Entity_Type:
Entity_Type_Label:
Enteroctopus Sampling Effects on Genetic Data, Prince William Sound Alaska, 2012-2015
Entity_Type_Definition: Data set
Entity_Type_Definition_Source: Author defined
Attribute:
Attribute_Label: Sample ID
Attribute_Definition:
This is the original label assigned in the field to each individual sample received in the laboratory.
Attribute_Definition_Source: Field assigned
Attribute_Domain_Values:
Unrepresentable_Domain:
These are field assigned sample labels as received by the laboratory. The extension with "replicate 1, 2, 3 or 4" has been added to differentiate treatment groups for this laboratory method validation study.
Attribute:
Attribute_Label: Lab ID
Attribute_Definition:
Unique sample ID including genus, individual, sample type, replicate (if appropriate), and designation of type of delayed extraction (if appropriate).
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Unrepresentable_Domain:
This is a 10-20 character (including spaces) label generated for laboratory identification of samples which includes a 4 letter taxonomic code (ENDO), 3-8 alphabetical or alphanumerical code corresponding to original field labelled Sample ID and 1-6 character code indicating sample type ("T" or "S" for tissue or swab, respectively), whether the swab was the first or second in the sample ("1" or "2"), the time to extraction after receipt in the lab (blank for immediate and "A" or "C" for 8-31 months post-receipt; "A" if via salt or "C" if via CTAB protocols, and if the sample was extracted in duplicate ["(1)"] for quality control of the extraction as assessed by DNA yield.
Attribute:
Attribute_Label: Collection Date
Attribute_Definition:
This is the season (either Autumn or Spring) and year (2012 or 2013) the sample was collected from the field.
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: Autumn 2012
Enumerated_Domain_Value_Definition: This denotes an octopus captured in autumn 2012.
Enumerated_Domain_Value_Definition_Source: Author defined
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: Spring 2013
Enumerated_Domain_Value_Definition: This denotes an octopus captured in spring 2013.
Enumerated_Domain_Value_Definition_Source: Author defined
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: Autumn 2013
Enumerated_Domain_Value_Definition: This denotes an octopus captured in autumn 2013.
Enumerated_Domain_Value_Definition_Source: Author defined
Attribute:
Attribute_Label: Lineage
Attribute_Definition:
Lineage assignment to either Giant Pacific Octopus (GPO) or Cryptic based on morphology in the field and confirmed by rapid screen of 2 diagnostic microsatellite markers in this data set.
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: GPO
Enumerated_Domain_Value_Definition:
Species designation of Giant Pacific Octopus (Enteroctopus dofleini; GPO) as determined by morphology in the field and confirmed by laboratory DNA analyses in this data set.
Enumerated_Domain_Value_Definition_Source: Author defined
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: Cryptic
Enumerated_Domain_Value_Definition:
Novel morphotypes from field measurements and DNA haplotypes from this data set preclude assignment of these "cryptic lineage" samples to currently recognized Giant Pacific Octopus (Enteroctopus dofleini).
Enumerated_Domain_Value_Definition_Source: Author defined
Attribute:
Attribute_Label: Field Sex
Attribute_Definition:
This is the sex of the individual as determined by morphology in the field and provided on data sheets with the samples upon arrival in the laboratory.
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: F
Enumerated_Domain_Value_Definition:
Individual identified as a female based on morphology in the field.
Enumerated_Domain_Value_Definition_Source: Author defined
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: M
Enumerated_Domain_Value_Definition:
Individual identified as a male based on morphology in the field.
Enumerated_Domain_Value_Definition_Source: Author defined
Attribute:
Attribute_Label: Depth (m)
Attribute_Definition:
This is the ocean depth at or range of depth in which a sample was collected according to information provided to the laboratory by biologists.
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Range_Domain:
Range_Domain_Minimum: 54.9
Range_Domain_Maximum: 185
Attribute_Units_of_Measure: Meters
Attribute:
Attribute_Label: Sampling Location
Attribute_Definition:
This is the specific geographic area in which the sample was collected.
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: Bald Head Chris
Enumerated_Domain_Value_Definition: Bald Head Chris Island, Alaska
Enumerated_Domain_Value_Definition_Source: Geographic Names Information System
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: Golden Bay, Port Wells
Enumerated_Domain_Value_Definition:
Golden Bay in Port Wells, Alaska (presumed to be the bay at the mouth of the Golden River (60.96697 N, 147.99572 W; 60.96606 N, 147.98176 W) coming from Davis Lake north of Esther Island)
Enumerated_Domain_Value_Definition_Source: Author defined
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: Long Bay
Enumerated_Domain_Value_Definition: Long Bay, Alaska
Enumerated_Domain_Value_Definition_Source: Geographic Names Information System
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: Western Prince William Sound
Enumerated_Domain_Value_Definition:
A general area presumed to be some smaller "western" area than the overall geographic bounding of "Prince William Sound" as a whole.
Enumerated_Domain_Value_Definition_Source: Field assigned
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: Prince of Wales Passage
Enumerated_Domain_Value_Definition: Prince of Wales Passage, Alaska
Enumerated_Domain_Value_Definition_Source: Geographic Names Information System
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: Chenega
Enumerated_Domain_Value_Definition:
Chenega, Alaska (presumed to be from the waters surrounding Chenega Island, Prince William Sound Alaska (60.3147222 N, 148.0716667 W; 60.3102778 N, 148.1305556 W)
Enumerated_Domain_Value_Definition_Source: Author defined
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: Herring Bay
Enumerated_Domain_Value_Definition: Herring Bay, Alaska
Enumerated_Domain_Value_Definition_Source: Geographic Names Information System
Attribute:
Attribute_Label: Locale
Attribute_Definition:
This is the general geographic area in which the sample was collected.
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: Prince William Sound
Enumerated_Domain_Value_Definition: Prince William Sound, Alaska
Enumerated_Domain_Value_Definition_Source: Geographic Names Information System
Attribute:
Attribute_Label: Extraction
Attribute_Definition:
This indicates the method by which DNA was extracted from the sample.
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: CTAB
Enumerated_Domain_Value_Definition:
This refers to a published DNA extraction method with modifications by the author to avoid analysis complications caused by mucopolysaccarides in DNA samples (Hollenbeck, N. et al. 2017. Use of swabs for sampling epithelial cells for molecular genetics analyses in Enteroctopus. Am. Malacol. Bull.).
Enumerated_Domain_Value_Definition_Source: Author defined
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: MVZ salt
Enumerated_Domain_Value_Definition:
This refers to a published DNA extraction method with modifications by the author which reduces cost and time required compared to many commercial DNA extraction kits (Sonsthagen, S.A., et al. 2004. Gene flow and genetic characterization of Northern Goshawks breeding in Utah. Condor 106: 826–836.).
Enumerated_Domain_Value_Definition_Source: Author defined
Attribute:
Attribute_Label: DNA Yield
Attribute_Definition:
This is the concentration of extracted DNA of each sample as determined with fluorometry in relation to a known standard (100 nanograms/microliter) of calf thymus DNA. All DNA's used in subsequent PCR amplifications were diluted to working stocks of 50 nanograms/microliter.
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Range_Domain:
Range_Domain_Minimum: 2
Range_Domain_Maximum: 765
Attribute_Units_of_Measure: nanograms of DNA per microliter (ng/ul)
Attribute:
Attribute_Label: GenBank OCDE
Attribute_Definition:
This is the GenBank (
https://www.ncbi.nlm.nih.gov/genbank/) accession number for associated nuclear octopine dehydrogenase gene nucleotide sequence data associated with a particular sample, where applicable. Blank cells indicate replicate samples for which sequences were not generated or samples for which sequence matching previously identified alleles were collected (see attributes "ALLELE1" and "ALLELE2" to differentiate between no sequence generated and non-novel sequence generated).
Range_Domain:
Range_Domain_Minimum: KT225573
Range_Domain_Maximum: KT225574
Attribute_Units_of_Measure: GenBank accession number
Attribute:
Attribute_Label: Manually Edited BP
Attribute_Definition:
Average number of base pairs of forward and reverse nuclear octopine dehydrogenase(OCDE) sequence manually edited after auto-sequencing of the sample using Licor's eSeq v. 3.1 and AlignIR 2.0. An asterisk (*) indicates the sequence required greater than 20% manual editing, is considered an outlier for the sequencing reaction results and was not used in manuscript pairwise comparisons of tissue versus swab results.
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Range_Domain:
Range_Domain_Minimum: 21
Range_Domain_Maximum: 140.5
Attribute_Units_of_Measure: base pairs
Attribute:
Attribute_Label: Auto-called BP
Attribute_Definition:
Average number of base pairs of forward and reverse nuclear octopine dehydrogenase(OCDE) sequence auto-sequenced and accepted as called using Licor's eSeq v. 3.1 and AlignIR 2.0(trademark), LI-COR Inc., Lincoln, NE.
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Range_Domain:
Range_Domain_Minimum: 360.5
Range_Domain_Maximum: 550
Attribute_Units_of_Measure: base pairs
Attribute:
Attribute_Label: Quality (SD)
Attribute_Definition:
These are the phred quality (Q) scores and base calling error rate (in standard deviation or SD) provided by the sequencing alignment package AlignIR 2.0 (trademark), LI-COR Inc., Lincoln, NE).
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Range_Domain:
Range_Domain_Minimum: 25.72 (9.78)
Range_Domain_Maximum: 37.38 (7.38)
Attribute_Units_of_Measure: phred quality (Q) scores (standard deviation)
Attribute:
Attribute_Label: OCDE_1
Attribute_Definition:
This is the first of two allele assignments for the nuclear octopine dehydrogenase (OCDE) gene based on up to 6 site substitutions from 528 base pairs of nucleotide sequence data. A blank cell indicates data were not generated at this locus for that sample. As a diploid locus, data from OCDE_1 must only be considered in relation to data for OCDE_2 for the same individual.
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Codeset_Domain:
Codeset_Name: OCDE Alleles
Codeset_Source:
Assignment of OCDE1, OCDE2, or OCDE3 corresponds with sequence data matching alleles described in Genbank under accession numbers KT213460 (OCDE1), KT213461 (OCDE2), and KT225573 (OCDE3).
Attribute:
Attribute_Label: OCDE_2
Attribute_Definition:
This is the second of two allele assignments for the nuclear octopine dehydrogenase (OCDE) gene based on up to 6 site substitutions from 528 base pairs of nucleotide sequence data. A blank cell indicates data were not generated at this locus for that sample. As a diploid locus, data from OCDE_2 must only be considered in relation to data for OCDE_1 for the same individual.
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Codeset_Domain:
Codeset_Name: OCDE Alleles
Codeset_Source:
Assignment of OCDE1, OCDE2, OCDE3, or OCDE4 corresponds with sequence data matching alleles described in Genbank under accession numbers KT213460 (OCDE1), KT213461 (OCDE2), KT225573 (OCDE3), and KT225574 (OCDE4);
Attribute:
Attribute_Label: EdoµA6_1
Attribute_Definition:
Microsatellite allele one of the EdoµA6 locus: genotype numbers were determined by scoring based on allele size relative to an M13 DNA sequence ladder and to samples established as size standards that were run on each gel. NP indicates resulting PCR product was absent or of insufficient quality to enable analysis. A blank cell indicates data were not generated at this locus for that sample. As a diploid locus, data from allele one must only be considered in relation to data for allele two for the same individual.
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Range_Domain:
Range_Domain_Minimum: 168
Range_Domain_Maximum: 202
Attribute_Units_of_Measure: base pairs
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: NP
Enumerated_Domain_Value_Definition:
Designation as "NP" indicates no product was visible precluding size determination at this locus.
Enumerated_Domain_Value_Definition_Source: Author defined
Attribute:
Attribute_Label: EdoµA6_2
Attribute_Definition:
Microsatellite allele two from the EdoµA6 locus: genotype numbers were determined by scoring based on allele size relative to an M13 DNA sequence ladder and to samples established as size standards that were run on each gel. NP indicates resulting PCR product was absent or of insufficient quality to enable analysis. A blank cell indicates data were not generated at this locus for that sample. As a diploid locus, data from allele two must only be considered in relation to data for allele one for the same individual.
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Range_Domain:
Range_Domain_Minimum: 190
Range_Domain_Maximum: 228
Attribute_Units_of_Measure: base pairs
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: NP
Enumerated_Domain_Value_Definition:
Designation as "NP" indicates no product was visible precluding size determination at this locus.
Enumerated_Domain_Value_Definition_Source: Author defined
Attribute:
Attribute_Label: EdoµA8_1
Attribute_Definition:
Microsatellite allele one of the EdoµA8 locus: genotype numbers were determined by scoring based on allele size relative to an M13 DNA sequence ladder and to samples established as size standards that were run on each gel. A blank cell indicates data were not generated at this locus for that sample. As a diploid locus, data from allele one must only be considered in relation to data for allele two for the same individual.
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Range_Domain:
Range_Domain_Minimum: 181
Range_Domain_Maximum: 209
Attribute_Units_of_Measure: base pairs
Attribute:
Attribute_Label: EdoµA8_2
Attribute_Definition:
Microsatellite allele two from the EdoµA8 locus: genotype numbers were determined by scoring based on allele size relative to an M13 DNA sequence ladder and to samples established as size standards that were run on each gel. A blank cell indicates data were not generated at this locus for that sample. As a diploid locus, data from allele two must only be considered in relation to data for allele one for the same individual.
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Range_Domain:
Range_Domain_Minimum: 181
Range_Domain_Maximum: 237
Attribute_Units_of_Measure: base pairs
Attribute:
Attribute_Label: EdoµA105_1
Attribute_Definition:
Microsatellite allele one of the EdoµA105 locus: genotype numbers were determined by scoring based on allele size relative to an M13 DNA sequence ladder and to samples established as size standards that were run on each gel. A blank cell indicates data were not generated at this locus for that sample. As a diploid locus, data from allele one must only be considered in relation to data for allele two for the same individual.
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Range_Domain:
Range_Domain_Minimum: 187
Range_Domain_Maximum: 203
Attribute_Units_of_Measure: base pairs
Attribute:
Attribute_Label: EdoµA105_2
Attribute_Definition:
Microsatellite allele two from the EdoµA105 locus: genotype numbers were determined by scoring based on allele size relative to an M13 DNA sequence ladder and to samples established as size standards that were run on each gel. A blank cell indicates data were not generated at this locus for that sample. As a diploid locus, data from allele two must only be considered in relation to data for allele one for the same individual.
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Range_Domain:
Range_Domain_Minimum: 191
Range_Domain_Maximum: 217
Attribute_Units_of_Measure: base pairs
Attribute:
Attribute_Label: EdoµA106_1
Attribute_Definition:
Microsatellite allele one of the EdoµA106 locus: genotype numbers were determined by scoring based on allele size relative to an M13 DNA sequence ladder and to samples established as size standards that were run on each gel. A blank cell indicates data were not generated at this locus for that sample. As a diploid locus, data from allele one must only be considered in relation to data for allele two for the same individual.
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Range_Domain:
Range_Domain_Minimum: 110
Range_Domain_Maximum: 126
Attribute_Units_of_Measure: base pairs
Attribute:
Attribute_Label: EdoµA106_2
Attribute_Definition:
Microsatellite allele two from the EdoµA106 locus: genotype numbers were determined by scoring based on allele size relative to an M13 DNA sequence ladder and to samples established as size standards that were run on each gel. A blank cell indicates data were not generated at this locus for that sample. As a diploid locus, data from allele two must only be considered in relation to data for allele one for the same individual.
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Range_Domain:
Range_Domain_Minimum: 110
Range_Domain_Maximum: 126
Attribute_Units_of_Measure: base pairs
Attribute:
Attribute_Label: EdoµA110_1
Attribute_Definition:
Microsatellite allele one of the EdoµA110 locus: genotype numbers were determined by scoring based on allele size relative to an M13 DNA sequence ladder and to samples established as size standards that were run on each gel. A blank cell indicates data were not generated at this locus for that sample. As a diploid locus, data from allele one must only be considered in relation to data for allele two for the same individual.
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Range_Domain:
Range_Domain_Minimum: 124
Range_Domain_Maximum: 164
Attribute_Units_of_Measure: base pairs
Attribute:
Attribute_Label: EdoµA110_2
Attribute_Definition:
Microsatellite allele two from the EdoµA110 locus: genotype numbers were determined by scoring based on allele size relative to an M13 DNA sequence ladder and to samples established as size standards that were run on each gel. A blank cell indicates data were not generated at this locus for that sample. As a diploid locus, data from allele two must only be considered in relation to data for allele one for the same individual.
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Range_Domain:
Range_Domain_Minimum: 126
Range_Domain_Maximum: 164
Attribute_Units_of_Measure: base pairs
Attribute:
Attribute_Label: EdoµA118_1
Attribute_Definition:
Microsatellite allele one of the EdoµA118 locus: genotype numbers were determined by scoring based on allele size relative to an M13 DNA sequence ladder and to samples established as size standards that were run on each gel. A blank cell indicates data were not generated at this locus for that sample. As a diploid locus, data from allele one must only be considered in relation to data for allele two for the same individual.
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Range_Domain:
Range_Domain_Minimum: 225
Range_Domain_Maximum: 253
Attribute_Units_of_Measure: base pairs
Attribute:
Attribute_Label: EdoµA118_2
Attribute_Definition:
Microsatellite allele two from the EdoµA118 locus: genotype numbers were determined by scoring based on allele size relative to an M13 DNA sequence ladder and to samples established as size standards that were run on each gel. A blank cell indicates data were not generated at this locus for that sample. As a diploid locus, data from allele two must only be considered in relation to data for allele one for the same individual.
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Range_Domain:
Range_Domain_Minimum: 227
Range_Domain_Maximum: 303
Attribute_Units_of_Measure: base pairs
Attribute:
Attribute_Label: EdoµA122_1
Attribute_Definition:
Microsatellite allele one of the EdoµA122 locus: genotype numbers were determined by scoring based on allele size relative to an M13 DNA sequence ladder and to samples established as size standards that were run on each gel. A blank cell indicates data were not generated at this locus for that sample. As a diploid locus, data from allele one must only be considered in relation to data for allele two for the same individual.
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Range_Domain:
Range_Domain_Minimum: 178
Range_Domain_Maximum: 219
Attribute_Units_of_Measure: base pairs
Attribute:
Attribute_Label: EdoµA122_2
Attribute_Definition:
Microsatellite allele two from the EdoµA122 locus: genotype numbers were determined by scoring based on allele size relative to an M13 DNA sequence ladder and to samples established as size standards that were run on each gel. A blank cell indicates data were not generated at this locus for that sample. As a diploid locus, data from allele two must only be considered in relation to data for allele one for the same individual.
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Range_Domain:
Range_Domain_Minimum: 182
Range_Domain_Maximum: 241
Attribute_Units_of_Measure: base pairs
Attribute:
Attribute_Label: EdoµA125_1
Attribute_Definition:
Microsatellite allele one of the EdoµA125 locus: genotype numbers were determined by scoring based on allele size relative to an M13 DNA sequence ladder and to samples established as size standards that were run on each gel. A blank cell indicates data were not generated at this locus for that sample. As a diploid locus, data from allele one must only be considered in relation to data for allele two for the same individual.
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Range_Domain:
Range_Domain_Minimum: 162
Range_Domain_Maximum: 222
Attribute_Units_of_Measure: base pairs
Attribute:
Attribute_Label: EdoµA125_2
Attribute_Definition:
Microsatellite allele two from the EdoµA125 locus: genotype numbers were determined by scoring based on allele size relative to an M13 DNA sequence ladder and to samples established as size standards that were run on each gel. A blank cell indicates data were not generated at this locus for that sample. As a diploid locus, data from allele two must only be considered in relation to data for allele one for the same individual.
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Range_Domain:
Range_Domain_Minimum: 172
Range_Domain_Maximum: 268
Attribute_Units_of_Measure: base pairs
Attribute:
Attribute_Label: EdoµA129_1
Attribute_Definition:
Microsatellite allele one of the EdoµA129 locus: genotype numbers were determined by scoring based on allele size relative to an M13 DNA sequence ladder and to samples established as size standards that were run on each gel. A blank cell indicates data were not generated at this locus for that sample. As a diploid locus, data from allele one must only be considered in relation to data for allele two for the same individual.
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Range_Domain:
Range_Domain_Minimum: 127
Range_Domain_Maximum: 153
Attribute_Units_of_Measure: base pairs
Attribute:
Attribute_Label: EdoµA129_2
Attribute_Definition:
Microsatellite allele two from the EdoµA129 locus: genotype numbers were determined by scoring based on allele size relative to an M13 DNA sequence ladder and to samples established as size standards that were run on each gel. A blank cell indicates data were not generated at this locus for that sample. As a diploid locus, data from allele two must only be considered in relation to data for allele one for the same individual.
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Range_Domain:
Range_Domain_Minimum: 127
Range_Domain_Maximum: 155
Attribute_Units_of_Measure: base pairs
Attribute:
Attribute_Label: EdoµE11_1
Attribute_Definition:
Microsatellite allele one of the EdoµE11 locus: genotype numbers were determined by scoring based on allele size relative to an M13 DNA sequence ladder and to samples established as size standards that were run on each gel. A blank cell indicates data were not generated at this locus for that sample. As a diploid locus, data from allele one must only be considered in relation to data for allele two for the same individual.
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Range_Domain:
Range_Domain_Minimum: 135
Range_Domain_Maximum: 211
Attribute_Units_of_Measure: base pairs
Attribute:
Attribute_Label: EdoµE11_2
Attribute_Definition:
Microsatellite allele two from the EdoµE11 locus: genotype numbers were determined by scoring based on allele size relative to an M13 DNA sequence ladder and to samples established as size standards that were run on each gel. A blank cell indicates data were not generated at this locus for that sample. As a diploid locus, data from allele two must only be considered in relation to data for allele one for the same individual.
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Range_Domain:
Range_Domain_Minimum: 139
Range_Domain_Maximum: 219
Attribute_Units_of_Measure: base pairs