weak signal or co-amplified products indiscernible between non-specific priming vs. duplicated locus without further investigation
Enumerated_Domain_Value_Definition_Source: Author defined
Attribute:
Attribute_Label: HOPE49_allele_2
Attribute_Definition:
Microsatellite allele two from the HOPE49 locus: genotype numbers were determined by scoring based on allele size relative to an M13 DNA sequence ladder and to samples established as size standards that were run on each gel. NP indicates resulting PCR product was absent or of insufficient quality to enable analysis. A blank cell indicates data were not generated at this locus for that sample.
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Range_Domain:
Range_Domain_Minimum: 155
Range_Domain_Maximum: 173
Attribute_Units_of_Measure: base pairs
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: NS
Enumerated_Domain_Value_Definition:
NS indicates resulting PCR product was present, but was not scorable due to weak signal or co-amplified products indiscernible between non-specific priming vs. duplicated locus without further investigation
Enumerated_Domain_Value_Definition_Source: Author defined
Attribute:
Attribute_Label: HOPE58_allele_1
Attribute_Definition:
Microsatellite allele one from the HOPE58 locus: genotype numbers were determined by scoring based on allele size relative to an M13 DNA sequence ladder and to samples established as size standards that were run on each gel. NP indicates resulting PCR product was absent or of insufficient quality to enable analysis. A blank cell indicates data were not generated at this locus for that sample.
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Range_Domain:
Range_Domain_Minimum: 126
Range_Domain_Maximum: 132
Attribute_Units_of_Measure: base pairs
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: NP
Enumerated_Domain_Value_Definition:
NP indicates resulting PCR product was absent or of insufficient quality to enable analysis.
Enumerated_Domain_Value_Definition_Source: Author defined
Attribute:
Attribute_Label: HOPE58_allele_2
Attribute_Definition:
Microsatellite allele two from the HOPE58 locus: genotype numbers were determined by scoring based on allele size relative to an M13 DNA sequence ladder and to samples established as size standards that were run on each gel. NP indicates resulting PCR product was absent or of insufficient quality to enable analysis. A blank cell indicates data were not generated at this locus for that sample.
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Range_Domain:
Range_Domain_Minimum: 126
Range_Domain_Maximum: 132
Attribute_Units_of_Measure: base pairs
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: NP
Enumerated_Domain_Value_Definition:
NP indicates resulting PCR product was absent or of insufficient quality to enable analysis.
Enumerated_Domain_Value_Definition_Source: Author defined
Distribution_Information:
Distributor:
Contact_Information:
Contact_Organization_Primary:
Contact_Organization: U.S. Geological Survey, Alaska Science Center
Contact_Address:
Address_Type: mailing and physical
Address: 4210 University Dr.
City: Anchorage
State_or_Province: Alaska
Postal_Code: 99508
Country: USA
Contact_Voice_Telephone: 907-786-7000
Contact_Electronic_Mail_Address: ascweb@usgs.gov
Resource_Description: Authoritative source for access to data.
Distribution_Liability:
Unless otherwise stated, all data, metadata and related materials are considered to satisfy the quality standards relative to the purpose for which the data were collected. Although these data and associated metadata have been reviewed for accuracy and completeness and approved for release by the U.S. Geological Survey (USGS), no warranty expressed or implied is made regarding the display or utility of the data on any other system or for general or scientific purposes, nor shall the act of distribution constitute any such warranty
Standard_Order_Process:
Digital_Form:
Digital_Transfer_Information:
Format_Name: Zip
Format_Version_Number: 19.0
Format_Information_Content:
Zip file containing the data in CSV file format and FGDC metadata in XML and HTML formats.
File_Decompression_Technique: Winzip legacy compression applied
Digital_Transfer_Option:
Online_Option:
Computer_Contact_Information:
Network_Address:
Network_Resource_Name: https://doi.org/10.5066/F7BC3XRP
Fees: No fees.
Metadata_Reference_Information:
Metadata_Date: 20201125
Metadata_Review_Date: 20171205
Metadata_Contact:
Contact_Information:
Contact_Organization_Primary:
Contact_Organization: U.S. Geological Survey, Alaska Science Center
Contact_Address:
Address_Type: mailing and physical
Address: 4210 University Drive
City: Anchorage
State_or_Province: Alaska
Postal_Code: 99508
Country: USA
Contact_Voice_Telephone: 907-786-7000
Contact_Electronic_Mail_Address: ascweb@usgs.gov
Metadata_Standard_Name:
FGDC Biological Data Profile of the Content Standard for Digital Geospatial Metadata
Metadata_Standard_Version: FGDC-STD-001.1-1999

Generated by mp version 2.9.47 on Fri May 18 16:41:57 2018