Metabarcoding of Feces of Pacific Walruses and Autosomal DNA Sequence Data of Marine Invertebrates, 2012-2015, Alaska.

Identification_Information:

Citation:

Citation_Information:

Originator: Sonsthagen, S.A. (ORCID: 0000-0001-6215-5874)
Originator: Cornman, R.S. (ORCID: 0000-0001-9511-2192)
Originator: Fischbach, A.S. (ORCID: 0000-0002-6555-865X)
Originator: Jay, C.V. (ORCID: 0000-0002-9559-2189)
Originator: Grebmeier, J.M. (ORCID: 0000-0001-7624-3568)
Originator: Talbot, S.L.(ORCID: 0000-0002-3312-7214)
Publication_Date: 20200721
Title:

Geospatial_Data_Presentation_Form: tabular digital data
Publication_Information:

Publication_Place: Anchorage, Alaska
Publisher: U.S. Geological Survey, Alaska Science Center

Other_Citation_Details:

Suggested Citation: Sonsthagen, S.A., Jay, C.V., Cornman, R.S., Fischbach, A.S., Grebmeier, J.M., Talbot, S.L., 2020, Metabarcoding of feces of Pacific walruses and autosomal DNA sequence data of marine invertebrates, 2012-2015, Alaska: U.S. Geological Survey data release, https://doi.org/10.5066/P9WWMPLT

Online_Linkage: https://doi.org/10.5066/P9WWMPLT
Larger_Work_Citation:

Citation_Information:

Originator: U.S. Geological Survey, Alaska Science Center
Publication_Date: 2000
Title: Genomics of Wildlife
Geospatial_Data_Presentation_Form: website
Series_Information:

Series_Name: Alaska Science Portal
Issue_Identification: 416

Publication_Information:

Publication_Place: Anchorage, Alaska
Publisher: U.S. Geological Survey, Alaska Science Center

Other_Citation_Details:

This is a link to the broader USGS Alaska Science Center research project supported by these data. Users will find a description of the research project and links to associated reports, publications, and data products.

Online_Linkage: https://alaska.usgs.gov/portal/project.php?project_id=416

Description:

Abstract:

This data set describes nucleotide sequence data derived from 18S ribosomal DNA amplified in two fragments. A total of 87 feces from Pacific walrus and 57 marine invertebrates were examined for this study. Samples were collected from the Bering Sea and Chukchi Sea, Alaska. Samples used in the study originated from feces or muscle samples collected in the field from ice floes or benthic van Veen grab samples.

Purpose: To infer the summer diets of Pacific walrus.

Time_Period_of_Content:

Time_Period_Information:

Range_of_Dates/Times:

Beginning_Date: 2012
Ending_Date: 2018

Currentness_Reference: Lab

Status:

Progress: Complete
Maintenance_and_Update_Frequency: None planned

Spatial_Domain:

Description_of_Geographic_Extent: Bering Sea north to Chukchi Sea, Alaska, USA.
Bounding_Coordinates:

West_Bounding_Coordinate: -175.043
East_Bounding_Coordinate: -157.908
North_Bounding_Coordinate: 71.943
South_Bounding_Coordinate: 62.041

Keywords:

Theme:

Theme_Keyword_Thesaurus: USGS Metadata Identifier
Theme_Keyword: USGS:ASC283

Theme:

Theme_Keyword_Thesaurus: ISO 19115 Topic Category
Theme_Keyword: Biota
Theme_Keyword: Environment

Theme:

Theme_Keyword_Thesaurus: NASA GCMD Earth Science Thesaurus
Theme_Keyword: Animals/Vertebrates
Theme_Keyword: Mammals
Theme_Keyword: Animals/Invertebrates
Theme_Keyword: Bivalves
Theme_Keyword: Clams
Theme_Keyword: Benthic habitat

Theme:

Theme_Keyword_Thesaurus: USGS CSA Biocomplexity Thesaurus
Theme_Keyword: Marine mammals
Theme_Keyword: Foraging behavior

Theme:

Theme_Keyword_Thesaurus: USGS Thesaurus
Theme_Keyword: Wildlife
Theme_Keyword: Macroinvertebrates
Theme_Keyword: Marine ecosystems
Theme_Keyword: Benthos
Theme_Keyword: Genetics
Theme_Keyword: Polymerase chain reaction
Theme_Keyword: DNA sequencing
Theme_Keyword: Environmental DNA

Theme:

Theme_Keyword_Thesaurus: MeSH-National Library of Medicine
Theme_Keyword: Genomics

Theme:

Theme_Keyword_Thesaurus: None
Theme_Keyword: eDNA
Theme_Keyword: Pacific Walrus
Theme_Keyword: Odobenus rosmarus divergens

Place:

Place_Keyword_Thesaurus: USGS Geographic Names Information System
Place_Keyword: Bering Sea
Place_Keyword: Chukchi Sea

Taxonomy:

Keywords/Taxon:

Taxonomic_Keyword_Thesaurus: None
Taxonomic_Keywords: Animals
Taxonomic_Keywords: Marine mammals
Taxonomic_Keywords: Marine invertebrates

Taxonomic_System:

Classification_System/Authority:

Classification_System_Citation:

Citation_Information:

Originator: ITIS Integrated Taxonomic Information System
Publication_Date: Unknown
Title: ITIS Integrated Taxonomic Information System
Geospatial_Data_Presentation_Form: database
Series_Information:

Series_Name: Taxonomic Serial Number
Issue_Identification:

TSN 79118, 69459, 78807, 64358, 568832, 57490, 57413, 914198, 155471, 155153, 89787, 158854, 180639, 622045, AphiaID 1320351

Publication_Information:

Publication_Place: online
Publisher: ITIS-North America

Other_Citation_Details:

Taxonomic details were retrieved May 27, 2020, from the Integrated Taxonomic Information System online database https://www.itis.gov

Online_Linkage: https://www.itis.gov

Taxonomic_Procedures:

Field collected marine invertebrates were identified by skilled observers based on morphological characteristics. Operational Taxon Units (OTU) were identified genetically by comparison of DNA sequences to sequences available on NCBI Genbank using the Basic Local Alignment Search Tool (BLAST).

Taxonomic_Completeness:

Provided here is the classification of marine invertebrates to the rank of class. Further classification of individual samples and OTUs to the lowest possible taxonomic rank are provided in the table "walrus_dietMetabarcoding_taxa_alaska_sonsthagen_2012_2018.csv" included with this data package.

Taxonomic_Classification:

Taxon_Rank_Name: Kingdom
Taxon_Rank_Value: Animalia
Taxonomic_Classification:

Taxon_Rank_Name: Subkingdom
Taxon_Rank_Value: Bilateria
Taxonomic_Classification:

Taxon_Rank_Name: Infrakingdom
Taxon_Rank_Value: Protostomia
Taxonomic_Classification:

Taxon_Rank_Name: Superphylum
Taxon_Rank_Value: Lophozoa
Taxonomic_Classification:

Taxon_Rank_Name: Phylum
Taxon_Rank_Value: Mollusca
Taxonomic_Classification:

Taxon_Rank_Name: Class
Taxon_Rank_Value: Bivalvia
Applicable_Common_Name: TSN: 79118

Taxonomic_Classification:

Taxon_Rank_Name: Class
Taxon_Rank_Value: Gastropoda
Applicable_Common_Name: TSN: 69459

Taxonomic_Classification:

Taxon_Rank_Name: Class
Taxon_Rank_Value: Polyplacophora
Applicable_Common_Name: TSN: 78807

Taxonomic_Classification:

Taxon_Rank_Name: Phylum
Taxon_Rank_Value: Annelida
Taxonomic_Classification:

Taxon_Rank_Name: Class
Taxon_Rank_Value: Polychaeta
Applicable_Common_Name: TSN: 64358

Taxonomic_Classification:

Taxon_Rank_Name: Class
Taxon_Rank_Value: Clitellata
Applicable_Common_Name: TSN: 568832

Taxonomic_Classification:

Taxon_Rank_Name: Phylum
Taxon_Rank_Value: Nemertea
Taxonomic_Classification:

Taxon_Rank_Name: Class
Taxon_Rank_Value: Enopla
Applicable_Common_Name: TSN: 57490

Taxonomic_Classification:

Taxon_Rank_Name: Class
Taxon_Rank_Value: Anopla
Applicable_Common_Name: TSN: 57413

Taxonomic_Classification:

Taxon_Rank_Name: Class
Taxon_Rank_Value: Pilidiophora
Applicable_Common_Name: AphiaID: 1320351

Taxonomic_Classification:

Taxon_Rank_Name: Phylum
Taxon_Rank_Value: Brachiopoda
Taxonomic_Classification:

Taxon_Rank_Name: Subphylum
Taxon_Rank_Value: Rhynchonelliformea
Taxonomic_Classification:

Taxon_Rank_Name: Class
Taxon_Rank_Value: Rhynchonellata
Applicable_Common_Name: TSN: 914198

Taxonomic_Classification:

Taxon_Rank_Name: Phylum
Taxon_Rank_Value: Bryozoa
Taxonomic_Classification:

Taxon_Rank_Name: Class
Taxon_Rank_Value: Gymnolaemata
Applicable_Common_Name: TSN: 155471

Taxonomic_Classification:

Taxon_Rank_Name: Superphylum
Taxon_Rank_Value: Ecdysozoa
Taxonomic_Classification:

Taxon_Rank_Name: Phylum
Taxon_Rank_Value: Priapulida
Applicable_Common_Name: TSN: 155153

Taxonomic_Classification:

Taxon_Rank_Name: Phylum
Taxon_Rank_Value: Arthropoda
Taxonomic_Classification:

Taxon_Rank_Name: Subphylum
Taxon_Rank_Value: Crustacea
Taxonomic_Classification:

Taxon_Rank_Name: Class
Taxon_Rank_Value: Malacostraca
Applicable_Common_Name: TSN: 89787

Taxonomic_Classification:

Taxon_Rank_Name: Infrakingdom
Taxon_Rank_Value: Deuterostomia
Taxonomic_Classification:

Taxon_Rank_Name: Phylum
Taxon_Rank_Value: Chordata
Taxonomic_Classification:

Taxon_Rank_Name: Subphylum
Taxon_Rank_Value: Urochordata
Taxonomic_Classification:

Taxon_Rank_Name: Class
Taxon_Rank_Value: Ascidiacea
Applicable_Common_Name: TSN: 158854

Taxonomic_Classification:

Taxon_Rank_Name: Subphylum
Taxon_Rank_Value: Vertebrata
Taxonomic_Classification:

Taxon_Rank_Name: Infraphylum
Taxon_Rank_Value: Gnathostomata
Taxonomic_Classification:

Taxon_Rank_Name: Superclass
Taxon_Rank_Value: Tetrapoda
Taxonomic_Classification:

Taxon_Rank_Name: Class
Taxon_Rank_Value: Mammalia
Taxonomic_Classification:

Taxon_Rank_Name: Subclass
Taxon_Rank_Value: Theria
Taxonomic_Classification:

Taxon_Rank_Name: Infraclass
Taxon_Rank_Value: Eutheria
Taxonomic_Classification:

Taxon_Rank_Name: Order
Taxon_Rank_Value: Carnivora
Taxonomic_Classification:

Taxon_Rank_Name: Suborder
Taxon_Rank_Value: Caniformia
Taxonomic_Classification:

Taxon_Rank_Name: Family
Taxon_Rank_Value: Odobenidae
Taxonomic_Classification:

Taxon_Rank_Name: Genus
Taxon_Rank_Value: Odobenus
Taxonomic_Classification:

Taxon_Rank_Name: Species
Taxon_Rank_Value: Odobenus rosmarus
Applicable_Common_Name: Walrus
Applicable_Common_Name: TSN: 180639
Taxonomic_Classification:

Taxon_Rank_Name: Subspecies
Taxon_Rank_Value: Odobenus rosmarus divergens
Applicable_Common_Name: Pacific Walrus
Applicable_Common_Name: TSN: 622045

Taxonomic_Classification:

Taxon_Rank_Name: Phylum
Taxon_Rank_Value: Echinodermata
Taxonomic_Classification:

Taxon_Rank_Name: Subphylum
Taxon_Rank_Value: Echinozoa
Taxonomic_Classification:

Taxon_Rank_Name: Class
Taxon_Rank_Value: Holothuroidea
Applicable_Common_Name: TSN: 158140

Taxonomic_Classification:

Taxon_Rank_Name: Class
Taxon_Rank_Value: Echinoidea
Applicable_Common_Name: TSN: 157821

Taxonomic_Classification:

Taxon_Rank_Name: Subphylum
Taxon_Rank_Value: Asterozoa
Taxonomic_Classification:

Taxon_Rank_Name: Class
Taxon_Rank_Value: Asteroidea
Applicable_Common_Name: TSN: 156862

Taxonomic_Classification:

Taxon_Rank_Name: Class
Taxon_Rank_Value: Ophiuroidea
Applicable_Common_Name: TSN: 157325

Taxonomic_Classification:

Taxon_Rank_Name: Subkingdom
Taxon_Rank_Value: Radiata
Taxonomic_Classification:

Taxon_Rank_Name: Phylum
Taxon_Rank_Value: Cnidaria
Taxonomic_Classification:

Taxon_Rank_Name: Subphylum
Taxon_Rank_Value: Anthozoa
Applicable_Common_Name: TSN: 914163

Taxonomic_Classification:

Taxon_Rank_Name: Subphylum
Taxon_Rank_Value: Medusozoa
Taxonomic_Classification:

Taxon_Rank_Name: Class
Taxon_Rank_Value: Scyphozoa
Applicable_Common_Name: TSN: 51483

Taxonomic_Classification:

Taxon_Rank_Name: Class
Taxon_Rank_Value: Hydrozoa
Applicable_Common_Name: TSN: 48739

Taxonomic_Classification:

Taxon_Rank_Name: Phylum
Taxon_Rank_Value: Porifera
Taxonomic_Classification:

Taxon_Rank_Name: Class
Taxon_Rank_Value: Demospongiae
Applicable_Common_Name: TSN: 47528

Taxonomic_Classification:

Taxon_Rank_Name: Class
Taxon_Rank_Value: Calcarea
Applicable_Common_Name: TSN: 46862

Access_Constraints: None
Use_Constraints:

It is requested that the authors and the USGS Alaska Science Center be cited for any subsequent publications that reference this dataset.

Point_of_Contact:

Contact_Information:

Contact_Organization_Primary:

Contact_Organization: U.S. Geological Survey, Alaska Science Center

Contact_Address:

Address_Type: Mailing and Physical
Address: 4210 University Drive
City: Anchorage
State_or_Province: Alaska
Postal_Code: 99508
Country: USA

Contact_Voice_Telephone: 907-786-7000
Contact_Electronic_Mail_Address: ascweb@usgs.gov

Cross_Reference:

Citation_Information:

Originator: Sonsthagen, S.A. (ORCID: 0000-0001-6215-5874)
Originator: Jay, C.V. (ORCID: 0000-0002-9559-2189)
Originator: Cornman, R.S. (ORCID: 0000-0001-9511-2192)
Originator: Fischbach, A.S. (ORCID: 0000-0002-6555-865X)
Originator: Grebmeier, J.M. (ORCID: 0000-0001-7624-3568)
Originator: Talbot, S.L. (ORCID: 0000-0002-3312-721)
Publication_Date: 2020
Title:

DNA metabarcoding of feces to infer summer diet of Pacific walruses

Geospatial_Data_Presentation_Form: journal article
Series_Information:

Series_Name: Marine Mammal Science
Issue_Identification: 36

Publication_Information:

Publication_Place: online
Publisher: John Wiley and Sons

Other_Citation_Details:

Sonsthagen, S.A., Jay, C.V., Cornman, R.S., Fischbach, A.S., Grebmeier, J.M., Talbot, S.L., 2020. DNA metabarcoding of feces to infer summer diet of Pacific walruses. Marine Mammal Science 36 doi:10.1111/mms.12717

Online_Linkage: https://doi.org/10.1111/mms.12717

Data_Quality_Information:

Attribute_Accuracy:

Attribute_Accuracy_Report:

Identifying information about samples were cross-checked with original field data. Polymerase chain reaction (PCR) amplification followed protocols described in Sonsthagen et al 2020. All laboratory techniques were performed using sterile procedures.

Logical_Consistency_Report:

Primers and adapters were trimmed using the default scoring algorithm in CLC Genomics v. 9.0 (Qiagen Inc.). Bases were trimmed on a maximum error probability of 0.01, with at most two ambiguous bases allowed and a minimum trimmed length of 50 bp. Orphaned reads were discarded. Trimmed read pairs were merged with the default merge parameters; merged reads shorter than 100 bp were discarded. See Sonsthagen et al. 2020 for additional information.

Completeness_Report: Blank cells are described for each attribute.
Lineage:

Methodology:

Methodology_Type: Field
Methodology_Description:

Fecal samples were collected from ice floes, stored in plastic bags and frozen. Additional details on field techniques are available in Sonsthagen et al (2020)

Methodology:

Methodology_Type: Lab
Methodology_Description:

DNA was extracted using a QIAamp DNA Stool Kit (Qiagen, Valencia, CA) or Qiagen Dneasy Blood and Tissue extraction kit (QIAGEN, Valencia, CA) following manufacturer’s protocol.

Methodology:

Methodology_Type: Lab
Methodology_Description:

Locus-specific primers were tailed with Illumina adaptors (P5 and P7) and indices following Nextera chemistry (Illumina, San Diego, CA). Polymerase chain reaction (PCR) amplifications were carried out in triplicate. Pooled PCR products were electrophoresed on a 1.5% agarose gel and gel purified using a MinElute Gel Extraction Kit (Qiagen) to ensure no primer dimers were present in the final library. Additional details on laboratory techniques are available in Sonsthagen et al (2020).

Source_Information:

Source_Citation:

Citation_Information:

Originator: National Center for Biotechnology Information (NCBI)
Publication_Date: Unknown
Title: BioProject
Geospatial_Data_Presentation_Form: database
Online_Linkage: https://www.ncbi.nlm.nih.gov/bioproject/

Type_of_Source_Media: digital database file
Source_Time_Period_of_Content:

Time_Period_Information:

Single_Date/Time:

Calendar_Date: 2020

Source_Currentness_Reference: observed

Source_Citation_Abbreviation: NCBI BioProject
Source_Contribution:

Information regarding sampling, biological source material, and sequences obtained in this study are publicly accessible in the NCBI BioProject database (BioProject accession number PRJNA635240).

Source_Information:

Source_Citation:

Citation_Information:

Originator: National Center for Biotechnology Information (NCBI)
Publication_Date: Unknown
Title: BioSample
Geospatial_Data_Presentation_Form: database
Online_Linkage: https://www.ncbi.nlm.nih.gov/biosample/

Type_of_Source_Media: digital database file
Source_Time_Period_of_Content:

Time_Period_Information:

Single_Date/Time:

Calendar_Date: 2020

Source_Currentness_Reference: observed

Source_Citation_Abbreviation: NCBI BioSample
Source_Contribution:

Information regarding sampling and the biological source material used in this study are publicly accessible in the NCBI BioSample database (accession numbers provided in this data package: "walrus_metabarcodingSampleDetails_alaska_sonsthagen_2012_2018.csv").

Source_Information:

Source_Citation:

Citation_Information:

Originator: National Center for Biotechnology Information (NCBI)
Publication_Date: Unknown
Title: Sequence Read Archive (SRA)
Geospatial_Data_Presentation_Form: database
Online_Linkage: https://www.ncbi.nlm.nih.gov/sra

Type_of_Source_Media: digital database file
Source_Time_Period_of_Content:

Time_Period_Information:

Single_Date/Time:

Calendar_Date: 2020

Source_Currentness_Reference: observed

Source_Citation_Abbreviation: NCBI SRA
Source_Contribution:

Raw Illumina reads are publicly accessible in the NCBI SRA database

Source_Information:

Source_Citation:

Citation_Information:

Originator: National Center for Biotechnology Information (NCBI)
Publication_Date: Unknown
Title: GenBank
Geospatial_Data_Presentation_Form: database
Online_Linkage: https://www.ncbi.nlm.nih.gov/genbank/

Type_of_Source_Media: digital database file
Source_Time_Period_of_Content:

Time_Period_Information:

Single_Date/Time:

Calendar_Date: 2020

Source_Currentness_Reference: observed

Source_Citation_Abbreviation: NCBI GenBank
Source_Contribution:

Sequence information is publicly accessible in the NCBI Genbank database (accession numbers provided in this data package: "walrus_metabarcodingSampleDetails_alaska_sonsthagen_2012_2018.csv")

Source_Information:

Source_Citation:

Citation_Information:

Originator: National Center for Biotechnology Information (NCBI)
Publication_Date: Unknown
Title: Basic Local Alignment Search Tool (BLAST)
Geospatial_Data_Presentation_Form: application/service
Online_Linkage: https://blast.ncbi.nlm.nih.gov/Blast.cgi

Type_of_Source_Media: digital database file
Source_Time_Period_of_Content:

Time_Period_Information:

Single_Date/Time:

Calendar_Date: 2020

Source_Currentness_Reference: observed

Source_Citation_Abbreviation: NCBI BLAST
Source_Contribution:

Nucleotide sequences obtained from fecal samples were queried against known sequences in NCBI GenBank using BLAST to identify Operational Taxon Units (OTU).

Source_Information:

Source_Citation:

Citation_Information:

Originator: Sonsthagen, S.A.
Originator: Jay, C.V.
Originator: Cornman, R.S.
Originator: Fischbach, A.S.
Originator: Grebmeier, J.M.
Originator: Talbot, S.L.
Publication_Date: 2020
Title:

DNA metabarcoding of feces to infer summer diet of Pacific walruses

Geospatial_Data_Presentation_Form: journal article
Series_Information:

Series_Name: Marine Mammal Science
Issue_Identification: 36

Publication_Information:

Publication_Place: online
Publisher: John Wiley and Sons

Other_Citation_Details:

Online_Linkage: https://doi.org/10.1111/mms.12717

Type_of_Source_Media: publication
Source_Time_Period_of_Content:

Time_Period_Information:

Single_Date/Time:

Calendar_Date: 2020

Source_Currentness_Reference: publication date

Source_Citation_Abbreviation: Sonsthagen et al 2020
Source_Contribution:

Describes DNA amplification, preparation of Illumina libraries, and sequencing on MiSeq benchtop sequencer.

Process_Step:

Process_Description:

Nucleotide sequences obtained from fecal samples were queried against known sequences in NCBI GenBank using BLAST to identify Operational Taxon Units (OTU).

Process_Date: Unknown

Process_Step:

Process_Description:

Raw Illumina reads were submitted to the NCBI Short Read Archive and 18S nucleotide sequences were submitted to the NCBI GenBank database.

Process_Date: Unknown

Spatial_Data_Organization_Information:

Indirect_Spatial_Reference:

Sea names are listed to describe generalized field sampling locations.

Direct_Spatial_Reference_Method: Point

Spatial_Reference_Information:

Horizontal_Coordinate_System_Definition:

Geographic:

Latitude_Resolution: 0.001
Longitude_Resolution: 0.001
Geographic_Coordinate_Units: Decimal degrees

Geodetic_Model:

Horizontal_Datum_Name: World Geodetic System of 1984
Ellipsoid_Name: World Geodetic System of 1984
Semi-major_Axis: 6378137
Denominator_of_Flattening_Ratio: 298.257223563

Entity_and_Attribute_Information:

Detailed_Description:

Entity_Type:

Entity_Type_Label:

walrus_dietMetabarcoding_sampleDetails_alaska_sonsthagen_2012_2018

Entity_Type_Definition:

Table with sample identifying information, collection data, and NCBI GenBank accessions for nucleotide sequence data and NCBI BioSample accession numbers for high throughput sequence data. Presented in a Comma Separated Value (CSV) formatted table.

Entity_Type_Definition_Source: Author defined

Attribute:

Attribute_Label: Sample_ID
Attribute_Definition:

USGS Alaska Science Center, Molecular Ecology Laboratory (MEL) alphanumeric label that uniquely identifies each sample.

Attribute_Definition_Source: Author defined
Attribute_Domain_Values:

Unrepresentable_Domain:

Unique identifier; an alphanumeric code for each individual sample in this dataset.

Attribute:

Attribute_Label: Species_ID
Attribute_Definition:

This indicates the taxonomic identification of a sample based upon morphology.

Attribute_Definition_Source: Author defined
Attribute_Domain_Values:

Unrepresentable_Domain: Taxonomic identification of a sample.

Attribute:

Attribute_Label: Sample_Type
Attribute_Definition: Type of tissue from which sample DNA as collected
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:

Enumerated_Domain:

Enumerated_Domain_Value: feces
Enumerated_Domain_Value_Definition: Walrus feces collected from ice floe.
Enumerated_Domain_Value_Definition_Source: Author defined

Attribute_Domain_Values:

Enumerated_Domain:

Enumerated_Domain_Value: muscle
Enumerated_Domain_Value_Definition: Muscle sample taken from collected marine invertebrate.
Enumerated_Domain_Value_Definition_Source: Author defined

Attribute:

Attribute_Label: Sample_location
Attribute_Definition: Place name of sample collection sites.
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:

Enumerated_Domain:

Enumerated_Domain_Value: USA: Alaska, Chukchi Sea
Enumerated_Domain_Value_Definition: Samples collected from the Chukchi Sea, Alaska.
Enumerated_Domain_Value_Definition_Source: Geographic Names Information System (GNIS)

Attribute_Domain_Values:

Enumerated_Domain:

Enumerated_Domain_Value: USA: Alaska, Bering Sea
Enumerated_Domain_Value_Definition: Samples collected from the Bering Sea, Alaska.
Enumerated_Domain_Value_Definition_Source: Geographic Names Information System (GNIS)

Attribute:

Attribute_Label: Collection_Date
Attribute_Definition: Date that sample was collected in the field.
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:

Range_Domain:

Range_Domain_Minimum: 2012-07-12
Range_Domain_Maximum: 2015-07-19
Attribute_Units_of_Measure: Date (YYYY-MM-DD)

Attribute:

Attribute_Label: Latitude
Attribute_Definition: The latitude of the field sample collection.
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:

Range_Domain:

Range_Domain_Minimum: 62.041
Range_Domain_Maximum: 71.943
Attribute_Units_of_Measure: Decimal degrees (WGS84)

Attribute:

Attribute_Label: Longitude
Attribute_Definition: The longitude of the field sample collection.
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:

Range_Domain:

Range_Domain_Minimum: -157.908
Range_Domain_Maximum: -175.043
Attribute_Units_of_Measure: Decimal degrees (WGS84)

Attribute:

Attribute_Label: BioSample_accession
Attribute_Definition:

BioSample Accession Number. A unique identifier (assigned by the National Center for Biotechnology Information; NCBI) of the biological source material used in this study. This number may be used to retrieve detailed sample and experiment information from the NCBI BioSample database ( https://www.ncbi.nlm.nih.gov/biosample ). Blank cells indicate that source data were not provided for this sample.

Attribute_Definition_Source: NCBI BioSample
Attribute_Domain_Values:

Unrepresentable_Domain:

Unique identifier: an alphanumeric accession number available in the NCBI BioSample database.

Attribute:

Attribute_Label: 18S735_accession
Attribute_Definition:

GenBank Accession Number. A unique identifier (assigned by the National Center for Biotechnology Information; NCBI) of the 18S ribosomal nucleotide sequence, sequenced from the primer pair 18S735:18S1679 (Sonsthagen et al. 2020). This number may be used to retrieve sequence information from the NCBI GenBank database ( https://www.ncbi.nlm.nih.gov/genbank ). Blank cells indicate that sequence data from this locus were not collected for this sample.

Attribute_Definition_Source: NCBI GenBank
Attribute_Domain_Values:

Unrepresentable_Domain:

Unique identifier: an alphanumeric accession number available in the NCBI GenBank database

Attribute:

Attribute_Label: 18S1837_accession
Attribute_Definition:

NCBI GenBank Accession Number. A unique identifier (assigned by the National Center for Biotechnology Information; NCBI) of the 18S ribosomal nucleotide sequence, sequenced from the primer pair 18S1837:18S2396 (Sonsthagen et al. 2020). This number may be used to retrieve sequence information from the NCBI GenBank database ( https://www.ncbi.nlm.nih.gov/genbank ). Blank cells indicate that sequence data from this locus were not collected for this sample.

Attribute_Definition_Source: NCBI GenBank
Attribute_Domain_Values:

Unrepresentable_Domain:

Unique identifier: an alphanumeric accession number available in the NCBI GenBank database

Detailed_Description:

Entity_Type:

Entity_Type_Label:

walrus_dietMetabarcoding_OTUassignment_alaska_sonsthagen_2012_2018

Entity_Type_Definition:

Table with the number of reads assigned to Operational Taxon Units (OTU) listed by walrus fecal sample. Counts are summed across OTUs with the same taxonomic rank assignment, but are not aggregated at successive ranks. That is, genus counts do not include species counts, family counts do not include genus counts etc. Blank cells indicate that an OTU could not be assigned to a particular taxonomic rank. Presented in a Comma Separated Value (CSV) formatted table.

Entity_Type_Definition_Source: Author defined

Attribute:

Attribute_Label: Class
Attribute_Definition: Class of the OTU
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:

Unrepresentable_Domain: Class of the OTU

Attribute:

Attribute_Label: Order
Attribute_Definition: Order of the OTU
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:

Unrepresentable_Domain: Order of the OTU

Attribute:

Attribute_Label: Family
Attribute_Definition: Family of the OTU
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:

Unrepresentable_Domain: Family of the OTU

Attribute:

Attribute_Label: Genus
Attribute_Definition: Genus of the OTU
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:

Unrepresentable_Domain: Genus of the OTU

Attribute:

Attribute_Label: Species
Attribute_Definition: Species of the OTU
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:

Unrepresentable_Domain: Species of the OTU

Attribute:

Attribute_Label: NM12-S01 through W14-S012
Attribute_Definition:

Each attribute represents one walrus fecal sample. Attribute labels correspond to "Sample_ID" value in the table "walrus_dietMetabarcoding_sampleDetails_alaska_sonsthagen_2012_2018.csv" included with this data package. Each record provides the number of reads assigned to the OTU for each walrus fecal sample.

Attribute_Definition_Source: Author defined
Attribute_Domain_Values:

Unrepresentable_Domain: Number of reads assigned to the OTU

Detailed_Description:

Entity_Type:

Entity_Type_Label: walrus_dietMetabarcoding_taxa_alaska_sonsthagen_2012_2018
Entity_Type_Definition:

Table with the taxonomic details of marine invertebrates from field collected samples and genetically determined Operational Taxon Units (OTU). Blank cells indicate that a sample or OTU could not be assigned to a particular taxonomic rank. Presented in a Comma Separated Value (CSV) formatted table.

Entity_Type_Definition_Source: Author defined

Attribute:

Attribute_Label: Class
Attribute_Definition: Class of sample or OTU
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:

Unrepresentable_Domain: Class of sample or OTU

Attribute:

Attribute_Label: Order
Attribute_Definition: Order of sample or OTU
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:

Unrepresentable_Domain: Order of sample or OTU

Attribute:

Attribute_Label: Family
Attribute_Definition: Family of sample or OTU
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:

Unrepresentable_Domain: Family of sample or OTU

Attribute:

Attribute_Label: Genus
Attribute_Definition: Genus of sample or OTU
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:

Unrepresentable_Domain: Genus of sample or OTU

Attribute:

Attribute_Label: Species
Attribute_Definition: Species of sample or OTU
Attribute_Definition_Source: Author defined
Attribute_Domain_Values:

Unrepresentable_Domain: Species of sample or OTU

Attribute:

Attribute_Label: Sample_type
Attribute_Definition:

Indicates whether the taxonomy describes a marine invertebrate collected in the field or a genetically determined Operational Taxon Unit (OTU)

Attribute_Definition_Source: Author defined
Attribute_Domain_Values:

Enumerated_Domain:

Enumerated_Domain_Value: Field Collection
Enumerated_Domain_Value_Definition: Taxonomy describes a field collected sample
Enumerated_Domain_Value_Definition_Source: Author defined

Attribute_Domain_Values:

Enumerated_Domain:

Enumerated_Domain_Value: OTU
Enumerated_Domain_Value_Definition: Taxonomy describes an OTU
Enumerated_Domain_Value_Definition_Source: Author defined

Metabarcoding of Feces of Pacific Walruses and Autosomal DNA Sequence Data of Marine Invertebrates, 2012-2015, Alaska.

Metadata: