Genetic Data From Nesting Steller’s Eiders (Polysticta stelleri), Near Utqiaġvik, Alaska, 1995-2016

Identification_Information:

Citation:

Citation_Information:

Originator: Talbot, S.L. (ORCID: 0000-0002-3312-7214)
Originator: Sage, G.K. (ORCID: 0000-0003-1431-2286)
Originator: Fales, K.R. (ORCID: 0000-0001-6436-9956)
Originator: Pierson, B.J. (ORCID: 0000-0001-8233-874X)
Publication_Date: 20200427
Title:

Geospatial_Data_Presentation_Form: tabular digital data
Publication_Information:

Publication_Place: Anchorage, Alaska
Publisher: U.S. Geological Survey, Alaska Science Center

Other_Citation_Details:

Suggested Citation: Talbot, S.L., Sage, G.K., Fales, K.R., and Pierson, B.J., 2020, Genetic data from nesting Steller’s Eiders (Polysticta stelleri), near Utqiaġvik, Alaska, 1995-2016: U.S. Geological Survey data release: https://doi.org/10.5066/P967DZ5L

Online_Linkage: https://doi.org/10.5066/P967DZ5L
Larger_Work_Citation:

Citation_Information:

Originator: U.S. Geological Survey, Alaska Science Center
Publication_Date: 2000
Title:

Developing and Applying Molecular Tools to Natural Resource Problems in Alaska

Geospatial_Data_Presentation_Form: website
Series_Information:

Series_Name: Alaska Science Portal
Issue_Identification: 378

Publication_Information:

Publication_Place: Anchorage, Alaska
Publisher: U.S. Geological Survey, Alaska Science Center

Other_Citation_Details:

This is a link to the broader USGS Alaska Science Center research project supported by these data. Users will find a description of the research project and links to associated reports, publications, and data products.

Online_Linkage: https://alaska.usgs.gov/portal/project.php?project_id=378

Description:

Abstract:

This data set is comprised of one table with Steller's Eider sample information and allele sizes for 6 microsatellite loci.

Purpose:

Data were collected to assess breeding site fidelity and philopatry.

Time_Period_of_Content:

Time_Period_Information:

Range_of_Dates/Times:

Beginning_Date: 1993
Ending_Date: 2017

Currentness_Reference: observed

Status:

Progress: Complete
Maintenance_and_Update_Frequency: None planned

Spatial_Domain:

Description_of_Geographic_Extent:

The sampling area includes tundra areas surrounding Utqiaġvik, Alaska.

Bounding_Coordinates:

West_Bounding_Coordinate: -156.96
East_Bounding_Coordinate: -156.20
North_Bounding_Coordinate: 71.35
South_Bounding_Coordinate: 71.19

Keywords:

Theme:

Theme_Keyword_Thesaurus: USGS Metadata Identifier
Theme_Keyword: USGS:ASC266

Theme:

Theme_Keyword_Thesaurus: ISO 19115 Topic Category
Theme_Keyword: biota

Theme:

Theme_Keyword_Thesaurus: NASA GCMD Earth Science Thesaurus
Theme_Keyword: Animals/Vertebrates
Theme_Keyword: Birds
Theme_Keyword: Ducks/Geese/Swans

Theme:

Theme_Keyword_Thesaurus: USGS CSA Biocomplexity Thesaurus
Theme_Keyword: Waterfowl
Theme_Keyword: Migratory birds
Theme_Keyword: Threatened species
Theme_Keyword: Site Fidelity
Theme_Keyword: Population Genetics

Theme:

Theme_Keyword_Thesaurus: USGS Thesaurus
Theme_Keyword: Wildlife
Theme_Keyword: Tundra ecosystems
Theme_Keyword: Migratory Species
Theme_Keyword: Genetics
Theme_Keyword: Polymerase chain reaction

Theme:

Theme_Keyword_Thesaurus: MeSH-National Library of Medicine
Theme_Keyword: Genetic markers
Theme_Keyword: Microsatellite Repeats
Theme_Keyword: DNA
Theme_Keyword: Alleles
Theme_Keyword: Genotype

Theme:

Theme_Keyword_Thesaurus: None
Theme_Keyword: Sfiµ3
Theme_Keyword: Sfiµ4
Theme_Keyword: Sfiµ10
Theme_Keyword: Sfiµ11
Theme_Keyword: Bcaµ11
Theme_Keyword: Smo10
Theme_Keyword: Steller's Eider

Place:

Place_Keyword_Thesaurus: USGS Geographic Names Information System
Place_Keyword: Alaska
Place_Keyword: Utqiaġvik
Place_Keyword: Utqiagvik

Taxonomy:

Keywords/Taxon:

Taxonomic_Keyword_Thesaurus: None
Taxonomic_Keywords: Polysticta stelleri

Taxonomic_System:

Classification_System/Authority:

Classification_System_Citation:

Citation_Information:

Originator: ITIS Integrated Taxonomic Information System
Publication_Date: Unknown
Title: ITIS Integrated Taxonomic Information System
Geospatial_Data_Presentation_Form: database
Series_Information:

Series_Name: Taxonomic Serial Number
Issue_Identification: TSN 175153

Publication_Information:

Publication_Place: online
Publisher: ITIS-North America

Other_Citation_Details:

Taxonomic details for Steller's Eider retrieved January 10, 2020, from the Integrated Taxonomic Information System on-line database https://www.itis.gov

Online_Linkage: https://www.itis.gov

Taxonomic_Procedures:

Species were identified by skilled observers in the field based on general appearance of feathers and plumage, behavior, and vocalization.

Taxonomic_Classification:

Taxon_Rank_Name: Kingdom
Taxon_Rank_Value: Animalia
Taxonomic_Classification:

Taxon_Rank_Name: Subkingdom
Taxon_Rank_Value: Bilateria
Taxonomic_Classification:

Taxon_Rank_Name: Infrakingdom
Taxon_Rank_Value: Deuterostomia
Taxonomic_Classification:

Taxon_Rank_Name: Phylum
Taxon_Rank_Value: Chordata
Taxonomic_Classification:

Taxon_Rank_Name: Subphylum
Taxon_Rank_Value: Vertebrata
Taxonomic_Classification:

Taxon_Rank_Name: Infraphylum
Taxon_Rank_Value: Gnathostomata
Taxonomic_Classification:

Taxon_Rank_Name: Superclass
Taxon_Rank_Value: Tetrapoda
Taxonomic_Classification:

Taxon_Rank_Name: Class
Taxon_Rank_Value: Aves
Taxonomic_Classification:

Taxon_Rank_Name: Order
Taxon_Rank_Value: Anseriformes
Taxonomic_Classification:

Taxon_Rank_Name: Family
Taxon_Rank_Value: Anatidae
Taxonomic_Classification:

Taxon_Rank_Name: Subfamily
Taxon_Rank_Value: Anatinae
Taxonomic_Classification:

Taxon_Rank_Name: Genus
Taxon_Rank_Value: Polysticta
Taxonomic_Classification:

Taxon_Rank_Name: Species
Taxon_Rank_Value: Polysticta stelleri
Applicable_Common_Name: Steller's Eider
Applicable_Common_Name: TSN: 175153

Access_Constraints: None
Use_Constraints:

It is requested that the authors and the USGS Alaska Science Center be cited for any subsequent publications that reference this dataset.

Point_of_Contact:

Contact_Information:

Contact_Organization_Primary:

Contact_Organization: U.S. Geological Survey, Alaska Science Center

Contact_Address:

Address_Type: mailing and physical
Address: 4210 University Drive
City: Anchorage
State_or_Province: Alaska
Postal_Code: 99508
Country: USA

Contact_Voice_Telephone: 907-786-7000
Contact_Electronic_Mail_Address: ascweb@usgs.gov

Cross_Reference:

Citation_Information:

Originator: Safine, D.E.
Originator: Lindberg, M.S.
Originator: Martin, K.H.
Originator: Talbot, S.L. (ORCID: 0000-0002-3312-7214)
Originator: Swem, T.R.
Originator: Pearce, J.M. (ORCID: 0000-0002-8503-5485)
Originator: Stellrecht, N.C.
Originator: Sage, G.K. (ORCID: 0000-0003-1431-2286)
Originator: Riddle, A.E.
Originator: Fales, K. (ORCID: 0000-0001-6436-9956)
Originator: Hollmén, T.E.
Publication_Date: 2020
Title:

Use of genetic mark−recapture to estimate breeding site fidelity and philopatry in a threatened sea duck population, Alaska-breeding Steller’s eiders

Geospatial_Data_Presentation_Form: journal article
Series_Information:

Series_Name: Endangered Species Research
Issue_Identification: 41:349-360

Publication_Information:

Publication_Place: online
Publisher: Inter-Research Science Publisher

Other_Citation_Details:

Safine, D.E., Lindberg, M.S., Martin, K.H., Talbot, S.L., Swem, T.R., Pearce, J.M., Stellrecht, N.C., Sage, G.K., Riddle, A.E., Fales, K., and Hollmén, T.E. 2020. Use of genetic mark−recapture to estimate breeding site fidelity and philopatry in a threatened sea duck population, Alaska-breeding Steller’s eiders. Endangered Species Research 41:349-360 doi:10.3354/esr01026

Online_Linkage: https://doi.org/10.3354/esr01026

Data_Quality_Information:

Attribute_Accuracy:

Attribute_Accuracy_Report:

Sampling information provided by collectors was cross-checked and verified to match the accompanying data. All laboratory techniques were performed using sterile procedures. While DNA extractions from single feathers cannot be duplicated due to low yields of initial extractions, quality control (QC) for eggshell membranes included a minimum of 10% re-extraction and PCR replication for each locus. Additionally, for feathers, in many cases duplicate feathers from the same nest were available and provided internal quality control described in Safine et al. (2020). All duplicate characterizations of the same individual (eggshell membrane duplicates and paired blood/feather individuals) resulted in concordant data. Species identification by collectors were informally verified by confirming genetic "signatures" (e.g. microsatellite size) fell within expected values for the species.

Logical_Consistency_Report:

For microsatellite loci, reported allele sizes were reviewed to confirm they conform to expected distributions (e.g. dinucleotide repeats with 1 base pair differences were reviewed on gel images with laboratory personnel, gaps in allele sizes were reviewed, and single occurrences of an allele size were scrutinized). Any inconsistencies in allele scores were resolved with additional replicates when possible. Tests for null alleles and allelic dropout were implemented in MicroChecker (van Oosterhout et al 2004) and GENECAP (Wilberg and Dreher 2004).

Completeness_Report:

Blank cells are described for each attribute. Eggshell membranes determined to be male were dropped from the study and do not have microsatellite data.

Lineage:

Methodology:

Methodology_Type: Lab
Methodology_Description:

DNA was extracted from blood and membranes by a modified salt extraction technique (Medrano et al 1990; Sonsthagen et al 2004) and from feathers following Talbot et al (2011). Ten individuals had both blood and feather samples to test robustness of the chosen suite of markers for use in feathers. Two to five feathers from 224 nests were extracted individually (i.e. not pooled in case of multiple individuals, but presumed to be from a single adult female). Eggshell membranes from 75 nests were extracted.

Methodology:

Methodology_Type: Lab
Methodology_Description:

Polymerase chain reaction (PCR) labelling of loci and scoring of microsatellite and sexing (CHD) loci followed Handel et al 2006 and reaction conditions and thermocycler parameters followed Safine et al (2020). Microsatellite alleles were resolved on a 25 cm 6% polyacrylamide gel and CHD was resolved on an 18 cm 6% polyacrylamide gel using a LI-COR (IR 4200) automated DNA sequencer.

Source_Information:

Source_Citation:

Citation_Information:

Originator: Handel, C.M.
Originator: Pajot, L.M.
Originator: Talbot, S.L
Originator: Sage, G.K.
Publication_Date: 2006
Title:

Use of buccal swabs for sampling DNA from nestling and adult birds

Geospatial_Data_Presentation_Form: journal article
Series_Information:

Series_Name: Wildlife Society Bulletin
Issue_Identification: 34:1094-1100

Publication_Information:

Publication_Place: online
Publisher: The Wildlife Society

Other_Citation_Details:

Handel, C.M., Pajot, L.M., Talbot, S.L., Sage, G.K. 2006. Use of buccal swabs for sampling DNA from nestling and adult birds. Wildlife Society Bulletin 34:1094-1100 doi:10.2193/0091-7648(2006)34[1094:UOBSFS]2.0.CO;2

Online_Linkage: https://doi.org/10.2193/0091-7648(2006)34[1094:UOBSFS]2.0.CO;2

Type_of_Source_Media: publication
Source_Time_Period_of_Content:

Time_Period_Information:

Single_Date/Time:

Calendar_Date: 2006

Source_Currentness_Reference: publication date

Source_Citation_Abbreviation: Handel et al 2006
Source_Contribution:

Laboratory Technique Citation: describes polymerase chain reaction (PCR) amplification and scoring of microsatellites.

Source_Information:

Source_Citation:

Citation_Information:

Originator: Medrano, J.F.
Originator: Aasen, E.
Originator: Sharrow, L.
Publication_Date: 1990
Title: DNA extraction from nucleated red blood cells
Geospatial_Data_Presentation_Form: journal article
Series_Information:

Series_Name: Biotechniques
Issue_Identification: 8(1):43

Publication_Information:

Publication_Place: online
Publisher: Future Science, Ltd.

Other_Citation_Details:

Medrano, J.F., Aasen, E., Sharrow, L. 1990. DNA extraction from nucleated red blood cells. Biotechniques 8(1):43

Type_of_Source_Media: publication
Source_Time_Period_of_Content:

Time_Period_Information:

Single_Date/Time:

Calendar_Date: 1990

Source_Currentness_Reference: publication date

Source_Citation_Abbreviation: Medrano et al 1990
Source_Contribution:

Laboratory Technique Citation: describes DNA extraction technique.

Source_Information:

Source_Citation:

Citation_Information:

Use of genetic mark−recapture to estimate breeding site fidelity and philopatry in a threatened sea duck population, Alaska-breeding Steller’s eiders

Geospatial_Data_Presentation_Form: journal article
Series_Information:

Series_Name: Endangered Species Research
Issue_Identification: 41:349-360

Publication_Information:

Publication_Place: online
Publisher: Inter-Research Science Publisher

Other_Citation_Details:

Online_Linkage: https://doi.org/10.3354/esr01026

Type_of_Source_Media: publication
Source_Time_Period_of_Content:

Time_Period_Information:

Single_Date/Time:

Calendar_Date: 20200409

Source_Currentness_Reference: publication date

Source_Citation_Abbreviation: Safine et al 2020
Source_Contribution:

Laboratory Technique Citation: supplementary material describes DNA extraction, polymerase chain reaction (PCR) amplification and electrophoresis and scoring of microsatellite protocols as well as the detailed data quality control measures for this data release.

Source_Information:

Source_Citation:

Citation_Information:

Originator: Sonsthagen, S.A.
Originator: Talbot, S.L.
Originator: White, C.M.
Publication_Date: 2004
Title:

Gene flow and genetic characterization of Northern Goshawks breeding in Utah.

Geospatial_Data_Presentation_Form: journal article
Series_Information:

Series_Name: Condor
Issue_Identification: 106(4):826–836

Publication_Information:

Publication_Place: Oxford, UK
Publisher: Oxford University Press

Other_Citation_Details:

Sonsthagen, S.A., Talbot, S.L., White, C.M. 2004. Gene flow and genetic characterization of Northern Goshawks breeding in Utah. Condor 106(4):826–836.

Online_Linkage: http://www.jstor.org/stable/3247787

Type_of_Source_Media: publication
Source_Time_Period_of_Content:

Time_Period_Information:

Single_Date/Time:

Calendar_Date: 2004

Source_Currentness_Reference: publication date

Source_Citation_Abbreviation: Sonsthagen et al 2004
Source_Contribution:

Laboratory Technique Citation: describes DNA extraction, polymerase chain reaction (PCR) amplification and electrophoresis and scoring of microsatellite protocols.

Source_Information:

Source_Citation:

Citation_Information:

Originator: Talbot, S.L.
Originator: Palmer, A.G.
Originator: Sage, G.K.
Originator: Sonsthagen, S.A.
Originator: Swem, T.
Originator: Brimm, D.
Originator: White, C.M.
Publication_Date: 2011
Title:

Lack of genetic polymorphism among peregrine falcons Falco peregrinus of Fiji

Geospatial_Data_Presentation_Form: journal article
Series_Information:

Series_Name: Journal of Avian Biology
Issue_Identification: 42:415-428

Publication_Information:

Publication_Place: online
Publisher: John Wiley and Sons

Other_Citation_Details:

Talbot, S.L., Palmer, A.G., Sage, G.K., Sonsthagen, S.A., Swem, T., Brimm, D., White, C.M. 2011. Lack of genetic polymorphism among peregrine falcons Falco peregrinus of Fiji. Journal of Avian Biology 42:415-428 doi:j.1600-048X.2011.05280.x

Online_Linkage: https://doi.org/10.1111/j.1600-048X.2011.05280.x

Type_of_Source_Media: publication
Source_Time_Period_of_Content:

Time_Period_Information:

Single_Date/Time:

Calendar_Date: 2011

Source_Currentness_Reference: publication date

Source_Citation_Abbreviation: Talbot et al 2011
Source_Contribution:

Laboratory Technique Citation: describes DNA extraction from feathers.

Source_Information:

Source_Citation:

Citation_Information:

Originator: van Oosterhout, C.
Originator: Hutchinson, W.F.
Originator: Wills, D.P.M.
Originator: Shipley, P.
Publication_Date: 2004
Title:

MICRO-CHECKER: software for identifying and correcting genotyping errors in microsatellite data

Geospatial_Data_Presentation_Form: journal article
Series_Information:

Series_Name: Molecular Ecology Notes
Issue_Identification: 4(3):535-538

Publication_Information:

Publication_Place: online
Publisher: Wiley Online Library

Other_Citation_Details:

van Oosterhout, C., Hutchinson, W.F., Wills, D.P.M., Shipley, P. 2004. MICRO-CHECKER: software for identifying and correcting genotyping errors in microsatellite data. Molecular Ecology Notes 4(3):535-538 doi:10.1111/j.1471-8286.2004.00684.x

Online_Linkage: https://doi.org/10.1111/j.1471-8286.2004.00684.x

Type_of_Source_Media: publication
Source_Time_Period_of_Content:

Time_Period_Information:

Single_Date/Time:

Calendar_Date: 2004

Source_Currentness_Reference: publication date

Source_Citation_Abbreviation: van Oosterhout et al 2004
Source_Contribution:

Laboratory Technique Citation: describes tool used to identify potential scoring errors and null alleles of microsatellite loci.

Source_Information:

Source_Citation:

Citation_Information:

Originator: Talbot, S.L.
Originator: Pierson, B.J.
Originator: Sage, G.K.
Publication_Date: 2020
Title:

Dictionary of Microsatellite Loci from the U.S. Geological Survey, Alaska Science Center, Molecular Ecology Laboratory, 1993-present

Geospatial_Data_Presentation_Form: tabular digital data
Other_Citation_Details:

Talbot, S.L., Pierson, B.J., and Sage, G.K., 2020, Microsatellite genetic screening data from the U.S. Geological Survey, Alaska Science Center, Molecular Ecology Laboratory, 1993-present (ver. 1.0, April 2020): U.S. Geological Survey data release, https://doi.org/10.5066/P9GJEX8Y

Online_Linkage: https://doi.org/10.5066/P9GJEX8Y

Type_of_Source_Media: digital database file
Source_Time_Period_of_Content:

Time_Period_Information:

Single_Date/Time:

Calendar_Date: 2020

Source_Currentness_Reference: publication date

Source_Citation_Abbreviation: MEL Microsatellite Dictionary
Source_Contribution:

Look-up File: microsatellite locus names and citations to sources for additional information about the locus primers.

Source_Information:

Source_Citation:

Citation_Information:

Originator: Waits, L.P.
Originator: Paetkau, D.
Publication_Date: 2005
Title:

Noninvasive genetic sampling tools for wildlife biologists: a review of application and recommendations for accurate data collection

Geospatial_Data_Presentation_Form: journal article
Series_Information:

Series_Name: Journal of Wildlife Management
Issue_Identification: 69(4):1419–1433

Publication_Information:

Publication_Place: online
Publisher: The Wildlife Society

Other_Citation_Details:

Waits, L.P., Paetkau. D. 2005. Noninvasive genetic sampling tools for wildlife biologists: a review of application and recommendations for accurate data collection. Journal of Wildlife Management 69:1419–1433 doi:10.2193/0022-541X(2005)69[1419:NGSTFW]2.0.CO;2

Online_Linkage: https://doi.org/10.2193/0022-541X(2005)69[1419:NGSTFW]2.0.CO;2

Type_of_Source_Media: publication
Source_Time_Period_of_Content:

Time_Period_Information:

Single_Date/Time:

Calendar_Date: 2005

Source_Currentness_Reference: publication date

Source_Citation_Abbreviation: Waits and Paetkau 2005
Source_Contribution:

Process reference: strategies for minimizing error in studies with low-yield DNA materials.

Source_Information:

Source_Citation:

Citation_Information:

Originator: Wilberg, M.J.
Originator: Dreher, B.P.
Publication_Date: 2004
Title:

GENECAP: A program for analysis of multilocus genotype data for non-invasive sampling and capture-recapture population estimation

Geospatial_Data_Presentation_Form: journal article
Series_Information:

Series_Name: Molecular Ecology Notes
Issue_Identification: 4:783–785

Publication_Information:

Publication_Place: online
Publisher: John Wiley and Sons

Other_Citation_Details:

Wilberg, M.J., Dreher, B.P. 2004. GENECAP: A program for analysis of multilocus genotype data for non-invasive sampling and capture-recapture population estimation. Molecular Ecology Notes 4:783–785 doi:10.1111/j.1471-8286.2004.00797.x

Online_Linkage: https://doi.org/10.1111/j.1471-8286.2004.00797.x

Type_of_Source_Media: publication
Source_Time_Period_of_Content:

Time_Period_Information:

Single_Date/Time:

Calendar_Date: 2004

Source_Currentness_Reference: publication date

Source_Citation_Abbreviation: Wilberg and Dreher 2004
Source_Contribution:

Logic and process reference: GENECAP is used to detect genotyping errors and to calculate probabilities of identity.

Process_Step:

Process_Description:

Initial microsatellite allele sizes were determined by scoring based on PCR product size relative to an M13 DNA sequence ladder using Gene Profiler 4.05 (Scanalytics, Inc., Fairfax, VA). Subsequent gels included these known size individuals from which relative sizes of unknown individuals were assigned using Gene Profiler 4.05. For this reason, these microsatellite data cannot be combined with data generated outside the USGS Alaska Science Center without molecular size calibration between laboratories. Strict review of pooled data prior to pooling into a single composite genotype for one individual are described in Safine et al (2020) and followed recommendations set forth in Waits and Paetkau (2005). Tests for null alleles and allelic dropout were implemented in MicroChecker (van Oosterhout et al 2004) and again downstream processing in GENECAP (Wilberg and Dreher 2004).

Process_Date: Unknown

Spatial_Data_Organization_Information:

Indirect_Spatial_Reference:

The sampling location has been described by the corresponding entry in the Geographic Names Information System (GNIS).

Direct_Spatial_Reference_Method: Point

Spatial_Reference_Information:

Horizontal_Coordinate_System_Definition:

Geographic:

Latitude_Resolution: 0.01
Longitude_Resolution: 0.01
Geographic_Coordinate_Units: Decimal degrees

Geodetic_Model:

Horizontal_Datum_Name: World Geodetic System of 1984
Ellipsoid_Name: World Geodetic System of 1984
Semi-major_Axis: 6378137
Denominator_of_Flattening_Ratio: 298.257223563

Entity_and_Attribute_Information:

Detailed_Description:

Entity_Type:

Entity_Type_Label: stellersEider_microsatellite_talbot_utqiagvik
Entity_Type_Definition:

Table with sample information, nest identification, molecularly-determined sex, and allele scores (in base pairs) for 6 autosomal microsatellite loci. Presented in a Comma Separated Value (CSV) formatted table.

Locus names and order of presentation: Sfiµ3, Sfiµ4, Sfiµ10, Sfiµ11, Bcaµ11, Smo10

Entity_Type_Definition_Source: Author defined

Attribute:

Attribute_Label: Sample_ID
Attribute_Definition: Unique laboratory label for each individual characterized.
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:

Unrepresentable_Domain:

Unique alphanumeric label assigned by the laboratory for materials originating from a single individual.

Attribute:

Attribute_Label: Type
Attribute_Definition: Source of DNA used in the characterization.
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:

Enumerated_Domain:

Enumerated_Domain_Value: membrane
Enumerated_Domain_Value_Definition:

DNA was extracted from an eggshell membrane found within a nest.

Enumerated_Domain_Value_Definition_Source: Author defined

Attribute_Domain_Values:

Enumerated_Domain:

Enumerated_Domain_Value: feather
Enumerated_Domain_Value_Definition: DNA was extracted from contour feathers found within a nest.
Enumerated_Domain_Value_Definition_Source: Author defined

Attribute_Domain_Values:

Enumerated_Domain:

Enumerated_Domain_Value: blood
Enumerated_Domain_Value_Definition: DNA was extracted from blood drawn from a live capture.
Enumerated_Domain_Value_Definition_Source: Author defined

Attribute:

Attribute_Label: Nest
Attribute_Definition:

Label or labels assigned to all materials (blood, feathers and/or membranes) from a single nest. Empty cells indicate samples collected away from a nest or that nest label was not provided for the sample. Samples including a "?" indicate a certain nest ID was not possible due to mixture of membranes from multiple nests during sample processing (STEI 15-HCC01?) or due to incomplete sampling information (13-DEG027?).

Attribute_Definition_Source: Author defined
Attribute_Domain_Values:

Unrepresentable_Domain: Unique (within year of sampling) alphanumeric nest label.

Attribute:

Attribute_Label: Sampling_Year
Attribute_Definition: Year sample was collected from the field.
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:

Range_Domain:

Range_Domain_Minimum: 1993
Range_Domain_Maximum: 2016
Attribute_Units_of_Measure: Year (YYYY)

Attribute:

Attribute_Label: Sex
Attribute_Definition:

Sex determined via DNA-based amplification of Chromo helicase DNA-binding (CHD) gene. Blank cells indicate sex could not be determined for membranes, or in the case of contour feathers and blood, was not determined genetically since females are easily identified in hand and males are not present on nest.

Attribute_Definition_Source: Author defined
Attribute_Domain_Values:

Enumerated_Domain:

Enumerated_Domain_Value: F
Enumerated_Domain_Value_Definition: Sample determined to be female.
Enumerated_Domain_Value_Definition_Source: Author defined

Attribute_Domain_Values:

Enumerated_Domain:

Enumerated_Domain_Value: M
Enumerated_Domain_Value_Definition: Sample determined to be male.
Enumerated_Domain_Value_Definition_Source: Author defined

Attribute:

Attribute_Label: Sfiµ3_allele_1 through Smol0_allele_2
Attribute_Definition:

These 12 columns represent 6 autosomal microsatellite loci. Each microsatellite locus genotype is represented by two adjacent columns (i.e., allele_1 and allele_2) each containing one allele score. Blank cells indicate data were not collected for the sample. Microsatellite data for membranes for which a genetically assigned sex was male or could not be determined are not included in this release.

Locus names and order of presentation: Sfiµ3, Sfiµ4, Sfiµ10, Sfiµ11, Bcaµ11, Smo10

These microsatellite data cannot be combined with data generated outside the Alaska Science Center without molecular size calibration between laboratories.

Details and full citations for microsatellite loci are available at https://doi.org/10.5066/P9GJEX8Y

Attribute_Definition_Source: Author defined
Attribute_Domain_Values:

Unrepresentable_Domain: Allele scores (in base pairs) for each microsatellite locus.

Genetic Data From Nesting Steller’s Eiders (Polysticta stelleri), Near Utqiaġvik, Alaska, 1995-2016

Metadata: