Influenza A Virus Persistence Data from an Urban Wetland in Anchorage, Alaska, 2018-2019

Identification_Information:

Citation:

Citation_Information:

Originator: Reeves, A.B. (ORCID: 0000-0002-7526-0726)
Originator: Ramey, A.M. (ORCID: 0000-0002-3601-8400)
Originator: Koch, J. (0000-0001-7180-6982)
Originator: Poulson, R. L. (ORCID: 0000-0002-8087-3295)
Originator: Stallknecht, D.E.
Publication_Date: 20200114
Title:

Geospatial_Data_Presentation_Form: tabular digital data
Publication_Information:

Publication_Place: Anchorage, AK
Publisher: U.S. Geological Survey, Alaska Science Center

Other_Citation_Details:

Suggested Citation: Reeves, A.B., Ramey, A.M., Koch, J., Poulson, R.L., Stallknecht, D.E., 2020, Influenza A virus persistence data from an urban wetland in Anchorage, Alaska, 2018-2019: U.S. Geological Survey data release, https://doi.org/10.5066/P9B8XH6E

Online_Linkage: https://doi.org/10.5066/P9B8XH6E
Larger_Work_Citation:

Citation_Information:

Originator: U.S. Geological Survey, Alaska Science Center
Publication_Date: 2011
Title:

Assessment of wildlife health and disease in Alaska and adjacent regions

Geospatial_Data_Presentation_Form: website
Series_Information:

Series_Name: Alaska Science Portal
Issue_Identification: 321

Publication_Information:

Publication_Place: Anchorage, AK
Publisher: U.S. Geological Survey, Alaska Science Center

Other_Citation_Details:

This is a link to the broader USGS Alaska Science Center research project supported by these data. Users will find a description of the research project and links to associated reports, publications, and data products.

Online_Linkage: https://alaska.usgs.gov/portal/project.php?project_id=321

Description:

Abstract:

This dataset is three tables with details of samples and aliquots of waterfowl feces deposited in filtered surface water collected from an urban waterbody in Anchorage, Alaska in 2018-2019. Sample vials were submerged underwater in the same waterbody from which the samples were collected and the samples were tested for the presence and viability of influenza A virus. Temperature data and water chemistry measurements were also collected over time.

Purpose:

Data was collected to test the viability and detectability of viruses held under environmental conditions over time.

Time_Period_of_Content:

Time_Period_Information:

Range_of_Dates/Times:

Beginning_Date: 201809
Ending_Date: 201904

Currentness_Reference: ground condition

Status:

Progress: In work
Maintenance_and_Update_Frequency: As needed

Spatial_Domain:

Description_of_Geographic_Extent: Study area is Cuddy Family Midtown Park in Anchorage, Alaska.
Bounding_Coordinates:

West_Bounding_Coordinate: -149.886
East_Bounding_Coordinate: -149.875
North_Bounding_Coordinate: 61.187
South_Bounding_Coordinate: 61.180

Keywords:

Theme:

Theme_Keyword_Thesaurus: USGS Metadata Identifier
Theme_Keyword: USGS:ASC246

Theme:

Theme_Keyword_Thesaurus: ISO 19115 Topic Category
Theme_Keyword: biota

Theme:

Theme_Keyword_Thesaurus: NASA GCMD Earth Science Keywords Thesaurus
Theme_Keyword: Earth Science
Theme_Keyword: Biological Classification
Theme_Keyword: Animals/Vertebrates
Theme_Keyword: Birds
Theme_Keyword: Waterfowl
Theme_Keyword: Viruses

Theme:

Theme_Keyword_Thesaurus: USGS CSA Biocomplexity Thesaurus
Theme_Keyword: Waterfowl
Theme_Keyword: Aquatic animals
Theme_Keyword: Birds
Theme_Keyword: Viral Diseases
Theme_Keyword: Influenza

Theme:

Theme_Keyword_Thesaurus: USGS Thesaurus
Theme_Keyword: Health and disease
Theme_Keyword: Wildlife disease
Theme_Keyword: Avian influenza

Theme:

Theme_Keyword_Thesaurus: NIH MeSH Thesaurus
Theme_Keyword: Virus Diseases
Theme_Keyword: Influenza A virus

Place:

Place_Keyword_Thesaurus: USGS Geographic Names Information System
Place_Keyword: Alaska
Place_Keyword: Anchorage

Place:

Place_Keyword_Thesaurus: None
Place_Keyword: Cuddy Family Midtown Park

Taxonomy:

Keywords/Taxon:

Taxonomic_Keyword_Thesaurus: None
Taxonomic_Keywords: Animals
Taxonomic_Keywords: Vertebrates
Taxonomic_Keywords: Bird
Taxonomic_Keywords: Viruses
Taxonomic_Keywords: Influenza A virus

Taxonomic_System:

Classification_System/Authority:

Classification_System_Citation:

Citation_Information:

Originator: Integrated Taxonomic Information System
Publication_Date: Unknown
Title: Integrated Taxonomic Information System
Geospatial_Data_Presentation_Form: database
Publication_Information:

Publication_Place: online
Publisher: ITIS-North America

Other_Citation_Details:

Retrieved on July 16, 2019, from the Integrated Taxonomic Information System on-line database http://www.itis.gov

Online_Linkage: http://www.itis.gov

Classification_System/Authority:

Classification_System_Citation:

Citation_Information:

Originator: International Committee on Taxonomy of Viruses (ICTV)
Publication_Date: Unknown
Title: International Committee on Taxonomy of Viruses
Geospatial_Data_Presentation_Form: database
Publication_Information:

Publication_Place: online
Publisher: International Committee on Taxonomy of Viruses

Other_Citation_Details:

Taxonomic details for influenza A virus were retrieved July 16, 2019, from the International Committee on Taxonomy of Viruses online database https://talk.ictvonline.org/

Online_Linkage: https://talk.ictvonline.org/

Taxonomic_Procedures:

Influenza A viruses were identified by the successful amplification of an RNA influenza A matrix gene-target using real-time reverse transcriptase polymerase chain reaction (rRT-PCR).

Taxonomic_Classification:

Taxon_Rank_Name: Life
Taxon_Rank_Value: Life
Taxonomic_Classification:

Taxon_Rank_Name: Kingdom
Taxon_Rank_Value: Animalia
Applicable_Common_Name: Animal
Applicable_Common_Name: animaux
Applicable_Common_Name: animals
Taxonomic_Classification:

Taxon_Rank_Name: Subkingdom
Taxon_Rank_Value: Bilateria
Taxonomic_Classification:

Taxon_Rank_Name: Infrakingdom
Taxon_Rank_Value: Deuterostomia
Taxonomic_Classification:

Taxon_Rank_Name: Phylum
Taxon_Rank_Value: Chordata
Applicable_Common_Name: cordés
Applicable_Common_Name: cordado
Applicable_Common_Name: chordates
Taxonomic_Classification:

Taxon_Rank_Name: Subphylum
Taxon_Rank_Value: Vertebrata
Applicable_Common_Name: vertebrado
Applicable_Common_Name: vertébrés
Applicable_Common_Name: vertebrates
Taxonomic_Classification:

Taxon_Rank_Name: Infraphylum
Taxon_Rank_Value: Gnathostomata
Taxonomic_Classification:

Taxon_Rank_Name: Superclass
Taxon_Rank_Value: Tetrapoda
Taxonomic_Classification:

Taxon_Rank_Name: Class
Taxon_Rank_Value: Aves
Applicable_Common_Name: Birds
Applicable_Common_Name: oiseaux
Taxonomic_Classification:

Taxon_Rank_Name: Order
Taxon_Rank_Value: Anseriformes
Applicable_Common_Name: Waterfowl
Applicable_Common_Name: TSN 174982

Taxonomic_Classification:

Taxon_Rank_Name: Kingdom
Taxon_Rank_Value: viruses
Taxonomic_Classification:

Taxon_Rank_Name: Phylum
Taxon_Rank_Value: Negarnaviricota
Taxonomic_Classification:

Taxon_Rank_Name: Subphylum
Taxon_Rank_Value: Polyploviricotina
Taxonomic_Classification:

Taxon_Rank_Name: Class
Taxon_Rank_Value: Insthoviricetes
Taxonomic_Classification:

Taxon_Rank_Name: Order
Taxon_Rank_Value: Articulavirales
Taxonomic_Classification:

Taxon_Rank_Name: Family
Taxon_Rank_Value: Orthomyxoviridae
Taxonomic_Classification:

Taxon_Rank_Name: Genus
Taxon_Rank_Value: Alphainfluenzavirus
Taxonomic_Classification:

Taxon_Rank_Name: Species
Taxon_Rank_Value: Influenza A virus

Access_Constraints: None
Use_Constraints:

It is requested that the authors and the USGS Alaska Science Center be cited for any subsequent publications that reference this dataset.

Data_Set_Credit:

University of Georgia, Southeastern Cooperative Wildlife Disease Study, Athens, GA.

Cross_Reference:

Citation_Information:

Originator: Reeves, A.B.
Originator: Ramey, A.M.
Originator: Koch, J.
Originator: Poulson, R.L.
Originator: Stallknecht, D.E.
Publication_Date: 2020
Title:

Field-based method for assessing duration of infectivity for influenza A viruses in the environment

Geospatial_Data_Presentation_Form: journal article
Series_Information:

Series_Name: Journal of Virological Methods
Issue_Identification: 113818

Publication_Information:

Publication_Place: online
Publisher: Elsevier

Other_Citation_Details:

Reeves, A.B., Ramey, A.M., Koch, J., Poulson, R.L., Stallknecht, D.E. 2020. Field-based method for assessing duration of infectivity for influenza A viruses in the environment. Journal of Virological Methods 113818 doi:10.1016/j.jviromet.2020.113818

Online_Linkage: https://doi.org/10.1016/j.jviromet.2020.113818

Data_Quality_Information:

Attribute_Accuracy:

Attribute_Accuracy_Report:

Individual sample IDs, collection dates, sampling locations, and numbers of individual samples provided by collectors were cross-checked and verified to match the accompanying worksheet. GenBank accession numbers were cross-referenced to the appropriate individual samples as recorded.

Logical_Consistency_Report:

All values fall within expected ranges and data were checked for duplication and omission.

Completeness_Report:

Data set is considered complete for the information presented, as described in the abstract. Users are advised to read the rest of the metadata record carefully for additional details. Some sample aliquots were not tested and omitted from the spreadsheet if screening of initial sample aliquots provided no evidence detecting IAV.

Lineage:

Methodology:

Methodology_Type: Field
Methodology_Description:

Water was collected at Cuddy Family Midtown Park pond in Anchorage, Alaska and passed through a series of 1 micron and 0.22 micron filters.

Methodology:

Methodology_Type: Field
Methodology_Description:

Environmental Fecal Sampling: Swab samples for viral screening were collected by applying a polyester-tipped applicator to the feces. All swabs were placed in cryovials with chilled filtered surface water from Cuddy Family Midtown Park pond in Anchoage Alaska. Twenty aliquots were made from each sample and all but one aliquot from each sample were placed in mesh bags weighted with rocks and sealed in steel drums in approximately one meter of water.

Methodology:

Methodology_Type: Lab
Methodology_Description:

Environmental Fecal Sampling: All swab samples were initially screened at the University of Georgia using real-time reverse transcriptase polymerase chain reaction (rRT-PCR) targeting gene products for the influenza A virus matrix gene. All samples yielding Ct values < 45 for the IAV matrix gene were inoculated into specific pathogen-free embryonated chicken eggs for virus isolation. Amnio-allantoic fluids were collected after 120 hours and tested via hemagglutination assay. RNAs extracted from hemagglutination assay positive amnio-allantoic fluids were tested for the IAV matrix gene by rRT-PCR and resultant isolates were genomically sequenced at the Alaska Science Center using next-generation sequencing technology (Illumina platform, Illumina Inc., San Diego, CA).

Methodology:

Methodology_Type: Lab
Methodology_Description:

Measurements of dissolved oxygen (%) and pH in water samples were recorded using a portable electric handheld YSI (Yellow Springs, OH) EXO water quality meter. The device was calibrated for pH before use on the same day measurements were recorded.

Source_Information:

Source_Citation:

Citation_Information:

Originator: Spackman, E.
Originator: Senne, D.A.
Originator: Myers, T.J.
Originator: Bulaga, L.L.
Originator: Garber, L.P.
Originator: Perdue, M.L.
Originator: Lohman, K.
Originator: Daum, L.T.
Originator: Suarez, D.L.
Publication_Date: 2002
Title:

Development of a real-time reverse transcriptase PCR assay for type A influenza virus and the avian H5 and H7 hemagglutinin subtypes

Geospatial_Data_Presentation_Form: journal article
Series_Information:

Series_Name: Journal of Clinical Microbiology
Issue_Identification: 40(9):3256–32602

Publication_Information:

Publication_Place: online
Publisher: American Society for Microbiology

Other_Citation_Details:

Spackman, E., Senne, D.A., Myers, T.J., Bulaga, L.L., Garber, L.P., Perdue, M.L., Lohman, K., Daum, L.T., Suarez, D.L. 2002. Development of a real-time reverse transcriptase PCR assay for type A influenza virus and the avian H5 and H7 hemagglutinin subtypes. Journal of Clinical Microbiology 40(9):3256–32602. doi:10.1128/JCM.40.9.3256-3260.2002

Online_Linkage: https://doi.org/10.1128/JCM.40.9.3256-3260.2002

Type_of_Source_Media: paper
Source_Time_Period_of_Content:

Time_Period_Information:

Single_Date/Time:

Calendar_Date: 2002

Source_Currentness_Reference: observed

Source_Citation_Abbreviation: Spackman et al 2002
Source_Contribution: Methods used for detecting IAV in a sample using rRT-PCR

Source_Information:

Source_Citation:

Citation_Information:

Originator: Fouchier, R.A.M.
Originator: Bestebroer, T.M.
Originator: Herfst, S.
Originator: Van Der Kemp, L.
Originator: Rimmelzwaan, G.F.
Originator: Osterhaus, A.D.M.E..
Publication_Date: 2000
Title:

Detection of influenza A viruses from different species by PCR amplification of conserved sequences in the matrix gene. Journal of Clinical Microbiology.

Geospatial_Data_Presentation_Form: journal article
Series_Information:

Series_Name: Journal of Clinical Microbiology
Issue_Identification: 38(11):4096–4101

Publication_Information:

Publication_Place: online
Publisher: American Society for Microbiology

Other_Citation_Details:

Fouchier, R.A.M., Bestebroer, T.M., Herfst, S., Van Der Kemp, L., Rimmelzwaan, G.F., Osterhaus, A.D.M.E. 2000. Detection of influenza A viruses from different species by PCR amplification of conserved sequences in the matrix gene. Journal of Clinical Microbiology. Journal of Clinical Microbiology 38(11):4096–4101.

Type_of_Source_Media: paper
Source_Time_Period_of_Content:

Time_Period_Information:

Single_Date/Time:

Calendar_Date: 2000

Source_Currentness_Reference: observed

Source_Citation_Abbreviation: Fouchier et al 2000
Source_Contribution:

Methods used for detecting IAV in a sample using rRT-PCR and viral isolation methods

Process_Step:

Process_Description:

Nucleotide sequences from samples that were genomically sequenced were submitted to the National Center for Biotechnology Information (NCBI) GenBank website for public release http://www.ncbi.nlm.nih.gov/genbank/

Process_Date: 2019

Process_Step:

Process_Description:

Sample collection data were transcribed from hand-written field data collection sheets, and laboratory data collection spreadsheets created by staff at the University of Georgia, Southeastern Cooperative Wildlife Disease Study.

Process_Date: 2019

Spatial_Data_Organization_Information:

Indirect_Spatial_Reference:

Anchorage, Alaska, USA. Location names are representative of local geographic areas.

Spatial_Reference_Information:

Horizontal_Coordinate_System_Definition:

Geographic:

Latitude_Resolution: 0.001
Longitude_Resolution: 0.001
Geographic_Coordinate_Units: Decimal degrees

Geodetic_Model:

Horizontal_Datum_Name: World Geodetic System of 1984
Ellipsoid_Name: World Geodetic System of 1984
Semi-major_Axis: 6378137
Denominator_of_Flattening_Ratio: 298.257223563

Entity_and_Attribute_Information:

Detailed_Description:

Entity_Type:

Entity_Type_Label: avianFlu_iavResults_anchorage_reeves_2018_2019
Entity_Type_Definition:

Table presenting: 1) collection data for all environmental fecal samples from Cuddy Family Midtown Park in Anchorage, Alaska, 2018, 2) real-time reverse-transcriptase PCR and virus isolation results for influenza A viruses (IAV), and 3) genetic isolate sequence accession numbers. Presented in a Comma Separated Value (CSV) formatted table.

Entity_Type_Definition_Source: Author defined

Attribute:

Attribute_Label: Sample_ID
Attribute_Definition:

A unique identifier for each environmental fecal sample collected. Sample IDs are assigned by the person or persons collecting.

Attribute_Definition_Source: Author defined
Attribute_Domain_Values:

Unrepresentable_Domain:

Unique identifier assigned by the collector, composed of a two-letter location code and sequential sample numbers (e.g. S1, S2, S3...) and the targeted time the samples are retrieved for collecting (e.g. W0, W1, W2...).

Attribute:

Attribute_Label: Recovery_Date
Attribute_Definition:

Date the sample was recovered from the wetland. All sample aliquots were initially submerged in the wetland on October 24, 2018 except for samples with the Sample_ID suffix of 'W0' which were never submerged prior to IAV screening.

Attribute_Definition_Source: Author defined
Attribute_Domain_Values:

Range_Domain:

Range_Domain_Minimum: 10/31/2018
Range_Domain_Maximum: 03/05/2019
Attribute_Units_of_Measure: Date (MM/DD/YYYY)

Attribute_Domain_Values:

Enumerated_Domain:

Enumerated_Domain_Value: initial_sample
Enumerated_Domain_Value_Definition:

Not applicable, the sample was never submerged in the wetland, and was the initial sample screened for IAV.

Enumerated_Domain_Value_Definition_Source: Author defined

Attribute:

Attribute_Label: VI_Date
Attribute_Definition: The date viral isolation was attempted.
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:

Range_Domain:

Range_Domain_Minimum: 11/08/2018
Range_Domain_Maximum: 03/15/2019
Attribute_Units_of_Measure: Date (MM/DD/YYYY)

Attribute_Domain_Values:

Enumerated_Domain:

Enumerated_Domain_Value: NT
Enumerated_Domain_Value_Definition: Not tested, virus isolation was not attempted on this sample.
Enumerated_Domain_Value_Definition_Source: Author defined

Attribute:

Attribute_Label: rRT-PCR_Ct_Values
Attribute_Definition:

The number of cycles required for the fluorescent signal of a molecular probe for the influenza A matrix (MA) gene target to exceed background levels in a real-time reverse transcriptase polymerase chain reaction (rRT-PCR) applied to swab samples stored in viral transport media as described in Spackman et al 2002. The rRT-PCR is run for up to 45 cycles using reaction conditions described in the previous reference.

Attribute_Definition_Source: Author defined, with reference to: Spackman et al 2002.
Attribute_Domain_Values:

Range_Domain:

Range_Domain_Minimum: 21.15
Range_Domain_Maximum: 41.79

Attribute_Domain_Values:

Enumerated_Domain:

Enumerated_Domain_Value: No Ct
Enumerated_Domain_Value_Definition:

The sample was tested but did not exceed the background levels and therefore no Ct value was reported.

Enumerated_Domain_Value_Definition_Source: Author defined

Attribute_Domain_Values:

Enumerated_Domain:

Enumerated_Domain_Value: NT_inadequate_volume
Enumerated_Domain_Value_Definition:

sample was not tested due to inadequate volume of water/inoculum

Enumerated_Domain_Value_Definition_Source: Author defined

Attribute:

Attribute_Label: Final_VI_Result
Attribute_Definition:

Final result of viral isolation (VI) determined by the detection of the influenza A matrix gene target in RNA extracted from amnio-allantoic fluids using real-time reverse transcriptase polymerase chain reaction (rRT-PCR) methods described in Fouchier et al 2000. Only samples put into eggs have a reported result. Empty cells indicate eggs were not inoculated with a given sample.

Attribute_Definition_Source: Producer defined, with reference to: Fouchier et el 2000.
Attribute_Domain_Values:

Enumerated_Domain:

Enumerated_Domain_Value: POS
Enumerated_Domain_Value_Definition: Assay result positive
Enumerated_Domain_Value_Definition_Source: Author defined

Attribute_Domain_Values:

Enumerated_Domain:

Enumerated_Domain_Value: NEG
Enumerated_Domain_Value_Definition: Assay result negative
Enumerated_Domain_Value_Definition_Source: Author defined

Attribute:

Attribute_Label: GenBank_Accession_PB2
Attribute_Definition:

GenBank Accession numbers for influenza A virus gene segment 1, known as PB2. An accession number is a unique identifier given to a nucleotide sequence or other biological polymer sequence (DNA, protein) when it is submitted to NCBI GenBank database. "N/A" is the reported domain value when multiple sequences from an individual sample align to the same gene segment and could not be resolved. Genbank may be accessed at https://www.ncbi.nlm.nih.gov/genbank/. Empty cells indicate no influenza viruses were detected in and/or isolated from the sample or that a given isolate was not sequenced for this study.

Attribute_Definition_Source: Author defined
Attribute_Domain_Values:

Range_Domain:

Range_Domain_Minimum: MN477870
Range_Domain_Maximum: MN477886

Attribute:

Attribute_Label: GenBank_Accession_PB1
Attribute_Definition:

GenBank Accession numbers for influenza A virus gene segment 2, known as PB1. An accession number is a unique identifier given to a nucleotide sequence or other biological polymer sequence (DNA, protein) when it is submitted to NCBI GenBank database. "N/A" is the reported domain value when multiple sequences from an individual sample align to the same gene segment and could not be resolved. Genbank may be accessed at https://www.ncbi.nlm.nih.gov/genbank/. Empty cells indicate no influenza viruses were detected in and/or isolated from the sample or that a given isolate was not sequenced for this study.

Attribute_Definition_Source: Author defined
Attribute_Domain_Values:

Range_Domain:

Range_Domain_Minimum: MN477871
Range_Domain_Maximum: MN477887

Attribute:

Attribute_Label: GenBank_Accession_PA
Attribute_Definition:

GenBank Accession numbers for influenza A virus gene segment 3, known as PA. An accession number is a unique identifier given to a nucleotide sequence or other biological polymer sequence (DNA, protein) when it is submitted to NCBI GenBank database. "N/A" is the reported domain value when multiple sequences from an individual sample align to the same gene segment and could not be resolved. Genbank may be accessed at https://www.ncbi.nlm.nih.gov/genbank/. Empty cells indicate no influenza viruses were detected in and/or isolated from the sample or that a given isolate was not sequenced for this study.

Attribute_Definition_Source: Author defined
Attribute_Domain_Values:

Range_Domain:

Range_Domain_Minimum: MN477872
Range_Domain_Maximum: MN477888

Attribute:

Attribute_Label: GenBank_Accession_HA
Attribute_Definition:

GenBank Accession numbers for influenza A virus gene segment 4, known as HA. An accession number is a unique identifier given to a nucleotide sequence or other biological polymer sequence (DNA, protein) when it is submitted to NCBI GenBank database. "N/A" is the reported domain value when multiple sequences from an individual sample align to the same gene segment and could not be resolved. Genbank may be accessed at https://www.ncbi.nlm.nih.gov/genbank/. Empty cells indicate no influenza viruses were detected in and/or isolated from the sample or that a given isolate was not sequenced for this study.

Attribute_Definition_Source: Author defined
Attribute_Domain_Values:

Range_Domain:

Range_Domain_Minimum: MN477873
Range_Domain_Maximum: MN477889

Attribute:

Attribute_Label: GenBank_Accession_NP
Attribute_Definition:

GenBank Accession numbers for influenza A virus gene segment 5, known as NP. An accession number is a unique identifier given to a nucleotide sequence or other biological polymer sequence (DNA, protein) when it is submitted to NCBI GenBank database. "N/A" is the reported domain value when multiple sequences from an individual sample align to the same gene segment and could not be resolved. Genbank may be accessed at https://www.ncbi.nlm.nih.gov/genbank/. Empty cells indicate no influenza viruses were detected in and/or isolated from the sample or that a given isolate was not sequenced for this study.

Attribute_Definition_Source: Author defined
Attribute_Domain_Values:

Range_Domain:

Range_Domain_Minimum: MN477874
Range_Domain_Maximum: MN477890

Attribute:

Attribute_Label: GenBank_Accession_NA
Attribute_Definition:

GenBank Accession numbers for influenza A virus gene segment 6, known as NA. An accession number is a unique identifier given to a nucleotide sequence or other biological polymer sequence (DNA, protein) when it is submitted to NCBI GenBank database. "N/A" is the reported domain value when multiple sequences from an individual sample align to the same gene segment and could not be resolved. Genbank may be accessed at https://www.ncbi.nlm.nih.gov/genbank/. Empty cells indicate no influenza viruses were detected in and/or isolated from the sample or that a given isolate was not sequenced for this study.

Attribute_Definition_Source: Author defined
Attribute_Domain_Values:

Range_Domain:

Range_Domain_Minimum: MN477875
Range_Domain_Maximum: MN477891

Attribute:

Attribute_Label: GenBank_Accession_MA
Attribute_Definition:

GenBank Accession numbers for influenza A virus gene segment 7, known as MA. An accession number is a unique identifier given to a nucleotide sequence or other biological polymer sequence (DNA, protein) when it is submitted to NCBI GenBank database. "N/A" is the reported domain value when multiple sequences from an individual sample align to the same gene segment and could not be resolved. Genbank may be accessed at https://www.ncbi.nlm.nih.gov/genbank/. Empty cells indicate no influenza viruses were detected in and/or isolated from the sample or that a given isolate was not sequenced for this study.

Attribute_Definition_Source: Author defined
Attribute_Domain_Values:

Range_Domain:

Range_Domain_Minimum: MN477876
Range_Domain_Maximum: MN477892

Attribute:

Attribute_Label: GenBank_Accession_NS
Attribute_Definition:

GenBank Accession numbers for influenza A virus gene segment 8, known as NS. An accession number is a unique identifier given to a nucleotide sequence or other biological polymer sequence (DNA, protein) when it is submitted to NCBI GenBank database. "N/A" is the reported domain value when multiple sequences from an individual sample align to the same gene segment and could not be resolved. Genbank may be accessed at https://www.ncbi.nlm.nih.gov/genbank/. Empty cells indicate no influenza viruses were detected in and/or isolated from the sample or that a given isolate was not sequenced for this study.

Attribute_Definition_Source: Author defined
Attribute_Domain_Values:

Range_Domain:

Range_Domain_Minimum: MN477877
Range_Domain_Maximum: MN477893

Detailed_Description:

Entity_Type:

Entity_Type_Label: avianFlu_waterTemp_anchorage_reeves_2018_2019
Entity_Type_Definition:

Table presenting date, time and water temperature inside the container where samples are submerged in the pond at Cuddy Family Midtown Park in Anchorage, Alaska. Temperature was recorded at hourly intervals for the entire duration of the study. Presented in a Comma Separated Value (CSV) formatted table.

Entity_Type_Definition_Source: Author defined

Attribute:

Attribute_Label: Date_Time
Attribute_Definition:

The Date and time the temperature was recorded. Local Alaska time (GMT -0800) is displayed.

Attribute_Definition_Source: Author defined
Attribute_Domain_Values:

Range_Domain:

Range_Domain_Minimum: 2018-09-10 09:00:00
Range_Domain_Maximum: 2019-03-05 09:00:00
Attribute_Units_of_Measure: Date and time (YYYY-MM-DD HH:MM:SS)

Attribute:

Attribute_Label: Temp_C
Attribute_Definition: Temperature in degrees Celsius
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:

Range_Domain:

Range_Domain_Minimum: 0.95
Range_Domain_Maximum: 21.84
Attribute_Units_of_Measure: degrees Celsius

Detailed_Description:

Entity_Type:

Entity_Type_Label: avianFlu_waterChemisty_anchorage_reeves_2018_2019
Entity_Type_Definition:

Table presenting date, and water chemistry measurements for surface water from the pond at Cuddy Family Midtown Park in Anchorage, Alaska as well as the pooled filtered samples that were considered negative upon initial testing of samples. Presented in a Comma Separated Value (CSV) formatted table.

Entity_Type_Definition_Source: Author defined

Attribute:

Attribute_Label: Date
Attribute_Definition: The Date a sample was collected for testing
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:

Range_Domain:

Range_Domain_Minimum: 10/19/2018
Range_Domain_Maximum: 03/05/2019
Attribute_Units_of_Measure: Date (MM/DD/YYYY)

Attribute:

Attribute_Label: DO_percent_wetland_surface_water
Attribute_Definition:

Dissolved oxygen percentage measured from wetland (pond at Cuddy Family Midtown Park) surface water

Attribute_Definition_Source: Author defined
Attribute_Domain_Values:

Range_Domain:

Range_Domain_Minimum: 67.2
Range_Domain_Maximum: 113.6
Attribute_Units_of_Measure: percentage

Attribute:

Attribute_Label: DO_percent_pooled_water_from_sample_vials
Attribute_Definition:

Dissolved oxygen percentage measured from pooled and filtered samples for which previous aliquots were deemed negative for the detection of viable influenza A viruses.

Attribute_Definition_Source: Author defined
Attribute_Domain_Values:

Range_Domain:

Range_Domain_Minimum: 56.8
Range_Domain_Maximum: 89.6
Attribute_Units_of_Measure: percentage

Attribute_Domain_Values:

Enumerated_Domain:

Enumerated_Domain_Value: NT
Enumerated_Domain_Value_Definition:

The sample was not tested, samples were not available for this date.

Enumerated_Domain_Value_Definition_Source: Author defined

Attribute:

Attribute_Label: pH_wetland_surface_water
Attribute_Definition:

pH measured from wetland (pond at Cuddy Family Midtown Park) surface water

Attribute_Definition_Source: Author defined
Attribute_Domain_Values:

Range_Domain:

Range_Domain_Minimum: 6.55
Range_Domain_Maximum: 7.29
Attribute_Units_of_Measure: pH

Attribute:

Attribute_Label: pH_pooled_water_from_sample_vials
Attribute_Definition:

pH measured from pooled and filtered samples for which previous aliquots were deemed negative for the detection of viable influenza A viruses.

Attribute_Definition_Source: Author defined
Attribute_Domain_Values:

Range_Domain:

Range_Domain_Minimum: 7.12
Range_Domain_Maximum: 7.50
Attribute_Units_of_Measure: pH

Attribute_Domain_Values:

Enumerated_Domain:

Enumerated_Domain_Value: NT
Enumerated_Domain_Value_Definition:

The sample was not tested, samples were not available for this date.

Enumerated_Domain_Value_Definition_Source: Author defined

Influenza A Virus Persistence Data from an Urban Wetland in Anchorage, Alaska, 2018-2019

Metadata: