Blood Parasite Infection Data from Emperor Geese (Anser canagicus) and Cackling Geese (Branta hutchinsii minima), Yukon-Kuskokwim Delta, Alaska, 1998-2014

Metadata:

Identification_Information:
Citation:
Citation_Information:
Originator: Reed, J.A. (ORCID: 0000-0002-3239-6909)
Originator: Buchheit, R.M.
Originator: Schmutz, J.A. (ORCID: 0000-0002-6516-0836)
Originator: Uher-Koch, B.D. (ORCID: 0000-0002-1885-0260)
Originator: Ramey, A.M. (ORCID: 0000-0002-3601-8400)
Publication_Date: 20210310
Title:
Blood Parasite Infection Data from Emperor Geese (Anser canagicus) and Cackling Geese (Branta hutchinsii minima), Yukon-Kuskokwim Delta, Alaska, 1998-2014
Geospatial_Data_Presentation_Form: tabular digital data
Publication_Information:
Publication_Place: Anchorage, Alaska
Publisher: U.S. Geological Survey, Alaska Science Center
Other_Citation_Details:
Suggested Citation: Reed, J.A., Buchheit, R.M., Schmutz, J.A., Uher-Koch, B.D., Ramey, A.M., 2021, Blood parasite infection data from Emperor geese (Anser canagicus) and Cackling geese (Branta hutchinsii minima), Yukon-Kuskokwim Delta, Alaska, 1998-2014: U.S. Geological Survey data release, https://doi.org/10.5066/P9F7LD2I
Online_Linkage: https://doi.org/10.5066/P9F7LD2I
Larger_Work_Citation:
Citation_Information:
Originator: U.S. Geological Survey, Alaska Science Center
Publication_Date: 2011
Title:
Assessment of wildlife health and disease in Alaska and adjacent regions
Geospatial_Data_Presentation_Form: website
Series_Information:
Series_Name: Alaska Science Portal
Issue_Identification: 321
Publication_Information:
Publication_Place: Anchorage, Alaska
Publisher: U.S. Geological Survey, Alaska Science Center
Other_Citation_Details:
This is a link to the broader USGS Alaska Science Center research project supported by these data. Users will find a description of the research project and links to associated reports, publications, and data products.
Online_Linkage: https://alaska.usgs.gov/portal/project.php?project_id=321
Description:
Abstract:
This dataset includes one table of blood parasite (hematozoa) infection data from 197 Emperor Geese (Anser canagicus) and 110 Cackling Geese (Branta hutchinsii minima) sampled on the Yukon-Kuskokwim Delta, Alaska, in 1998 and 2014. Three genera of blood parasites (Leucocytozoon, Haemoproteus, Plasmodium) were detected by molecular sequencing of a 479 bp fragment of the Cytochrome-b gene. The table provides age, sex, infection status, and NCBI GenBank accession numbers of the DNA sequences obtained from infected samples.
Purpose:
These data provided the basis for a study of detection, prevalence, and transmission of avian hematozoa (blood parasites) in Emperor Geese and Cackling Geese in western Alaska breeding areas.
Time_Period_of_Content:
Time_Period_Information:
Range_of_Dates/Times:
Beginning_Date: 1998
Ending_Date: 2014
Currentness_Reference: observed
Status:
Progress: Complete
Maintenance_and_Update_Frequency: None planned
Spatial_Domain:
Description_of_Geographic_Extent:
Blood samples were collected from geese nesting near the mouth of the Manokinak River in the Yukon-Kuskokwim Delta National Wildlife Refuge, Alaska, USA
Bounding_Coordinates:
West_Bounding_Coordinate: -165.2
East_Bounding_Coordinate: -165.1
North_Bounding_Coordinate: 61.2
South_Bounding_Coordinate: 61.1
Keywords:
Theme:
Theme_Keyword_Thesaurus: USGS Metadata Identifier
Theme_Keyword: USGS:ASC357
Theme:
Theme_Keyword_Thesaurus: ISO 19115 Topic Category
Theme_Keyword: Biota
Theme_Keyword: Environment
Theme:
Theme_Keyword_Thesaurus: NASA GCMD Earth Science Keyword Thesaurus
Theme_Keyword: Animals/Vertebrates
Theme_Keyword: Birds
Theme_Keyword: Ducks/Geese/Swans
Theme_Keyword: Protists
Theme_Keyword: Parasitism
Theme_Keyword: Species life history
Theme:
Theme_Keyword_Thesaurus: USGS CSA Biocomplexity Thesaurus
Theme_Keyword: Migratory birds
Theme_Keyword: Waterfowl
Theme_Keyword: Parasitic diseases
Theme_Keyword: Vector borne diseases
Theme:
Theme_Keyword_Thesaurus: USGS Thesaurus
Theme_Keyword: Wildlife
Theme_Keyword: Tundra ecosystems
Theme_Keyword: Wetland ecosystems
Theme_Keyword: Wildlife disease
Theme_Keyword: Parasitology
Theme_Keyword: Genetics
Theme_Keyword: Polymerase chain reaction
Theme_Keyword: DNA Sequencing
Theme:
Theme_Keyword_Thesaurus: NIH MeSH Thesaurus
Theme_Keyword: Parasitology
Theme:
Theme_Keyword_Thesaurus: None
Theme_Keyword: Emperor goose
Theme_Keyword: Anser canigicus
Theme_Keyword: Cackling goose
Theme_Keyword: Branta canadensis minima
Theme_Keyword: Blood Parasites
Theme_Keyword: Haematozoa
Theme_Keyword: Leucocytozoon
Theme_Keyword: Haemoproteus
Theme_Keyword: Plasmodium
Place:
Place_Keyword_Thesaurus: USGS Geographic Names Information System (GNIS)
Place_Keyword: Alaska
Place_Keyword: Yukon Delta National Wildlife Refuge
Place_Keyword: Manokinak River
Place:
Place_Keyword_Thesaurus: None
Place_Keyword: Yukon-Kuskokwim Delta
Taxonomy:
Keywords/Taxon:
Taxonomic_Keyword_Thesaurus: None
Taxonomic_Keywords: Animals
Taxonomic_Keywords: Birds
Taxonomic_Keywords: Waterfowl
Taxonomic_Keywords: Geese
Taxonomic_Keywords: Protists
Taxonomic_Keywords: Hematozoa
Taxonomic_System:
Classification_System/Authority:
Classification_System_Citation:
Citation_Information:
Originator: ITIS Integrated Taxonomic Information System
Publication_Date: Unknown
Title: ITIS Integrated Taxonomic Information System
Geospatial_Data_Presentation_Form: online database
Publication_Information:
Publication_Place: online
Publisher: ITIS-North America
Other_Citation_Details:
Taxonomic details retrieved January 25, 2021, from the Integrated Taxonomic Information System online database: https://www.itis.gov
Online_Linkage: https://www.itis.gov
Classification_System_Modifications:
ITIS currently lists Emperor Geese in the genus Chen. However, the most current taxonomic designation by the American Ornithological Society's (AOU) Check-list of North American Birds ( http://checklist.aou.org/taxa ) considers Emperor Geese in the genus Anser.
Here, we follow the current AOU designation as described in: Chesser, R.T., Burns, K.J., Cicero, C., Dunn, J.L., Kratter, A.W., Lovette, I.J., Rasmussen, P.C., Remsen, J.V., Jr., Rising, J.D., Stotz, D.F., Winker, K. 2017. Fifty-eighth supplement to the American Ornithological Society's Check-list of North American Birds. The Auk:134(3)751-773 https://doi.org/10.1642/AUK-17-72.1
Classification_System/Authority:
Classification_System_Citation:
Citation_Information:
Originator: NCBI Taxonomy database
Publication_Date: Unknown
Title: National Center for Biotechnology Information Taxonomy Database
Geospatial_Data_Presentation_Form: database
Publication_Information:
Publication_Place: online
Publisher: National Center for Biotechnology Information
Other_Citation_Details:
Taxonomy details for Hematozoa were retrieved January 25, 2021, from the National Center for Biotechnology Information (NCBI) Taxonomy database on-line
Online_Linkage: https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi
Classification_System_Modifications: None
Identification_Reference:
Citation_Information:
Originator: Valkiunas, G.
Publication_Date: 2004
Title: Avian malaria parasites and other haemosporidia
Geospatial_Data_Presentation_Form: book chapter
Publication_Information:
Publication_Place: Boca Raton, Florida (USA)
Publisher: CRC Press
Other_Citation_Details:
Valkiunas, G., 2004, Avian malaria parasites and other haemosporidia. CRC Press, Boca Raton, FL. 932pp.
Taxonomic_Procedures:
Bird species were identified by skilled observers in the field based on general appearance of feathers, plumage, behavior, and vocalization. Hematozoa were identified by comparison of DNA sequences to sequences in NCBI GenBank.
Taxonomic_Completeness:
Taxonomy is complete for all samples. No voucher specimens were collected.
Taxonomic_Classification:
Taxon_Rank_Name: Life
Taxon_Rank_Value: Life
Taxonomic_Classification:
Taxon_Rank_Name: Kingdom
Taxon_Rank_Value: Animalia
Taxonomic_Classification:
Taxon_Rank_Name: Subkingdom
Taxon_Rank_Value: Bilateria
Taxonomic_Classification:
Taxon_Rank_Name: Infrakingdom
Taxon_Rank_Value: Deuterostomia
Taxonomic_Classification:
Taxon_Rank_Name: Phylum
Taxon_Rank_Value: Chordata
Taxonomic_Classification:
Taxon_Rank_Name: Subphylum
Taxon_Rank_Value: Vertebrata
Taxonomic_Classification:
Taxon_Rank_Name: Infraphylum
Taxon_Rank_Value: Gnathostomata
Taxonomic_Classification:
Taxon_Rank_Name: Superclass
Taxon_Rank_Value: Tetrapoda
Taxonomic_Classification:
Taxon_Rank_Name: Class
Taxon_Rank_Value: Aves
Taxonomic_Classification:
Taxon_Rank_Name: Order
Taxon_Rank_Value: Anseriformes
Taxonomic_Classification:
Taxon_Rank_Name: Family
Taxon_Rank_Value: Anatidae
Taxonomic_Classification:
Taxon_Rank_Name: Subfamily
Taxon_Rank_Value: Anserinae
Taxonomic_Classification:
Taxon_Rank_Name: Genus
Taxon_Rank_Value: Anser
Taxonomic_Classification:
Taxon_Rank_Name: Species
Taxon_Rank_Value: Anser canagicus
Applicable_Common_Name: Emperor Goose
Applicable_Common_Name: TSN: 175031
Taxonomic_Classification:
Taxon_Rank_Name: Genus
Taxon_Rank_Value: Branta
Taxonomic_Classification:
Taxon_Rank_Name: Species
Taxon_Rank_Value: Branta hutchinsii
Applicable_Common_Name: Cackling Goose
Applicable_Common_Name: TSN: 714068
Taxonomic_Classification:
Taxon_Rank_Name: Subspecies
Taxon_Rank_Value: Branta hutchinsii minima
Applicable_Common_Name: Cackling Goose
Applicable_Common_Name: TSN: 714727
Taxonomic_Classification:
Taxon_Rank_Name: no rank
Taxon_Rank_Value: Alveolata
Taxonomic_Classification:
Taxon_Rank_Name: Phylum
Taxon_Rank_Value: Apicomplexa
Taxonomic_Classification:
Taxon_Rank_Name: Class
Taxon_Rank_Value: Aconoidasida
Taxonomic_Classification:
Taxon_Rank_Name: Order
Taxon_Rank_Value: Haemosporida
Applicable_Common_Name: hematozoa
Applicable_Common_Name: avian blood parasites
Taxonomic_Classification:
Taxon_Rank_Name: Family
Taxon_Rank_Value: Leucocytozoidae
Taxonomic_Classification:
Taxon_Rank_Name: Genus
Taxon_Rank_Value: Leucocytozoon
Taxonomic_Classification:
Taxon_Rank_Name: Species
Taxon_Rank_Value: Leucocytozoon simondi
Taxonomic_Classification:
Taxon_Rank_Name: Family
Taxon_Rank_Value: Haemoproteidae
Taxonomic_Classification:
Taxon_Rank_Name: Genus
Taxon_Rank_Value: Haemoproteus
Taxonomic_Classification:
Taxon_Rank_Name: Family
Taxon_Rank_Value: Plasmodiidae
Taxonomic_Classification:
Taxon_Rank_Name: Genus
Taxon_Rank_Value: Plasmodium
Access_Constraints: None
Use_Constraints:
It is requested that the authors and the USGS Alaska Science Center be cited for any subsequent publications that reference this dataset.
Point_of_Contact:
Contact_Information:
Contact_Organization_Primary:
Contact_Organization: U.S. Geological Survey, Alaska Science Center
Contact_Address:
Address_Type: Mailing and Physical
Address: 4210 University Drive
City: Anchorage
State_or_Province: Alaska
Postal_Code: 99508
Country: USA
Contact_Voice_Telephone: 907-786-7000
Contact_Electronic_Mail_Address: ascweb@usgs.gov
Data_Set_Credit:
All DNA sequencing was performed by the: University of Wisconsin-Madison Biotechnology Center – DNA Sequencing Facility; 425 Henry Mall; Madison WI 53706. https://www.biotech.wisc.edu/services/dnaseq
Cross_Reference:
Citation_Information:
Originator: Buchheit, R.M.
Originator: Schmutz, J.A.
Originator: Reed, J.A.
Originator: Uher-Koch, B.D.
Originator: Ramey, A.M.
Publication_Date: 2021
Title:
Assessment of variation in the detection and prevalence of blood parasites among sympatrically breeding geese in western Alaska
Geospatial_Data_Presentation_Form: journal article
Series_Information:
Series_Name: Journal of Wildlife Diseases
Issue_Identification: TBD
Publication_Information:
Publication_Place: online
Publisher: Wildlife Disease Association
Other_Citation_Details:
Buchheit, R.M., Schmutz, J.A., Reed, J.A., Uher-Koch, B.D., Ramey, A.M. in prep. Assessment of variation in the detection and prevalence of blood parasites among sympatrically breeding geese in western Alaska. Journal of Wildlife Diseases TBD
Online_Linkage: https://doi.org/TBD
Data_Quality_Information:
Attribute_Accuracy:
Attribute_Accuracy_Report:
Individual sample ID's, species, age, sex, and breeding status, were cross-checked and verified to match field data. We observed sterile techniques in the handling of all DNA. All nested-PCR procedures included negative controls to check for contamination. Infection data uncertainty is related to the detection limitation of the nested-PCR procedure described in Lab Methodology. GenBank accession numbers were cross-checked to the appropriate individual samples.
Logical_Consistency_Report:
Attribute values fall within expected ranges. Blank cells indicate that no parasite haplotype was identified for the specific nested-PCR procedure.
Completeness_Report: No data were omitted and there are no missing data.
Lineage:
Methodology:
Methodology_Type: Field
Methodology_Description:
During the summers 1998 and 2014, we live-captured geese near the Manokinak River during incubation and brood rearing. Nesting females were captured with mist nets and bow nests. Flightless hatch year and adult geese were captured during brood rearing by herding birds into corrals. Whole blood was collected via jugular venipuncture, stored in Longmire buffer (Longmire et al 1988), and frozen for long-term preservation.
Methodology:
Methodology_Type: Lab
Methodology_Description:
Blood parasites were detected using molecular methods. DNA was extracted from samples using the DNeasy Blood and Tissue Kit (Qiagen, Valencia, CA). Three replicate aliquots of DNA extract were screened for hematozoa using a nested-PCR protocol (Hellgren et al 2004). A minimum of one negative control was incorporated into PCR reactions for every 24 wells. Reactions were conducted in eight-well strip tubes with individual caps that remained closed, except while loading template and reagents, to prevent cross contamination. Amplicons were visualized on 0.8% agarose gels stained with Gel Red Nucleic Acid Gel Stain 10,000× in DMSO (Biotium, Hayward, CA). All samples that had amplicons present on agarose gels, were further tested for infection by sequencing a 479-base pair target fragment of cytochrome-b mitochondrial DNA.
All samples that tested positive for hematozoa infection by nested-PCR, were sent for sequencing of the Cytochrome-b gene at the University of Wisconsin-Madison Biotechnology Center – DNA Sequencing Facility. PCR products were treated with ExoSap-IT (USB Inc., Cleveland, OH) per manufacturer instructions and not otherwise purified prior to sequencing. Amplified DNA was sequenced with the same genus-specific primers from the second stage of the nested-PCR and with BigDye Terminator version 3.1 mix (Applied Biosystems, Foster City, CA) on an Applied Biosystems 3730xl automated DNA sequencer (Applied Biosystems, Foster City, CA).
Source_Information:
Source_Citation:
Citation_Information:
Originator: Hellgren, O.
Originator: Waldenstrom, J.
Originator: Bensch, S.
Publication_Date: 2004
Title:
A new PCR assay for simultaneous studies of Leucocytozoon, Plasmodium, and Haemoproteus from avian blood
Geospatial_Data_Presentation_Form: journal article
Series_Information:
Series_Name: Journal of Parasitology
Issue_Identification: 90(4):797-802
Publication_Information:
Publication_Place: online
Publisher: BioOne
Other_Citation_Details:
Hellgren, O., Waldenstrom, J., Bensch, S. 2004. A new PCR assay for simultaneous studies of Leucocytozoon, Plasmodium, and Haemoproteus from avian blood. Journal of Parasitology 90(4):797-802. doi:10.1645/GE-184R1
Online_Linkage: https://doi.org/10.1645/GE-184R1
Type_of_Source_Media: publication
Source_Time_Period_of_Content:
Time_Period_Information:
Single_Date/Time:
Calendar_Date: 2004
Source_Currentness_Reference: publication date
Source_Citation_Abbreviation: Hellgren et al 2004
Source_Contribution:
Laboratory technique, describes PCR assay for avian blood parasite identification.
Source_Information:
Source_Citation:
Citation_Information:
Originator: Longmire, J.L.
Originator: Lewis, A.K.
Originator: Brown, N.C.
Originator: Buckingham, J.M.
Originator: Clark, L.M.
Originator: Jones, M.D.
Originator: Meincke, L.J.
Originator: Meyne, J.
Originator: Ratliff, R.L.
Originator: Ray, F.A.
Originator: Wagner, R.P.
Originator: Moyzis, R.K.
Publication_Date: 1988
Title:
Isolation and molecular characterization of a highly polymorphic centromeric tandem repeat in the family Falconidae
Geospatial_Data_Presentation_Form: journal article
Series_Information:
Series_Name: Genomics
Issue_Identification: 2(1):14–24
Publication_Information:
Publication_Place: online
Publisher: Elsevier
Other_Citation_Details:
Longmire, J.L., A.K. Lewis, N.C. Brown, J.M. Buckingham, L.M. Clark, M.D. Jones, L.J. Meincke, J. Meyne, R.L. Ratliff, F.A. Ray, R.P. Wagner, R.K. Moyzis. 1988. Isolation and molecular characterization of a highly polymorphic centromeric tandem repeat in the family Falconidae. Genomics 2(1):14–24 doi:10.1016/0888-7543(88)90104-8
Online_Linkage: https://doi.org/10.1016/0888-7543(88)90104-8
Type_of_Source_Media: publication
Source_Time_Period_of_Content:
Time_Period_Information:
Single_Date/Time:
Calendar_Date: 1998
Source_Currentness_Reference: publication date
Source_Citation_Abbreviation: Longmire et al 1988
Source_Contribution: Laboratory technique, describes blood preservation buffer.
Source_Information:
Source_Citation:
Citation_Information:
Originator: Altschul, S.F.
Originator: Gish, W.
Originator: Miller, W.
Originator: Myers, E.W.
Originator: Lipman, D.J.
Publication_Date: 1990
Title: Basic local alignment search tool
Geospatial_Data_Presentation_Form: journal article
Series_Information:
Series_Name: Journal of Molecular Biology
Issue_Identification: 215(3):403-410
Publication_Information:
Publication_Place: online
Publisher: Elsevier
Other_Citation_Details:
Altschul, S.F., Gish, W., Miller, W., Myers, E.W., Lipman, D.J. 1990. Basic local alignment search tool. Journal of Molecular Biology. 215(3):403-410. doi:10.1016/S0022-2836(05)80360-2
Online_Linkage: https://doi.org/10.1016/S0022-2836(05)80360-2
Type_of_Source_Media: publication
Source_Time_Period_of_Content:
Time_Period_Information:
Single_Date/Time:
Calendar_Date: 1990
Source_Currentness_Reference: publication date
Source_Citation_Abbreviation: Altschul et al 1990
Source_Contribution:
Reference for the use of the BLAST (Basic local alignment search tool) function in searching alignment of nucleotide sequences.
Source_Information:
Source_Citation:
Citation_Information:
Originator: National Center for Biotechnology Information (NCBI)
Publication_Date: Unknown
Title: GenBank
Geospatial_Data_Presentation_Form: database
Online_Linkage: https://www.ncbi.nlm.nih.gov/genbank/
Type_of_Source_Media: digital database file
Source_Time_Period_of_Content:
Time_Period_Information:
Single_Date/Time:
Calendar_Date: 2017
Source_Currentness_Reference: observed
Source_Citation_Abbreviation: NCBI GenBank
Source_Contribution:
DNA sequences obtained in the current study were queried against all known hematozoa sequences in GenBank using the nucleotide BLAST function (Altschul et al 1990) and determined to be positive if there was >90% consensus with one or more entries in GenBank
Process_Step:
Process_Description:
Sequencher version 5.1 (Gene Codes Corp., Ann Arbor, MI) was used to examine and align all sequence data. International Union of Pure and Applied Chemistry (IUPAC) ambiguity codes were assigned to base pairs with uncertain or multiple nucleic acid identity. Blood parasite infections were confirmed and assigned to a genus (Leucocytozoon, Haemoproteus, or Plasmodium) when a bi-directional consensus sequence of target mtDNA was matched to one of the three parasite genera with >90% identity. Sequences were matched using the nucleotide BLAST function (Altschul et al 1990) for comparison with sequences on NCBI GenBank. Sequences that were not bi-directionally aligned and sequences with <90% identity were considered negative to reduce the possible occurrence of false positives.
Process_Date: Unknown
Process_Step:
Process_Description:
Each unique DNA sequence (haplotype) isolated in this study has been submitted to NCBI GenBank and assigned a GenBank Accession Number which may be used to retrieve detailed sequence information from the NCBI GenBank database https://www.ncbi.nlm.nih.gov/genbank
Process_Date: Unknown
Spatial_Data_Organization_Information:
Indirect_Spatial_Reference:
Blood samples were collected from geese near the mouth of the Manokinak River in the Yukon-Kuskokwim Delta National Wildlife Refuge (approximately 61.15 N 165.15 W)
Entity_and_Attribute_Information:
Detailed_Description:
Entity_Type:
Entity_Type_Label: goose_bloodParasites_YKD_reed_1998_2014.csv
Entity_Type_Definition:
Table with sample and blood parasite infection data from Emperor geese and Cackling geese in western Alaska, 1998-2014. Presented in a Comma Separated Value (CSV) formatted table.
Entity_Type_Definition_Source: Author defined
Attribute:
Attribute_Label: Sample_ID
Attribute_Definition:
Unique identifier of each blood sample included in the analysis.
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Unrepresentable_Domain:
Unique identifier composed of: species code (EMGO = Emperor goose CACG = Cackling goose), location code (AK = Alaska), two-digit year (1998, 2014), and a sequential sample number.
Attribute:
Attribute_Label: Band_ID
Attribute_Definition:
Unique field identification code, used to mark individual birds in the field such as a coded plastic neck collar, tarsal flag, or the final 5 digits of the metal leg band issued by the USGS Bird Banding Lab. Blank cells indicate the individual did not have a band.
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Unrepresentable_Domain: Unique field bird identifier
Attribute:
Attribute_Label: Species
Attribute_Definition:
Bird species. Taxonomic details (including scientific names) are provided in the Taxonomy section of this metadata record.
Attribute_Definition_Source: American Ornithological Society
Attribute_Domain_Values:
Unrepresentable_Domain: Common name of bird species
Attribute:
Attribute_Label: AgeClass
Attribute_Definition: Age class of sampled goose.
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: AHY
Enumerated_Domain_Value_Definition:
After hatch year. A bird known to have hatched before the calendar year of banding; year of hatch otherwise unknown.
Enumerated_Domain_Value_Definition_Source: Author defined
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: HY
Enumerated_Domain_Value_Definition:
Hatch year. A bird known to have hatched during the calendar year in which it was banded.
Enumerated_Domain_Value_Definition_Source: Author defined
Attribute:
Attribute_Label: Sex
Attribute_Definition: Sex of the sampled goose.
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: F
Enumerated_Domain_Value_Definition: Female
Enumerated_Domain_Value_Definition_Source: Author defined
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: M
Enumerated_Domain_Value_Definition: Male
Enumerated_Domain_Value_Definition_Source: Author defined
Attribute:
Attribute_Label: BreedingStage
Attribute_Definition: Breeding stage of goose when captured.
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: Nesting
Enumerated_Domain_Value_Definition: Adult female captured while nesting.
Enumerated_Domain_Value_Definition_Source: Author defined
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: Brood Drives
Enumerated_Domain_Value_Definition: Adult or hatch year goose captured during brood drive.
Enumerated_Domain_Value_Definition_Source: Author defined
Attribute:
Attribute_Label: L1 through P3
Attribute_Definition:
These 9 columns present infection results for three replicate tests (indicated by the number) for three blood parasite genera (L = Leucocytozoon, H = Haemoproteus, P = Plasmodium).
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: 0
Enumerated_Domain_Value_Definition:
Negative result: nested-PCR test that resulted in no product, single-stranded sequences, or products that could not be assigned to blood parasite genera via nucleotide BLAST.
Enumerated_Domain_Value_Definition_Source: Author defined
Attribute_Domain_Values:
Enumerated_Domain:
Enumerated_Domain_Value: 1
Enumerated_Domain_Value_Definition:
Positive result: nested-PCR resulted in double-stranded target mitochondrial DNA (mtDNA) that was verified through genetic sequencing and assigned to a blood parasite genus (with an identity >90%) by nucleotide BLAST.
Enumerated_Domain_Value_Definition_Source: Author defined
Attribute:
Attribute_Label: L1_GenBank through P3_GenBank
Attribute_Definition:
These 9 columns present the GenBank Accession Numbers of the cytochrome b (cyt b) mitochondrial DNA (mtDNA) target fragment isolated from infected samples (see the corresponding infection results attributes). Blank cells indicate negative results from the nested-PCR.
The accession number is a unique identifier assigned by the National Center for Biotechnology Information (NCBI) for nucleotide sequences. Each unique DNA sequence (haplotype) isolated in this study has been assigned an accession number which may be used to retrieve detailed sequence information from the NCBI GenBank database https://www.ncbi.nlm.nih.gov/genbank
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:
Unrepresentable_Domain: GenBank Accession Number. A unique identifier assigned by NCBI
Distribution_Information:
Distributor:
Contact_Information:
Contact_Organization_Primary:
Contact_Organization: U.S. Geological Survey, Alaska Science Center
Contact_Address:
Address_Type: Mailing and Physical
Address: 4210 University Drive
City: Anchorage
State_or_Province: Alaska
Postal_Code: 99508
Country: USA
Contact_Voice_Telephone: 907-786-7000
Contact_Electronic_Mail_Address: ascweb@usgs.gov
Resource_Description:
The U.S. Geological Survey, Alaska Science Center is the authoritative source and distributor of these data.
Distribution_Liability:
Unless otherwise stated, all data, metadata and related materials are considered to satisfy the quality standards relative to the purpose for which the data were collected. Although these data and associated metadata have been reviewed for accuracy and completeness and approved for release by the U.S. Geological Survey, no warranty expressed or implied is made regarding the display or utility of the data for other purposes or on all computer systems, nor shall the act of distribution constitute any such warranty. Any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the U.S. Government.
Standard_Order_Process:
Digital_Form:
Digital_Transfer_Information:
Format_Name: CSV
Format_Information_Content:
Data are distributed in a Zip package containing data in CSV format and FGDC metadata in XML and HTML formats.
File_Decompression_Technique:
Compression applied by the 7-Zip utility using default compression (5). The file can be decompressed and unpacked by open source or commercially available unzip tools.
Digital_Transfer_Option:
Online_Option:
Computer_Contact_Information:
Network_Address:
Network_Resource_Name: https://doi.org/10.5066/P9F7LD2I
Fees: None
Metadata_Reference_Information:
Metadata_Date: 20210310
Metadata_Contact:
Contact_Information:
Contact_Organization_Primary:
Contact_Organization: U.S. Geological Survey, Alaska Science Center
Contact_Address:
Address_Type: Mailing and Physical
Address: 4210 University Drive
City: Anchorage
State_or_Province: Alaska
Postal_Code: 99508
Country: USA
Contact_Voice_Telephone: 907-786-7000
Contact_Electronic_Mail_Address: ascweb@usgs.gov
Metadata_Standard_Name:
FGDC Biological Data Profile of the Content Standard for Digital Geospatial Metadata
Metadata_Standard_Version: FGDC-STD-001.1-1999

Generated by mp version 2.9.50 on Fri Mar 5 16:22:33 2021