Specimen and Genetic Information for Phylogeny of Blue-winged Ducks (Anas spp.), 2001-2011

We used previously published genetic datasets of South American cinnamon teal (mtDNA and 5 nuclear introns) and North American cinnamon and blue-winged teal (mtDNA and 2 nuclear intron). From these studies, vouchered specimens of A. c. cyanoptera (N = 52 individuals), A. c. orinomus (N = 49) A. c. septentrionalium (N = 70), and A. discors (N = 76) were collected in Argentina, Bolivia, Colombia, Peru, and the United States. Homologous data for northern shovelers from widespread locations in North America (N = 48) and Eurasia (N = 36) were obtained. A. smithii samples were collected from southern Africa (N = 24), vouchered specimens of A. platalea were collected from central and southern South America (N = 24), and A. rhynchotis were collected from Australia (N = 19) and New Zealand (N = 4). See Nelson et al. (2016) for details.

Genomic DNA was extracted from muscle tissue or blood using a Qiagen DNeasy tissue kit. For each sample, we amplified and sequenced five nuclear introns (ENO1, PCK1, ODC1, GRIN1, and FGB; collective total of 1,501bp) and the mitochondrial control region (658–983bp), following protocols described in Nelson et al. (2016). PCR products were cleaned using magnetic AMPure beads (Beckman Coulter, INC., Indianapolis, IN). Direct sequencing was performed on an ABI 3730. All sequences for A. c. cyanoptera, A. c. orinomus, and A. clypeata, as well as mtDNA, ENO1 and ODC1 sequences for A. c. septentrionalium and A. discors, have been published previously. See Nelson et al. (2016) for details.